ANALYSING THE BIOCATALYTIC ASPECT OF AMYLASE FROM PLANT AND MICROBIAL SOURCES FOR INDUSTRIAL APPLICATION
Objective: Biocatalysts have a wide role in industries and amylases are one of the best enzymes used in various industries as they stay stable physically and chemically even under environmental or physical stress. Extraction of amylase from plant sources like chickpea and green pea and also the microbial source of Bacillus subtilis.
Methods: Enzymatic activity was determined by enzyme assay and purified through the following steps such as salt precipitation, dialysis, Ion-exchange chromatography and characterized using SDS PAGE.
Results: Enzymatic activity of amylase produced by bacillus subtilis was determined the highest (4600 U/ml) activity and molecular weight 48.9kDa was recorded.
Conclusion: Amylase is a highly important enzyme that breaks down the starch molecules into simple sugars. It has potential in a wide range of industrial processes such as food, fermentation and pharmaceutical industries. All the sources used in this study proves that the bacteria have the highest concentration of enzyme in the form of protein.
2. Hill R, Needham J. the chemistry of life: eight lectures on the history of biochemistry. London: Cambridge University Press; 1970. p. 17.
3. Abhishek Walia, Ayesh Gaur, Neeraj Rana. ?-amylases from microbial sources and its potential applications in various industries. Natl Acad Sci Lett India 2013;36:9–17.
4. TV Jaya, LV Venkataraman. Changes in the carbohydrate constituents of chickpea and green gram during germination. Food Chem 1981;7:95-104.
5. John Robyt, Dexter French. Action pattern and specificity of amylase from Bacillus subtilis. Arch Biochem Biophys 1963;100:451-67.
6. S Sudharhsan, S Senthilkumar, K Ranjith. Physical and nutritional factors affecting the production of amylase from species of Bacillus isolated from spoiled food waste. Afr J Biotechnol 2007;6:430–5.
7. OH Lowry, NJ Rosebrough, AL Farr, RJ Randall. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75.
8. UK Laemmli. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–5.
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