PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM
Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis.
Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method).
Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs.
Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics.
2. Maruti J, Dhanavade CB, Jalkute JS, Ghosh Kailash DS. Study antimicrobial activity of lemon. Br J Pharmacol Toxicol 2011;2:119-22.
3. Behboud J, Amirreza K. Antibacterial activities methanol of extract and lemongrass essence on pathogenic bacteria. World Appl Sci J 2013;28:1796-801.
4. Brahma Chari UN. Herbal remedy. Curr Sci 2001;81:15-6.
5. Zitterl Eglseer K, Sosa S, Jurenitsch J, Tubaro A, Franz C. Anti-edematous activities of the main triterpendiol esters of marigold. J Ethnopharmacol 1997;57:139-44.
6. Arbonnier M. Trees, shrubs and lianas of West African dry zones. Editions Quae; 2004.
7. Oyen LP. Adenium obesum (Forssk.) Roem and schult. Plant Resources Trop Africa 2008;11:46-9.
8. Win NK, Back CG, Kim YH, Jung HY. Desert rose witches’ broom disease associated with ‘Candidatus Phytoplasmaaurantifolia’. J Gen Plant Pathol 2012;78:73-6.
9. Rowley GD. Caudiciform and pachycaul succulents: pachycauls, bottle-, barrel-and elephant-trees and their kin, a collector's miscellany. Mill Valley: Calif., Strawberry Press; 1987. p. xiii, 282p.-illus.
10. Mclaughlin J, Garofalo J. The desert rose, Adenium obesum: nursery production. Fact sheet; 2002.
11. Mclaughlin J, Garofalo J. The desert rose, Adenium obesum: nursery production. Fact sheet; 2002.
12. Paul D, Biswas K, Sinha SN. Biological activities of adenium obesum (Forssk.) roem and schult: a concise review. Malaya J Biosci 2015;2:214-21.
13. Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nat Rev Drug Discovery 2014;2:379-86.
14. http://www.businessdictionary.com/definition/pathogens.html [Last accessed on 05 Oct 2018]
15. Parta AK. Dietary phytochemicals. Publisher spirzyeer; 2008. p. 301-6.
16. http://www.businessdictionary.com/definition/pathogens.html [Last accessed on 05 Oct 2018]
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