PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF FRUITS OF ANNONA RETICULATA AGAINST STANDARD PATHOGENIC STRAINS


Sujata Paul, Biswajit Dash, Aditya Jyoti Bora, Bikash Gupta

Abstract


Objective: To evaluate the anti-microbial activity of ethanolic extract of fruits of Annona reticulata (family-Annonaceae) in conjugation with phytochemical analysis.

Methods: The ethanolic extract of fruits of Annona reticulata (family-Annonaceae) was prepared by Soxhlet extraction and analysed for phytochemical constituents using standard methods. The anti-microbial activity of the plant extract was examined against bacterial strains and fungal strains using disc diffusion method.

Results: The present investigation shows the phytochemical analysis, anti-microbial activity of the ethanolic extract of the plant Annona reticulata. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, steroids, proteins and amino acids and tannins. The anti-microbial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.

Conclusion: The present study concluded that ethanolic extract of fruits of the Annona reticulata contain high presence of phytochemicals. The ethanolic extract of the plant was found to possess promising anti-microbial activity when compared with the standards.

Keywords


Annona reticulata, Anti-microbial activity, Disc diffusion method, Staphylococcus aureus, Escherichia coli and Candida albicans

| PDF | HTML |

References


Francesco VD, Giorgio F, Hassan C, Manes G, Vannella L, Panella C, et al. Pylori antibiotic resistance: a systematic review. J Gastrointestin Liver Dis 2010;19:409-14.

Woodford N, Livermore DM. Infections caused by gram-positive bacteria: a review of the global challenge. J Infect 2006;59:1-16.

Tripathi KD. Antimicrobial drugs. Essentials of medicinal pharmacology. Jayplee Brothers Medical Publishers; 2008. p. 668-9.

Dubovskiy IM, Martemyanov VV, Vorontsova YL, Rantala MJ, Gryzanova EV, Glupov VV. Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut o galleria melloneolla L. Larvae (Lepidoptera, Pyralidae). Comp Biochem Physiol Part C: Toxicol Pharmacol 2008;148:1-5.

Willey JM, Sherwood LM, Woolverton CM. Microbiology (7th ed.) McGraw-Hill, New York; 2008.

Hong LS, Ibrahim D, Kassim J, Sulaiman S. Gallic acid: an anticandidal compound in hydrolysable tannin extracted from the barks of Rhizophora apiculata Blume. J Appl Pharm Sci 2011;1:75-9.

Abirami S, Kannan N, Poonkothai M. Antimicrobial activity and phytochemical screening of the leaf extracts of Eucalyptus globules. Int J Curr Pharm Res 2017;9:85-9.

Farnsworth NR. Ethno pharmacology and future drug development: The North American experience. J Ethno-pharmacol 1993;38:145–52.

Houghton PJ. The role of plants in traditional medicine and current therapy. J Alter Complement Med 1995;1:131–43.

Mahesh B, Satish S. Antimicrobial activity of some important medicinal plants against plant and human pathogens. World J Agric Sci 2008;4(S):839-43.

Janakia A, Kaleena K, Elumalai D, Hemalatha P, Velu K, Sudha R. Phytochemical screening, antioxidant and antibacterial activities of Millingtonia hortensis. Int J Curr Pharm Res 2017;9:162-7.

Rahman SM, Rashedul MI, Rahman S, Mosaiab T, Ahmed R, Khatun F, et al. Antihyperglycemic studies with methanol extract of Annona reticulata L. (Annonaceae) and Carissa carandas L. (Apocynaceae) leaves in Swiss Albino mice. Adv Nat Appl Sci 2011;5:218–22.

Mondal SK, Mondal NB, Mazumder UK. In vitro cytotoxic and human recombinant caspase inhibitory effect of Annona reticulata leaves. Indian J Pharmacol 2007;39:253–4.

Islam MR, Rahman SM, Ahmed M, Das PR, Islam MT, Kabir MH, et al. Antinociceptive activity studies with methanol extract of Annona reticulata L. (Annonaceae) and Carissa carandas L. (Apocynaceae) leaves in swiss albino mice. Adv Nat Appl Sci 2012;6:1313–8.

Bhalke RD, Chavan MJ. Analgesic and CNS depressant activities of extracts of Annona reticulata Linn. bark. Phytopharmacology 2011;1:160–5.

Chavan MJ, Kolhe DR, Wakte PS, Shinde DB. Analgesic and anti-inflammatory activities of kaur-16-en-19-olic acid from Annona reticulata L. bark. Phytother Res 2011;26:273–6.

Suresh HM, Shivakumar B, Shivakumar SI. Inhibitory potential of the ethanol extract of Annona reticulata Linn. against melanoma tumor. J Nat Pharm 2011;2:168–72.

Suresh HM, Shivakumar B, Shivakumar SI. Phytochemical potential of Annona reticulata roots for antiproliferative activity on human cancer cell lines. Adv Life Sci 2012;2:1–4.

Morton J. Sugar apple. Fruits Warm Climate; 1987. p. 69–72.

Ogunwande IA, Ekundayo E, Olawore NO, Kasali AA. Essential oil of Annona reticulate L. leaves from Nigeria. J Essent Oil Res 2006;18:374–6.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci 2011;1;98-106.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016;6:71-9.

Ushimaru PI, Da Silva MTN, Stasi LCD, Barbosa L, Fernandes A JR. Antibacterial activity of medicinal plant extracts. Braz J Microbiol 2007;38:717-9.

Bansal S, Choudhary S, Sharma M, Kumar SS, Lohan S, Bhardwaj V, et al. Tea: a native source of antimicrobial agents. Food Res Int 2013;53:568–84.

Cowan MM. Plant products as antimicrobial agents. Clinical Microbiol Rev 1999;12:564–82.




About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF FRUITS OF ANNONA RETICULATA AGAINST STANDARD PATHOGENIC STRAINS

Keywords

Annona reticulata, Anti-microbial activity, Disc diffusion method, Staphylococcus aureus, Escherichia coli and Candida albicans

DOI

10.22159/ijcpr.2018v10i4.28466

Date

16-07-2018

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 10, Issue 4 (July-Aug), 2018 Page: 59-63

Online ISSN

0975-7066

Authors & Affiliations

Sujata Paul
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017, India

Biswajit Dash
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017, India

Aditya Jyoti Bora
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017, India

Bikash Gupta
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, Assam 781017, India


Refbacks

  • There are currently no refbacks.