EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS


Biresh Kumar Sarkar, Ravi Kumar, Reeta ., S. C. Verma, Shashi Pal, Ramaiah Maddi, C. Murali Krishna, Ravindra Singh

Abstract


Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.

Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.

Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.

Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.

 


Keywords


Anti-inflammatory, Phyllanthus Fraternus, HPTLC, Cyclooxygenase, Lipoxygenase

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About this article

Title

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

Keywords

Anti-inflammatory, Phyllanthus Fraternus, HPTLC, Cyclooxygenase, Lipoxygenase

DOI

10.22159/ijcpr.2017v9i5.22328

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 198-200

Online ISSN

0975-7066

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Authors & Affiliations

Biresh Kumar Sarkar
Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
India

Ravi Kumar
Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
India

Reeta .
Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
India

S. C. Verma
Principal Scientific Officer (Phyto/Org. Chemistry), PLIM Campus, Ghaziabad, U. P., India
India

Shashi Pal
Manav Bharati University, Solan, HP, India
India

Ramaiah Maddi
Department of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A. P., India
India

C. Murali Krishna
Regional Ayurveda Research Institute for Skin disorders, (CCRAS, Ministry of AYUSH) Vijayawada, India
India

Ravindra Singh
Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India, New Delhi 110058


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