ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS
Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity.
Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin.
Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules.
Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity.
2. Khan R, Islam B, Akram M, Shakil S, Ahmad A, Ali SM, et al. Anti-microbial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 2009;14:586-97.
3. Santos PRV, Oliveira ACX, Tomassini TCB. Contole microbiogico de products fitoterapicos. Rev Farm Bioquim 1995;31:35-8.
4. Mahesh B, Satish S. Anti-microbial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci 2008;4:839-43.
5. Renu G, Bandana T, Pushpendra S, Singh HB, Sharma VD, Katoch VM, et al. Anti-tuberculosis activity of selected medicinal plants against multi-drug resistant Mycobacterium tuberculosis isolates. Indian J Med Res 2010;131:809-13.
6. Rosangela DP, Vincenzo DF, Francesco V. In vitro anti-microbial activity of essential oil from Mediterranean Apiaceae, Verbenaceae and Lamiaceae against food borne pathogens and spoilage bacteria. Ann Microbiol 2005;55:139-43.
7. Burt S. Essential oils their anti-microbial properties and potential application in foods-a review. Int J Food Microbiol 2004;94:223-53.
8. Faleiro ML, Miguel MG, Laderio F. Anti-microbial activity of essential oils isolated from portuguese endemic species of thymus. Lett Appl Microbiol 2003;36:35-40.
9. Kaveri S, Vandana T, Rajneesh P. Study of anti-microbial activity of medicinal plants against various multiple drug resistance pathogens and their molecular characterization and its bioinformatics analysis of antibiotic gene from genomic database with degenerate primer prediction. Int J Biol Technol 2010;1:15-9.
10. Flora O, Folasade D. Anti-microbial effect of phyllanthus amarus and parquetina nigrescens on salmonella typhi. Afr J Biomed Res 2008;11:215-9.
11. Mallappa KS, Mohd SA, Uma Rani S. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. J Evidence Based Complementary Altern Med 2016;1-21. http://dx.doi.org/10.1155/2016/3012462
12. Esmail Abdollahzadeh, Masoud Rezaei, Hedayat Hosseini. Antimicrobial activity of plant essential oils of thyme essential oil, nisin, and their combination to control Listeria monocytogenes inoculated in minced fish meat. Food Control 2014;35:177-83.
13. Hugo WB, Russell AD. Pharmaceutical microbiology. 5th ed Blackwell Scientific Publications, Oxford, London; 1992. p. 258-97.
14. Flora Oluwafemi, Folasade Debiri. Antimicrobial effect of phyllanthus amarus and parquetina nigrescens on salmonella typhi. Afr J Biomed Res 2008;11:215-9.
15. Aristides ML, Edeltrudes OL, Evandro LS. Inhibitory effect of ?-pinene, ?-pinene and eugenol on the growth of growth of potential infectious endocarditis causing gram-positive bacteria. Braz J Pharm Sci 2007;43:121-6.
16. Amber Adams, Satyanshu Kumar, Marck Clauson, Shivendra Sahi. Anti-yeast activities of origanum oil against human pathogenic yeasts. Adv Biosci Biotechnol 2011;2:103-7.
17. Deriu A, Zanetti S, Sechi LA, Marongiu B, Piras A, Porcedda S, et al. Antimicrobial activity of Inula helenium L. essential oil against Gram-positive and gram-negative bacteria and candida spp. Int J Antimicrobial Agents 2008;31:588-90.
18. Lahlou M. Methods to study the phytochemistry and bioactivity of oils. Phytother Res 2004;18:435-88.
19. Eugenia P, Maria JG, Carlos C, Ligia S. Antifungal activity of Thapsia villosa essential oil against candida, cryptococcus, malassezia, aspergillus and dermatophyte species. Molecules 2017;22:1-11.
20. Bachir RG, Benali M. Antibacterial activity of these essential oils from the leaves of Eucalyptus globulus against Escherichia coli and staphylococcus aureus. Asian Pac J Trop Biomed 2012;2:739-42.
21. Meena TI, Jolly CI, Sheela D. A study of the phytochemical composition and antibacterial activity of Holestemma adakodien schultes. Int J PharmTech Res 2011;3:1208-10.
22. Changhong Liu, Mishra AK, Bing He, Renxiang Tan. Antimicrobial activities of isoalantolactone, a major sesquiterpene lactone of Inula racemose. Chinese Sci Bull 2001;46:498-501.
23. Lokhande PD, Gawai KR, Kodam KM, Kuchekar BS. Anti-bacterial activity of isolated constituents and extract of roots of Inula racemosa. Rea J Med Plant 2007;1:7-12.
24. Sini Sadasivan, Latha PG, Sasikumar JM, Rajashekaran S, Shyamal S, Shine VJ. Hepatoprotective studies on hedyotis corymbosa (L) lam. J Ethanopharmacol 2006;106:245-9.
25. Warrier PK, Nambiar VPK, Ramankutty C. Indian medicinal plants-a compendium of 500 species. Vol. 3. Orient Longman Ltd. Chennai; 1995. p. 120-3.
26. Tamana S, Abdur RM, Ahad AM, Samsuddin FM. Hepatoprotective and antibacterial activity of ursolic acid extracted from hedyotis corymbosa L. Bangladesh J Sci Ind Res 2010;45:27-34.
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