• Khetbadei Lysinia Hynniewta Hadem Division of Animal Health, Indian Council of Agricultural Research, North-East Headquarter Region Umiam 793103, Meghalaya, India
  • Arnab Sen Division of Animal Health, Indian Council of Agricultural Research, North-East Headquarter Region Umiam 793103, Meghalaya, India



Aristolochia tagala, Oxidative stress, Antioxidant activity


Objective: Study of antioxidant activity of a crude aqueous-methanol extract of Aristolochia tagala and its fractions and identification of the compounds with antioxidant activity.

Methods: The antioxidant activity was assayed by the ability to scavenge free radicals such as superoxide, nitric oxide and ABTS radical cation and the identification of compounds was carried out by LC/MS analysis.

Results: Fraction I of Aristolochia tagala showed the highest free radical scavenging activity and compounds responsible for its activity were identified as magnoflorine, apigenin dimethyl ether, aristolone, and N-trans-feruloyldopamine.

Conclusion: The free radical scavenging property of the compounds present in Aristolochia tagala may be one mechanism that contributed to medicinal property exhibited by this plant.


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Valko M, Leibfritz D, M Jan, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.

Szumiel I. Ionizing radiation-induced oxidative stress, epigenetic changes and genomic instability: the pivotal role of mitochondria. Int J Radiat Biol 2014;91 Suppl 1:1-12.

Kakehashi A, Wei M, Fukushima S, Wanibuchi H. Oxidative stress in the carcinogenicity of chemical carcinogens. Cancers 2013;5:1332-54.

Ercal N, Gurer-Orhan H, Aykin-Burns N. Toxic metals and oxidative stress part 1:mechanism involved in metal-induced oxidative damage. Curr Top Med Chem 2001;1 Suppl 6:529-39.

Omar HEM. Mycotoxins-induced oxidative stress and disease. Available from: [Last accessed on 10 Mar 2018]

Noda N, Wakasugi H. Cancer and oxidative stress. Asian Med J 2001;44 Suppl 12:1571-4.

Fuchs Tarlovsky V. Role of antioxidants in cancer therapy. Nutrition 2013;29:15-21.

Ullah A, Khan A, Khan I. Diabetes mellitus and oxidative stress-a concise review. Saudi Pharm J 2016;24:547-53.

Gracia KC, Llanas-Cornejo D, Husi H. CVD and oxidative stress. J Clin Med 2017;6 Suppl 2:22.

Kim GH, Kim JE, Rhie SJ, Yoon S. The role of oxidative stress in neurodegenerative diseases. Exp Neurobiol 2015;24 Suppl 4:325-40.

Li S, Chen G, Zhang C, Wu M, Wu S, Liu Q. Research progress of natural antioxidants in foods for the treatment of diseases. Food Sci Human Wellness 2014;3:110-6.

Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4 Suppl 2:89-96.

Brewer MS. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf 2011;10:221-7.

Sati SC, Sati N, Rawat U, Sati OP. Medicinal plants as a source of antioxidants. Res J Phytochem 2010;4 Suppl 4:213-34.

Hadem KLH, Sharan RN, Kma L. Phytochemicals of Aristolochia tagala and Curcuma caesia exert an anticancer effect by tumor necrosis factor-α-mediated decrease in nuclear factor kappa B binding activity. J Basic Clin Pharma 2016;7:1-11.

Sreejayan S, Rao MNA. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49:105–7.

Syiem D, Sharma R, Saio V. In vitro antioxidant potential of some traditionally used medicinal plants of North-east India and assessment of their total phenolic content. Pharmacology-online 2009;3:952-65.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radical Biol Med 1999;26:1231-7.

Basaiyye SS, Naoghare PK, Kanojiya S, Bafana A, Arrigo P, Krishnamurthi K, et al. Molecular mechanism of apoptosis induction in Jurkat E6-1 cells by Tribulus terrestris alkaloids extract. J Tradit Complement Med 2017;1-10.

Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 2015;11 Suppl 8:982-91.

Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176-86.

Li C, Wang MH. Potential biological activities of Magnoflorine: a compound from Aristolochia debilis Sieb. et Zucc. Korean J Plant Res 2014;27 Suppl 3:223-8.

Zulkefli HN, Mohamad J, Abidin NZ. Antioxidant activity of methanol extract of Tinospora crispa and Tabernaemontana corymbosa. Sains Malays 2013;42 Suppl 6:697-706.

Hung TM, Lee JP, Min BS, Choi JS, Min KNA, Zhang XF, et al. Magnoflorine from Coptidis rhizoma protects high-density lipoprotein during oxidant stress. Biol Pharm Bull 2007;30 Suppl 6:1157-60.

Krishna MS, Joy B, Sundaresan A. Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L. J Food Sci Technol 2015;52 Suppl 6:3561-70.

Wang J, Zhao J, Liu H, Zhou L, Liu Z, Wang J, et al. Chemical analysis and biological activity of the essential oils of two valerianaceous species from China: Nardostachys chinensis and Valeriana officinalis. Molecules 2010;15:6411-22.

Dizdar M, Vidic D, Pozgan F, Stefane B, Maksimovi´c M. Acetylcholinesterase inhibition and antioxidant activity of N-trans-Caffeoyldopamine and N-trans-Feruloyldopamine. Sci Pharm 2018;86:11.

Hadem KLH, Sharan RN, Kma L. Inhibitory potential of methanolic extracts of Aristolochia tagala and Curcuma caesia on hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice. J Carcinog 2014;13:7.

Anilkumar ES, Mathew D, Nishanth Kumar S, Dileep Kumar BS, Latha PG. A comparative study on in vitro antioxidant, anticancer and antimicrobial activity of the methanol extracts of the roots of four species of Aristolochia L. from southern Western Ghats of India. Int J Adv Res 2014;2 Suppl 12:153-64.

Battu GR, Parimi R, Shekar KBC. In vivo and in vitro pharmacological activity of Aristolochia tagala (syn: Aristolochia acuminata) root extracts. Pharm Biol 2011;49 Suppl 11:1210-4.



How to Cite

Hadem, K. L. H., and A. Sen. “IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF ARISTOLOCHIA TAGALA”. International Journal of Current Pharmaceutical Research, vol. 10, no. 4, July 2018, pp. 40-42, doi:10.22159/ijcpr.2018v10i4.28459.



Original Article(s)