EVALUATION OF IN VITRO ANTI-UROLITHIATIC ACTIVITY OF METHANOLIC EXTRACT OF CUCUMIS MELO SEEDS ON CALCIUM OXALATE CRYSTALS
Objective: This in vitro study was carried out to evaluate the anti-urolithiatic activity of the methanolic extract of the Cucumismelo seeds on experimentally prepared calcium oxalate crystals which was prepared by the homogeneous precipitation method in the laboratory.
Methods: The crude extract was prepared by the soxhlet extraction method and the extraction was done until all the compounds get extracted into the solution and solvent was evaporated by rotary evaporator. Extracts were stored in an airtight light-resistant container at 4 Â°C in a refrigerator for further analysis.
Results: Seed extract of Cucumismelo showed maximum efficiencies in the dissolution of the calcium oxalate crystals. Cystone drug was used as the standard. This in vitro study has shown that the methanolic extract of the seeds of Cucumismelo has the potential anti-urolithiatic activity when compared with the standard.
Conclusion: This in vitro study has given the primary evidence that the extract of seeds of Cucumismelo has the anti-urolithiatic activity. In vivo studies can be carried out on the seed extract of Cucumismelo for further investigations.
2. Vijaya T, Sathish Kumar M, Ramarao NV, Narendra Babu A, Ramarao N. Urolithiasis and its causesâ€“short review. J Phytopharmacol 2013;2:1-6.
3. Pethiyagoda A, Pethiyagoda K. Descriptive evaluation of ureteric urolithiasis between genders. Int J Sci Res Publications 2016;6:47-50.
4. Atodariya U, Barad R, Upadhyay S, Upadhyay U. Anti-urolithiatic activity of Dolichosbiflorus seeds. J Pharmacogn Phytochem 2013;2:209-13.
5. Niharika M, Himabindu J, Ramanjaneyalu K. Evaluation of in vitro antiurolithiatic activity of Tridaxprocumbens. Int J Sci Res 2018;7:93-8.
6. Jha R, Ramani P, Patel D, Desai S, Meshram D. Phytochemical analysis and in vitro urolithiatic activity of leaves (DC) Baker. J Med Plants Studies 2016;4:18-22.
7. WWW.urologyspecialistsatlanta.com. [Last accessed on 10 Aug 2018].
8. Abeygunasekara AM. Urinary stone disease in Sri Lanka. Ceylon Medical Journal 2011;29:41-3.
9. Perera ND, Perera JS. The role of extracorporeal shock wave lithotripsy in renal calculi. Sri Lanka J Surgery 2014;31:6-12.
10. Sharma D, Dey YN, Sikarwar I, Sijoria R, Wanjari MM, Jadhav AD. In vitro study of aqueous leaf extract of Chenopodium album for inhibition of calcium oxalate and brushite crystallization. Egyptian J Basic Appl Sci 2016;3:164-71.
11. Akinyeye AJ, Solanke EO, Adebiyi IO. Phytochemical and antimicrobial evaluation of leaf and seed of Moringaoleifera extracts. Int J Res Med Health Sci 2014;4:2307-83.
12. Bijauliya RK, Alok S, Jain, S. K., Singh, V. K. and Singh, D., 2017. Herbal and allopathic medicine for kidney, gallbladder, and urinary stones: a review. Int J Pharm Sci Res 1935. p. 8.
13. Johri N, Cooper B, Robertson W, Choong S, Rickards D, Unwin R. An update and practical guide to renal stone management. Nephron Clin Practice 2010;116:c159â€“71.
14. Kumar BN, Kumar GK, Srinivasa V, Syed Bilal. A review on urolithiasis: Int J Universal Pharm Life Sci 2012;2:269-80.
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