CYTOTOXICITY OF AROMATIC COMPOUND FROM AN ENDOPHYTIC FUNGUS, CLADOSPORIUM SP. EN-S01

  • R. R. Indry Noviarin Examinati Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia
  • Asri Peni Wulandari Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia
  • Desi Harneti Putri Huspa Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia
  • Poniah Andayaningsih Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia

Abstract

Objective: Endophytes have the potential to synthesize various bioactive secondary metabolites. The aims of the study were to isolate the major compound from endophytic Cladosporium sp. EN-S01., identify its function group, then evaluate its cytotoxicity.

Methods: The endophytic fungus was grown in potato dextrose broth and extracted using ethyl acetate. Secondary metabolites were isolated by chromatographic separation and re-crystallization, and function group was determined by infrared spectroscopy. In vitro cytotoxicity was evaluated by the prestoblue assay.

Result: The isolated aromatic compound evaluated by prestoblue assay against breast cancer cell line MCF-7 showed cytotoxicity with IC50 value of 746,03 ppm.

Conclusion: Our findings indicate this aromatic compound might be useful lead compounds to develop cytotoxic drugs.

Keywords: Cytoxicity, Cladosporium, Endophytic, Fungus, Prestoblue, Aromatic

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Examinati, R. R. I. N., A. P. Wulandari, D. H. Putri Huspa, and P. Andayaningsih. “CYTOTOXICITY OF AROMATIC COMPOUND FROM AN ENDOPHYTIC FUNGUS, CLADOSPORIUM SP. EN-S01”. International Journal of Current Pharmaceutical Research, Vol. 10, no. 6, Nov. 2018, pp. 10-12, doi:10.22159/ijcpr.2018v10i6.30964.
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