PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)


Jafar Ali, Dibyendu Shil

Abstract


Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.

Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).

Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.

Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.

Keywords


Tagetes erecta, Anti-microbial, Agar disc diffusion method, Zone of Inhibition, Minimum Inhibitory Concentration, Phytochemical screening

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About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)

Keywords

Tagetes erecta, Anti-microbial, Agar disc diffusion method, Zone of Inhibition, Minimum Inhibitory Concentration, Phytochemical screening

DOI

10.22159/ijcpr.2018v10i4.28468

Date

16-07-2018

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 10, Issue 4 (July-Aug), 2018 Page: 64-67

Online ISSN

0975-7066

Authors & Affiliations

Jafar Ali
Girijananda Chowdhury Institute of Pharmaceutical Science Azara, Guwahati, India
India

Dibyendu Shil
Girijananda Chowdhury Institute of Pharmaceutical Science Azara, Guwahati, India


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