ANALYSIS OF BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF PRUNUS CERASUS (SOUR CHERRY)
Keywords:Antioxidant, DPPH, FRAP, Prunus cerasus, Flavonoids
Objective: Prunus cerasus L. is consumed as a dietary supplement to promoting health benefits. The purpose of this study was to investigate the phenol and flavonoids content of sour cherry methanol extract in terms of antioxidant activity.
Methods: The content of polyphenols and flavonoids was quantified. DPPH and FRAP assay were used to evaluate the radical scavenging properties and reducing the antioxidant power of sour cherry fruit.
Results: DPPH scavenging activity was evaluated IC50 10.32±2.23 mg/ml and FRAP was estimated at 205±30 µmol/g.
Conclusion: Sour cherry methanol extract includes various bioactive compounds like polyphenols, flavonoids and anthocyanidins, which can be valuable beneficial effects to the prevention of various vascular diseases.
2. Mulabagal V, Lang GA, DeWitt DL, Dalavoy SS, Nair MG. Anthocyanin content, lipid peroxidation and cyclooxygenase enzyme inhibitory activities of sweet and sour cherries. J Agric Food Chem 2009;57:1239-46.
3. Wang H, NairMG, Strasburg GM. Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries. J Nat Prod 1999;62:294–6.
4. Wojdy?o A, Figiel A, Lech K, Nowicka P, Oszmian?ski J. Effect of convective and vacuum-microwave drying on the bioactive compounds, colour and antioxidant activity of sour cherries. Food Bioprocess Technol 2014;7:829?41.
5. Khoo GM, Clausen MR, Pedersen H, Larsen E. Bioactivity and total phenolic content of 34 sour cherry cultivars. J Food Comp Anal 2001;24:772?6.
6. Rice Evans C. Screening of phenolics and flavonoids for antioxidant activity. In: Packer L, Hiramatsu M, Yoshikawa T. eds. Antioxidant Food Supplements in Human Health. SanDiego, Calif: Academic Press; 1999. p. 239–53.
7. Kamei H, Kojima S, Hasegawa M, Umeda T, Terabe K, Yukawa T. Suppressive effect of flavonoid extracts from flower petals on cultured human malignant cells. J Clin Exp Med 1993;164:829–30.
8. Bomser J, Madhavi DL, Singletary K, Smith MA. In vitro anticancer activity of fruit extracts from Vaccinium species. Planta Med 1996;62:212–6.
9. Wang H, Nair MG, Iezzoni A, Strasburg GM, Booren AM, Gray JI. Quantification and characterization of anthocyanins in balaton tart cherries. J Agric Food Chem 1997;45:2556–60.
10. Wang H, Nair MG, Strasburg GM. Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries. J Nat Prod 1999;62:294–6.
11. Kang SY, Seeram NP, Nair MG, Bourquin LD. Tart cherry anthocyanins inhibit tumor development in ApcMin mice and reduce proliferation of human colon cancer cells. Cancer Lett 2003;194:13–9.
12. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555–9.
13. V Lopez, S Akerreta, E Casanova, JM Garcia Mina, RY Cavero, MI Calvo. In vitro antioxidant and antirhizopus activities of Lamiaceae herbal extracts. Plant Foods Hum Nutr 2007;62:151–5.
14. Oyaizu M. Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Japan J Nutr 1986;44:307–14.
15. Bomser J, Madhavi DL, Singletary K, Smith MA. In vitro anticancer activity of fruit extracts from Vaccinium species. Planta Med 1996;62:212–6.