• G. Srividya Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science Tirupati, Andhrapradesh
  • K. Adilaxmamma Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science Tirupati, Andhrapradesh
  • C. H. Srilatha Department of Veterinary Pathology, College of Veterinary Science Tirupati, Andhrapradesh


Objective: To study the hepatoprotective activity of an aqueous and alcoholic extract of Acorus calamus rhizomes against the paracetamol induced hepatotoxicity in rats.

Methods: Hepatotoxicity was induced by oral administration of paracetamol and chemical parameters such as Glutathione peroxidase, Glutathione reductase, Glutathione, Catalase, lipid peroxidation and histopathological changes in liver was studied by comparing with Silymarin, a standard hepatoprotective drug.

Results: Treatment of rats with aqueous and alcoholic extract of Acoruscalamus rhizome after paracetamol administration normalized the altered levels of above parameters which may comparable with Silymarin and Vit-E. The hepatoprotective activity was confirmed by histopathological examination of the liver tissue of control and treated animals.

Conclusion: Based on the result it can be concluded that Acorus calamus rhizome possesses hepatoprotective effect against paracetamol-induced hepatotoxicity in rats.

Keywords: Acorus calamus rhizome, Paracetamol, Silymarin, Vit-E, Hepatoprotection


1. [Last accessed on 20 Dec 2017]
2. Kulkarni PH. Ayurveda Herbs: Indian Medical Science Series No.106; 1998. p. 73.
3. Gilani AU, Shah AJ, Ahmad M, Shaheen F. Antispasmodic effect of Acoruscalamus Linn is mediated through calcium channel blockade. Phytother Res 2006;20:1080-4.
4. Ghosh S, Sharma AK, Kumar S, Tiwari SS, Rastogi S, Srivastava S. In vitro and in vivo efficacy of acoruscalamus extract against rhipicephalus (Boophilus) microplus. Parasitol Res 2011;108:361–70.
5. Rafatullah S, Tariq M, Mossa JS, Al-yahya MA, Al Said MS, Ageel AM. Antisecretagogue, antiulcer and cytoprotective properties of Acoruscalamus in rats. Fitoterapia 1994;65:1, 19-23.
6. Parab RS, Mengi SA. Hypolipidemic activity of Acoruscalamus L. in rats. Fitoterapia 2002;73:451-5.
7. Gaw MC, Jager AK, Van Staden J. Isolation of β-asarone an antibacterial and anthelmintic compound from Acoruscalamus in South Africa. South Afr J Bot 2002;68:31-5.
8. Pradeep K, Vinay K, Ali MM, Maurya RR, Handa SS, Srimal RC. Protective effect of Acoruscalamus against Acrylamide Induced Neurotoxicity. Phytother Res 2002;16:256-60.
9. Suryadevara P, Khanan P. Screening of plant extracts for larvicidal activity against Culexquinquefasciatus. J Nat Remedies 2002;2:186-8.
10. Ulyana MA, Daniel EA, Jun M, Michael HN, Edward J. Antioxidant capacities of ten edible North American plants. Phytother Res 2002;16:63-5.
11. Rani AS, Satyakala M, Murty USN. Acoruscalamus linn: an important medicinal plant and source of potent biopesticide. Proceedings of the National Academy of Science India Section B, Biological Sciences 2003;73:1-15.
12. Mehrotra S, Mishra KP, Maurya R, Srimal RC, Yadav VS, Pandey R, et al. Anticellular and immunosuppressive properties of ethanolic extract of Acoruscalamus rhizome. Int Immunopharmacol 2003;3:53-61.
13. Yende SR, Harle UN, Bore VV, Bajaj AO, Shroff KK, Vedal YD. Reversal of neurotoxicity induced cognitive impairment associated with Phenytoin and Phenobarbital by Acoruscalamus in mice. J Herbal Med Toxicol 01/2009;3:111–5.
14. Jaripa-Begum, Sohrab H, Yusuf M, Chowdary JU, Husaain MM, Begum HA, et al. In vitro antifungal activity of azaron isolated from the rhizome extract of Acoruscalamus L. Pakistan J Biol Sci 2004;7:1376-9.
15. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Laboratory Clin Med 1967;77:158-9.
16. Raghuramulu. Manual of laboratory techniques NIN ICMR Jamia Osmania, Hyderabad; 1983. p. 204-6.
17. Britton C, Machlly AC. Assay of catalase and peroxidises. Methods Enzymol 1956;136:765-8.
18. Yagi R. Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem 1984;95:351-8.
19. Snedecor WG, Cochran GW. Statistical methods. 8th ed. Iowa State University Press, Ames, USA; 1994.
20. Kumar G, Banu GS, Pandian. Evaluation of antioxidant activity of Trianthemaportulcastrum. Indian J Pharmacol 2005;37:331-3.
21. Ahmed MB, Khater MR. Evaluation of the protective potential of Ambrosia martima extract on acetaminophen-induced liver damage. J Ethnopharmacol 2001;75:169-74.
22. Manikandan S, Sheeladevi R. Antioxidant poperty of ά-asarone against noise stress induced changes in different regions of rat brain. Pharmacol Res 2005;52:467-74.
23. Kumar G, Sharmila Banu G, Vakannan V, Rajasekhara Pandian M. Antihepatotoxic effect of β-carotene on paracetamol induced hepatic damage in rats. Indian J Exp Biol 2004;43:351-5.
24. Suhail M, Ahmad I. In vivo effects of actaminophen on rat RBC and role of vitamin E. Indian J Exp Biol 1995;33:269-71.
25. Acuna UM, Atha DE, Ma J, Nee MH, Kennelly EJ. Antioxidant capacities of ten edible North American plants. Phytother Res 2002;161:63-5.
26. Yu BP. Cellular defences against damage from reactive oxygen species. Physiology reviews 1994;74:279-318.
27. Rajesh B, Parames CS. The protein fraction of Phyllanthusniruri plays a protective role against acetaminophen induced hepatic disorders via its antioxidant properties. Phytother Res 2006;20:595-601.
28. Kim ST, Kim JD, Ahn SH, Ahn GS, Lee YIK, Jeong YS. Hepatoprotective and antioxidant effects of Alnusjaponica extracts on acetaminophen induced hepatotoxicity in rats. Phytother Res 2004;18:971-5.
29. Linn, Hsu YF, Lin TC, Hsu My. Antioxidant and hepatoprotective effects of punicalagin and punicalin on acetaminophen induced liver damage in rats. Phytother Res 2001;15:206-21.
30. Shaiakhmetova HM, Kovalenko VM, Bondarenko LB, Kuzemenko IV. Antioxidant effectiveness of short-chain alpha tocopherol acetate in acute paracetamol poisoning in rats. Ukr Biokhinm Zh 2000;73:61-7.
31. Suresh Kumar SV, Sujatha C, Syamala J, Nagasudha B, Mishra SH. Protective effect of root extract of Operculinaturpethum Linn against paracetamol induced hepatotoxicity in rats. Indian J Pharm Sci 2006;68:32-5.
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How to Cite
Srividya, G., K. Adilaxmamma, and C. H. Srilatha. “PROTECTIVE EFFECT OF ACORUS CALAMUS RHIZOME IN PARACETAMOL EXPOSURE INDUCED HEPATOTOXICITY IN RATS: BIOCHEMICAL AND HISTOPATHOLOGICAL STUDY”. International Journal of Current Pharmaceutical Research, Vol. 10, no. 3, May 2018, pp. 7-10, doi:10.22159/ijcpr.2018v10i3.27218.
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