COMBINED EFFECT OF SELECTIVE BIOACTIVE COMPOUNDS FROM PLANT ORIGIN IN AN ANIMAL MODEL OF ANXIETY
Objective: Evidence is emerging that specific combinations of bioactive compounds may be far more effective in protecting against several diseases as compared to the effect of a single compound. The present study was aimed to investigate the interactive effect of Diosgenin and Silymarin, the bioactive compounds from plant origin in an animal model of anxiety.
Methods: Albino wistar rats of either sex were divided into five groups and treated for 5 d. Group I and II served as control and standard and test groups were treated with Diosgenin (100 mg/kg, p. o.), Silymarin (100 mg/kg, p. o.) and combination of Diosgenin (50 mg/kg, p. o.)+Silymarin (50 mg/kg, p. o.), respectively. Diazepam (1 mg/kg, i. p.) was used as a standard for the study. Anxiolytic effects were studied in the Elevated plus-maze, Hole-board test and Light/Dark model.
Results: The results suggested that Diosgenin when given alone at a dose of 100 mg/kg, does not shown significant anxiolytic effect when as compared with control. Whereas, the compound Silymarin (100 mg/kg) shown significant anti-anxiety effect (P<0.01), independently. The same two bioactive compounds, given in combination at a dose of 50 mg/kg, (each), exhibited significant anxiolytic-like effect, potentially.
Conclusion: It can be concluded that Diosgenin has got a synergistic effect on anti-anxiety action when given in combination with Silymarin.
2. Tanwani H, Nyati P, Atal S, Churihar R. Evaluation of antianxiety, antidepressant and sedative effects of nimodipine in swiss albino mice. Int J Pharm Pharm Sci 2016;8:260-3.
3. Sarwar R, Farooq U, Naz S, Khan A, Bukhari SM, Khan H, et al. Isolation and characterization of two new secondary metabolites from Quercus incana and their antidepressant and anxiolytic-like potential. Front Pharmacol 2018;9:298.
4. Breda van SGJ, Briede JJ, Kok de TMCM. Improved preventive effects of combined bioactive compounds present in different blueberry varieties as compared to single phytochemical. Nutrients 2019;11:61.
5. Manivannan J, Arunagiri P. Sivasubramanian J, Balamurugan E. Diosgenin prevents hepatic oxidative stress, lipid peroxidation and molecular alterations in chronic renal failure rats. Int J Nutr Pharmacol Neutol Dis 2013;3:3.
6. Burczynski FJ, Yan J, Gong Y, Nuguyen D, Wang G, Burczynski SD, et al. The hepatoprotective effect of diltiazem and silymarin. Nat Prod Chem Res 2013;1:111.
7. Hellerbrand C, Schattenberg JH, Peterburs P, Lechner A, Brignoli R. The potential of silymarin for the treatment of hepatic disorders. Clin Phytosciences 2016;2:7.
8. El-Marasy SA, Abd-Elsalam RM, Ahmad-Fariq OA. Ameliorative effects of silymarin on scopolamine-induced dementia in rats. Maced J Med Sci 2018;6:1215-24.
9. Qin Y, Wu X, Huang W, Gong G, Zhao Y, Li D, et al. Acute toxicity and sub-chronic toxicity of steroidal saponins from Dioscorea zingiberensis C. H. wright in rodents. J Ethnopharmacol 2009;126:543-50.
10. Rana P, Agarwal S, Agarwal R. Prostate cancer chemoprevention by silymarin: bench to bedside. Mol Carcinog 2006;45:436-42.
11. Dashputre NL, Naikwade NS. Immunomodulatory activity of Abutilon indicum Linn on albino mice. Int J Pharm Sci Res 2010;1:178-84.
12. Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in rat. J Neurosci methods 1985;14:149-67.
13. Boissier SR, Simon P. Dissociation de deux composantes dans le comportement d investigation de la souris. Arch Int Pharmacodyn Ther 1964;147:372-88.
14. Costall B, Domeney AM, Gerrard PA, Kelley ME, Naylor RJ. ‘Zacopride’ anxiolytic profile in rodent and primate models of anxiety. J Pharm Pharmcol 1988;40:302-5.
15. Mody I, Pearce RA. Diversity of inhibitory neurotransmission through GABA (A) receptors. Trends Neurosci 2004;27:569-75.
16. Dawson GR, Tricklebank MD. Use of the elevated plus-maze in the search for novel anxiolytic agents. Trends Pharmacol Sci 1995;16:33-6.
17. Takeda H, Tsuji M, Matsumiya T. Changes in head?dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol 1998;350:21-9.
This work is licensed under a Creative Commons Attribution 4.0 International License.