MICROSCOPIC, PHYSICOCHEMICAL AND PHYTOCHEMICAL SCREENING OF CRATEVA MAGNA (LOUR) DC. (LEAF)

  • Abirami J. Department of Biochemistry, Srimad Andavan Arts and Science College, (Autonomous), Trichy
  • Jothi G. Department of Biochemistry, Srimad Andavan Arts and Science College, (Autonomous), Trichy
  • Brindha P. Associate Dean and Co ordinator, CARISM, SASTRA University, Thanjavur

Abstract

Objective: Preliminary screening of phytochemicals is a valuable step, in the detection of the bioactive principles present in medicinal plants and subsequently may lead to drug discovery and development. In the present study, the identity, purity and strength of Crateva magna (Lour) DC. (Leaf), HPTLC, LC MS and phytochemical screening were analysed.

Methods: Tests for Identity, Purity, and Strength of Crateva magna (Lour) DC. (Leaf) Botanical standards, Physicochemical constants such as Foreign matter, Total ash, moisture content, Acid insoluble ash, and water soluble ash and Extractive value such as Hexane, Chloroform, Ethyl acetate, Alcohol were determined. The Preliminary Phytochemical Analysis of various extracts was also determined. HPTLC, LCMS ANALYSIS was analysed.

Results: Microscopic studies T S of Crateva magna leaf shows the presence of prominent midrib, upper and lower epidermis and lamina. Preliminary phytochemical analysis of dry powder and aqueous extracts of Crateva magna (Lour) DC. (Leaf) showed the presence of flavones, steroids, glycosides, sugar, alkaloids, quinones, phenols, coumarin and lignin. HPTLC of an Aqueous extract of the Crateva magna (Lour) DC. (Leaf). LC MS spectrum indicates may be the presence of bioactive compounds.

Conclusion: The results revealed that aqueous extract of Crateva magna (Lour) DC. (Leaf) has suitable for anti ulcer study.

Keywords: Alkaloids, Flavonoids, Crateva magna (Lour) DC

References

1. Ngari EW, Chiuri LW, Kariuki ST, Huckett S. Ethnomedicine of ogiek of river Njoro Watershed. Ethnobot Res Aplications 2010;8:135-52.
2. Njoroge GN, Bussmann RW. Ethnotherapeutic management of skin diseases 80 ISSN: 2229-3469 (Print) AR Florence. Among the Kikuyus of Central Kenys. J Ethnopharmacol 2007;111:303–7.
3. Farnsworth NR. Preliminary phytochemical screening and HPLC analysis of flavonoid from the methanolic extract of leaves of Annona squamosa. Screening plants for new National Academy Press: Washington, DC; 1988. p. 83-97.
4. Himesh S, Sarvesh S, Sharan PS, Mishra K. Preliminary phytochemical screening and HPLC analysis of flavonoid from the methanolic extract of leaves of Annona squamosa. Int Res J Pharm 2011;5:242-6.
5. Shirwaikar A, Setty M, Bommu P. Effect of lupeol isolated from Crataeva nurvala Buch Ham stem bark extract against free radical induced nephrotoxicity in rats. Indian J Exp Biol 2004;42:686-90.
6. A book of Medicinal plants by S. N Yoganarasimhan, Tamilnadu; 2000. p. 159.
7. Govindarajan, Vijayakumar M, Pushpangadan P. “Antioxidant approach to disease management and the role of ‘Rasayana herbs of Ayurveda”. J Ethnopharmacol 2005;99:165-78.
8. Wahida B, Abderrahman B, Nabil C. Antiulcerogenic activity of Zizyphus lotus (L) extracts. J Ethnopharmacol 2007;112:228-31.
9. Sass JE. Elements of botanical microtechnique. MC Graw Hill Book Co. Newyork; 1940. p. 222.
10. Anonymous. The Ayurvedic pharmacopoeia of India, Government of India, Ministry of Health and Family welfare, Department of Indian system of medicine and Homeopathy, New Delhi; 2001;1:142-3.
11. Harborne JB. Phytochemical methods, a guide to modern technique of plant analysis. 2nd edition. Chapman and Hall UK; 1991. p. 72.
12. Harborne JB. Phytochemical methods, a guide to modern technique of plant Analysis. New york: Chapman and Hall; 1984. p. 1-89.
13. Figueirinha A, Paranhos A, Perez-Alonso JJ. Cymbopogon citratus leaves: characterization of flavonoids by HPLC-PDA-ESI/MS/MS and an approach to their potential as a source of bioactive polyphenols. Food Chem 2008;110:718-28.
14. Rosenbloom RA, Chaudhary J, Castro-Eschenbach D. Traditional botanical medicine: an introduction. Am J Ther 2011;18:158–61.
Statistics
183 Views | 666 Downloads
How to Cite
J., A., J. G., and B. P. “MICROSCOPIC, PHYSICOCHEMICAL AND PHYTOCHEMICAL SCREENING OF CRATEVA MAGNA (LOUR) DC. (LEAF)”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 5, Sept. 2017, pp. 201-4, doi:10.22159/ijcpr.2017v9i5.22564.
Section
Original Article(s)