PHYTOCHEMICAL EVALUATION OF ARTOCARPUS HIRSUTUS LAM. FRUIT EXTRACT: A POTENTIAL WILD ENDEMIC PLANT
Keywords:Artocarpus hirsutus, Bioactive compounds, Ethnomedicine, Wild, Mineral, HPTLC, Endemic, Moraceae, Phenolic acid, Phytochemicals
Objective: The present study includes the qualitative and quantitative estimation of phytochemical constituents and HPTLC (High-performance thin layer chromatography) analysis of phenolic acids in the fruit extract of Artocarpus hirsutus.
Methods: Qualitative and quantitative analysis of Artocarpus hirsutus methanol fruit extract using standard methods. HPTLC analysis was performed by the CAMAG HPTLC system (Switzerland) for gallic acid, caffeic acid and ferulic acid determination.
Results: The Preliminary phytochemical analysis revealed the presence of phytoconstituents such as flavonoids, phenols, tannins, steroids, glycosides, alkaloids, carbohydrate and protein. The methanol fruit extract has high carbohydrate content (267±0.02 mg/g FW). And good amount of protein, phenol, flavanoid were also found. The results of mineral studies shows that elements such as potassium (1.601%) and nitrogen (1.4%) were present in higher quantity. The result showed the presence of caffeic acid within peak 7 with an Rf (Retension factor) value 0.49, ferulic acid within peak 8 with an Rf value 0.60, and gallic acid within peak 5 with an Rf value 0.25.
Conclusion: The results indicated that the Artocarpus hirsutus fruit contains an appreciable amount of bioactive compounds.
2. Shrestha PM, Dhillion SS. Diversity and traditional knowledge concerning wild food species in a locally managed forest in Nepal. Agroforestry Syst Kluwer Academic Publishers; 2006.
3. Ajesh TP, Naseef SAA, Kumuthakalavalli R. Ethnobotanical documentation of wild edible fruits used by muthuvan tribes of idukki, Kerala-India. Int J Pharm Bio Sci 2012;3:479–87.
4. Dibinlal D, Sheethadevi B. Anti-ulcer activity of the bark of Artocarpus hirsutus. J Pharm Res 2013;8:3-5.
5. Beecher. Phytonutrients' role in metabolism: effects on resistance to degenerative processes. Nutr Rev 1999;57:S3-S6.
6. Ananda S, Tragoolpua K, Chantawannakul P, Tragoolpua Y. Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods. Asian Pac J Cancer Prev 2013;14:6991-5.
7. Kokate CK. Practical pharmacognosy. Vallabh Prakashan, Delhi; 2000.
8. Sadasivam S, Manickam A. Biochemical methods. 3rd edn. New Age International Publishers, New Delhi, India; 2008.
9. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin’s phenol reagent. J Biol Chem 1951;193:265-75.
10. Makkar HPS. Quantification of tannins in tree and shrub foliage: a laboratory Mannual. Dondrecht. The Netherlands: Kluwer Academic; 2003.
11. Toth SJ, Prince AL, Wallace A, Mikkelsen DS. Rapid quantitative determination of eight mineral elements in plant tissue systematic procedure involving use of a flame photometer. Soil Sci 1948;66:459-66.
12. Johnson JT, Iwang EU, Hemen JT, Odey MO, Efiong EE, Eteng OE. Evaluation of anti-nutrient contents of watermelon Citrullus lanatus. Ann Biol Res 2012;3:5145-50.
13. Paulsamy S, Jeeshna MV. Preliminary phytochemistry and antimicrobial studies of an endangered medicinal herb Exacum bicolour Roxb. Res J Pharm Biol Chem Sci 2011;2:447-57.
14. Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008;8:634-46.
15. Hendra R, Ahmad S, Sukari A, Shukor MY. Flavanoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci 2011;12:3422-31.
16. Nayak BS, Pereira LM. Catharanthus roseus flower extract has wound-healing activity in s prague dawley rats. BMC Complement Altern Med 2006;6:1-6.
17. Rathee P, Chaudhary H, Rathee S, Rathee D. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets 2009;8:229-35.
18. Onibon VO, Abulude FO, Lawal LO. Nutritional and antinutritional composition of some Nigerian fruits. J Food Tech 2007;5:120-2.
19. Sarma BK, Singh UP. Ferulic acid may prevent infection by Sclerotium rolfsii in Cicer arietinum. World J Micro Biotech 2003;19:123-7.
20. Maurya S, Rashmi S, Singh DP, Srivastava J, Singh SP. Phenolic compounds of sorghum vulgare in response to sclerotium rolfsii infection. J Pl Inter 2007;2:25-29.
21. Soong YY, Barlow P. Antioxidant activity and phenolic content of selected fruit seeds. Food Chem 2004;88:411-7.