IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS
Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli.
Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC).
Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria.
Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.
2. Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol 2003;80:223-30.
3. Aono R, Ito M, Horikoshi K. Measurement of cytoplasmic pH of the alkaliphile Bacillus lentus C-125 with a fluorescent pH probe. Microbiology 1997;143:2531-6.
4. Azziz Baumgartner E, Lindblade K, Gieseker K, Rogers HS, Kieszak S, Njapau H, et al. Case-control study of an acute aflatoxicosis outbreak, Kenya, Environ Health Perspect 2005;113:1779–83.
5. Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: an ethnomedicinal plant. J Adv Pharm Technol Res 2011;2:104–9.
6. Caleja C, Barros L, Antonio AL, Carocho M, Oliveira MB, Ferreira IC. Fortification of yogurts with different antioxidant preservatives: a comparative study between natural and synthetic additives. Food Chem 2016;210:262–8.
7. Clarke D, Tyuftin AA, Cruz Romero MC, Bolton D, Fanning S, Pankaj SK. Surface attachment of active antimicrobial coatings onto conventional plastic-based laminates and performance assessment of these materials on the storage life of vacuum-packaged beef sub-primals. Food Microbiol 2017;62:196-201.
8. Clementi EA, Marks LR, Roche Hakansson H, Hakansson AP. Monitoring changes in membrane polarity, membrane integrity, and intracellular ion concentrations in Streptococcus pneumoniae using fluorescent dyes. J Visualized Exp 2014;84:1-8.
9. Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen. Arabian J Chem 2019;12:3075-86.
10. Dhanani T, Shah S, Gajbhiye NA, Kumar S. Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arabian J Chem 2017;10:S1193-S1199.
11. Fernandez Lopez J, Zhi N, Aleson Carbonell L, Perez Alvarez JA, Kuri V. Antioxidant and antibacterial activities of natural extracts: application in beef meatballs. Arabian J Chem 2005;59:3431-55.
12. Mau JL, Chen CP, Hsieh PC. Antimicrobial effect of extracts from Chinese chive, cinnamon, and corni fructus. J Agric Food Chem 2001;49:183–8.
13. Molina Gutierrez A, Stippl V, Delgado A, Ganzle MG, Vogel RF. In situ determination of the intracellular pH of Lactococcus lactis and Lactobacillus plantarum during pressure treatment. Appl Environ Microbiol 2002;68:4399-406.
14. Oonmetta Aree J, Suzuki T, Gasaluck P, Eumkeb G. Antimicrobial properties and action of galangal (Alpinia galanga linn.) on Staphylococcus aureus. LWT Food Sci Technol 2006;39:1214-20.
15. Sanchez E, Garcia S, Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholera. Appl Environ Microbiol 2010;76:6888-94.
16. Gurnani N, Gupta M, Shrivastava R, Mehta D, Mehta B. Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of Capsicum frutescens L. Indian J Nat Prod Resour 2016;7:32-9.
17. Mostafa AA, Al-Askar AA, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci 2018;25:361-6.
This work is licensed under a Creative Commons Attribution 4.0 International License.