EVALUATION OF ANTI-ASTHMATIC ACTIVITY OF METHANOLIC EXTRACT OF BERLERIA PRIONITIS LINN. AERIAL PARTS
Keywords:Anti-asthmatic, Antihistaminic, Anti-inflammatory, Berleria prionitis
Objective: The aim of the present study is to evaluate the aerial parts of a plant of Barleria prionitis Linn., for its antiasthmatic activity with the separation of active moieties.
Methods: Adult wistar albino rats were used for the anti-inflammatory study. Histamine-induced bronchospasm was conducted on isolated goat trachea.
Results: The dried and powdered aerial parts of Berleria prionitis was extracted with continuous soxhlet extraction with Petroleum ether (40-60 Â° C), Chloroform, Ethyl acetate, Acetone, Methanol, and Hydroalcoholic solvents. Preliminary phytochemical screening of all extracts was done. Preliminary animal studies by In vitro isolated goat trachea chain preparation of all extracts were done to find out the potent extract. In this study, the methanolic extract of aerial parts of Berleria prionitis was found to be potent comparative to another extract. The results of carrageenan induced rat paw oedema model indicated the dose-dependant anti-inflammatory activity. As compared to standard drug (Indomethacin), methanolic extract showed similar activity which was found to be statistically significant (P<0.0001). The extent of DPPH radical scavenging was determined by calculated the IC50 value of methanolic extract Berleria prionitis (133.5) compared with the Ascorbic acid (114.7) taken as standard. In the present study, the histamine-induced dose-dependent contraction of goat tracheal chain was significantly inhibited (p<0.001) by methanolic extract of aerial parts of Berleria prionitis (200 Î¼g/ml). Thus the present study revealed that the methanolic extract of Berleria prionitis (MEBP) has significant antihistaminic (H1 receptor antagonist) activity.
Conclusion: In view the fact that tribal have well experienced the antiasthmatic effects of the roots of Barleria prionitis Linn. The results of our study, for the first time, show that the methanolic extract of aerial parts of Berleria prionitis Linn. possesses antioxidant, anti-inflammatory, Bronchodilator properties and therefore can be used for the antiasthmatic treatment.
Aloke Gopal Ghoshal, GD Ravindran, Paras Gangwal, Girish Rajadhyaksha, Sang-Heon Cho. The burden of segregated respiratory disease in India and the quality of care in these patients: results from the Asia-Pacific burden of respiratory diseases study. Lung India 2016;33:611-9.
Ajeet Singh, Navneet. A review: traditional, ethnomedicinal utilization, pharmacological properties, and phytochemistry of Barleria prionitis Linn. Int J Pharm Sci Rev Res 2017;44:19-26.
Kamini Singh, Deepika Sharma, Gupta Rs. A comprehensive review on Barleria Prionitis (L.). Asian J Pharm Clin Res 2017;10:22-9.
Sachin Parmar, Amit Gangwal, Navin Sheth. Evaluation of the antiasthmatic activity of a polyherbal formulation containing four plant extracts. J Curr Pharma Res 2010;2:40-4.
J Shetty Akhila, Shyamjith, Deepa, MC Alwar. Acute toxicity studies and determination of median lethal dose. Curr Sci 2007;93:7.
Alam MM, Anis M. The medicinal plants of gunung leuser national park, Indonesia. J Ethnopharmacol 1987;19:85-8.
Alzweiri M, Sarhan AA, Mansi K, Hudaib M, Aburjai T. Ethnopharmacological survey of medicinal herbs in Jordan, the Northern Badia region. J Ethnopharmacol 2011;137:27â€“35.
Aneja KR, R Joshi, C Sharma. The potency of berleria prionitis L. bark extracts against oral diseases causing strains of bacteria and fungi of clinical origin. New York Sci J 2010;3:5-12.
Aqil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish J Biol 2006;30:177â€“83.
Banerjee D, Maji A, Banerji P. Barleria prionitis linn: a review of its traditional uses, phytochemistry, pharmacology and toxicity. Res J Phytochem 2012;6:31-41.
Chen JL, Blanc P, Stoddart CA, Bogan M, Rozhon EJ, Parkinson N, et al. New iridoids from the medicinal plant Barleria prionitis with potent activity against the respiratory syncytial virus. J Nat Prod 1998;61:1295-7.
Kapoor A, Shukla S, Kaur R, Kumar R, Lehra KS, Kapoor S. Preliminary phytochemical screening and antioxidant activity of whole plant of Barleria prionitis Linn. Int J Adv Pharm Biol Chem 2014;3:410-9.
Khadse CD, Kakde RB. Anti-inflammatory activity of aqueous extract fractions of Barleria prionitis L, roots. Asian J Plant Sci Res 2011;1:63-8.
Maji AK, Bhadra S, Mahapatra S, Banerji P, Banerjee D. Mast cell stabilization and membrane protection activity of Barleria prionitis L. Pharmacogn J 2011;3 Suppl 24:67-71.
Sharma P, Sharma GN, Shrivastava B, Jadhav HR. Evaluation of antioxidant potential of Barleria prionitis Leaf and stem. Am J Phytomed Clin Ther 2014;2:1177-86.
Singh B, Bani S, Gupta DK, Chandan BK, Kaul A. Anti-inflammatory activity of TAF an active fraction from the plant Barleria prionitis Linn. J Ethnopharmacol 2003;85:187-93.
Trease, William C Evans. Textbook of Pharmacognosy. 16 edition. Elesvier publication; 2009.