EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

  • Biresh Kumar Sarkar Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • Ravi Kumar Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • Reeta . Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • S. C. Verma Principal Scientific Officer (Phyto/Org. Chemistry), PLIM Campus, Ghaziabad, U. P., India
  • Shashi Pal Manav Bharati University, Solan, HP, India
  • Ramaiah Maddi Department of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A. P., India
  • C. Murali Krishna Regional Ayurveda Research Institute for Skin disorders, (CCRAS, Ministry of AYUSH) Vijayawada, India
  • Ravindra Singh Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India, New Delhi 110058

Abstract

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.

Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.

Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.

Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.

 

Keywords: Anti-inflammatory, Phyllanthus Fraternus, HPTLC, Cyclooxygenase, Lipoxygenase

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How to Cite
Sarkar, B., R. Kumar, R. ., S. Verma, S. Pal, R. Maddi, C. Krishna, and R. Singh. “EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 5, Sept. 2017, pp. 198-00, doi:10.22159/ijcpr.2017v9i5.22328.
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