EXPLORATION OF IN VIVO ANTIOXIDANT ACTIVITY OF 50% ETHANOLIC EXTRACT OF SESAMUM INDICUM L. SEED AGAINST HIGH FAT DIET INDUCED RATS
Objective: The aim of the present study was to investigate the in vivo antioxidant potential of 50% ethanolic extract of Sesamum indicum against high-fat diet-induced rats.
Methods: Animals were treated with plant extract for 30 d, and a high-fat diet was given to all groups except plain control, throughout, out the study. And alpha-tocopherol acetate (Vit, E) was used as standard. Pre-treatment with 16 mg/100 gm of body weight of 50% ethanolic extract of Sesamum indicum improved the Superoxide dismutase, catalase, glutathione, and lipid peroxidation levels significantly as compared to control group.
Results: The present studies revealed that Sesamum indicum has significant in vivo antioxidant activity and can be used to protect tissue from oxidative stress. The result showed that the activities of SOD, catalase, lipid peroxidase, and glutathione, in the group treated with high-fat diet declined significantly than that of normal group.
Conclusion: 50% ethanolic extract of in the dose of Sesamum indicum 16 mg/100 gm of body weight, has improved the SOD, catalase, glutathione, and lipid peroxidase levels significantly, which were comparable with high-fat-diet-induced rats. Based on this study we conclude that the 50% ethanolic extract of Sesamum indicum possesses in vivo antioxidant activity and can be employed in protecting tissue from oxidative stress.
2. Chopra IC, Handa KL, Kapur LD. Indigenous drugs of India. 2nd ed. U. N. Dhur and Sons Private Limited, 15, Bankim Chatterjee Street Calcutta-12; 1958. p. 569.
3. Bhattacharjee SK. Hand book of medicinal plants. 4th edn. Pointer Publishers, Jaipur; 2004. p. 320.
4. Nadkarni KM. Indian materia medica. 3rd ed. Popular Book Depot Bombay 7, Dhootapapeshwar Prkaashan Ltd; 1982. p. 1126-9.
5. Evans WC. Trease and evans pharmacognosy 16th ed. Saunders Elsevier Edinburgh London New York Philadelphia St Louis Sydney Toronto; 2009. p. 190.
6. Ghani MN. Khazayinul Advia. 1st ed. Central council for Research In Unani Medicine, Ministry of Health and Family Welfare, Govt. of India, New Delhi; 2010. p. 180-5.
7. Khory RN, Katarak NN. Materia medica of India and therapeutics. 3rd Reprint ed. Pub. Neeraj Publishing House Delhi-110052; 1993. p. 462.
8. Namiki M. Nutraceutical functions of sesame: a review. Crit Rev Food Sci Nutr 2007;47:651–73.
9. Hirose N, Inoue T, Nishihara K, Sugano M, Akimoto K, Shimizu S, et al. Inhibition of cholesterol absorption and synthesis in rats by sesamin. J Lipid Res 1991;32:629-38.
10. Bedigian D, Harlan JR. Evidence for cultivation of sesame in the ancient world. Econ Bot 1986;40:137-54.
11. Shahidi F, Liyana Pathirana CM, Wall D. Antioxidant activity of white and black sesame seeds and their hull fractions. Food Chem 2006;99:478-83.
12. Liu Z, Saarinen NM, Thompson LU. Sesamin is one of the major precursors of mammalian lignans in sesame seed (Sesamum indicum) as observed in vitro and in rats. J Nutr 2006;136:906-12.
13. Ghafoorunissa, Hemalatha S, Rao MV. Sesame lignans enhance antioxidant activity of vitamin E in lipid peroxidation systems. Mol Cell Biochem 2004;262:195-202.
14. Costa FT, Neto SM, Bloch C, Franco OL. Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels. Curr Microbiol 2007;55:162-6.
15. Yokota T, Matsuzaki Y, Koyama M, Hitomi T, Kawanaka M, Enoki Konishi M. Sesamin, a lignan of sesame, downregulates cyclin D1 protein expression in human tumor cells. Cancer Sci 2007;98:1447-53.
16. Visavadiya NP, Narasimhacharya AV. Sesame as a hypocholesteraemic and antioxidant dietary component. Food Chem Toxicol 2008;46:1889-95.
17. Ashakumary L, Rouyer I, Takahashi Y, Ide T, Fukuda N, Aoyama T. Sesamin, a sesame lignan, is a potent inducer of hepatic fatty acid oxidation in the rat. Metabolism 1999;48:1303-13.
18. Nakano D, Kurumazuka D, Nagai Y, Nishiyama A, Kiso Y, Matsumura Y. Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats. Clin Exp Pharmacol Physiol 2008;35:324-26.
19. Lee CC, Chen PR, Lin S, Tsai SC, Wang BW, Chen WW. Sesamin induces nitric oxide and decreases endothelin-1 production in HUVECs: possible implications for its antihypertensive effect. J Hypertens 2004;22:2329-38.
20. Kirtikar KR, Basu BD. Indian medicinal plants. Pub. International Book Distributers, Dehradun; 1995. p. 1858-61.
21. Dymock W, Warden CJH, Hooper D, Pharmacographia Indica. A history of the principal drugs. The institute of health and tibbi research, hamdard national foundation, Pakistan; 1972. p. 337-8.
22. Freirich EJ, Gehan EA, Rall DP, Schmidt LH, Skipper HE. Quantitative comparison of toxicity of anti ulceragents in mouse, rat, hamster, dog, monkey and man. Cancer Chemother Rep 1966;50:219-44.
23. Kimuru Y, Rhminani H, Okuola H. Effect of extract of oyster on lipid metabolism in rats. J Ethnopharmacol 1998;59:117-23.
24. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499-502.
25. Malstrom B, Andreasson L, Reinhammer B. In: The enzymes. Boyer P. editor XIIB. Academic Press: New York; 1975. p. 533.
26. Fridovich I. Biological aspects of Superoxide radical and superoxide dismutase. Biochemical and Medical Aspects of Active Oxygen. Asada K, Hayaish O. Editor. Baltimore: University Park Press; 1977. p. 171-6.
27. Aebi HE. Catalase in vivo. Methods in enzymology. L Packer. editor. N. York: Academic Press; 1884. p. 121.
28. Yagi K. Simple assay for the level of total lipid peroxidation in serum or plasma metods in molecular. Biology 1998;108:101-6.
29. Armstrong D, Browne R. A systemic approach to laboratory technology, clinical correlation and antioxidant therapy. Free Radicals Diagnostic Med 1994;366:43-58.
30. Spiteller G. Lipid peroxidation in aging and age-dependent diseases. Exp Geront 2001;36:1425-57.
31. Evans P, Halliwell B. Free radicals and hearing, causes, consequence and criteria. Ann N Y Acad Sci 1999;884:1940.
32. Noeman SA, Hamooda HE. Biomedical study of oxidative stress markers in liver, kidney and heart of high fat diet induced obesity in rats. Diabetol Metab Syndrome 2011;3:1-17.
33. Patel C, Ghanim H, Ravishankar S, Sia CL, Viswanathan P, Mohanty P, et al. Prolonged reactive oxygen species generation and nuclear factor-kappaB activation after a high-fat high-carbohydrates meal in the obese. J Clin Endocrinol Metab 2007;92:4476-9.
This work is licensed under a Creative Commons Attribution 4.0 International License.