ASSESSMENT OF ANTIMICROBIAL EFFICACY OF KOHL/KAJAL PREPARED BY DIFFERENT INDIAN METHODS AGAINST SELECTED MICROBIAL STRAINS

  • DHEERAJ S. RANDIVE Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India 313003
  • SOMNATH D. BHINGE Rajarambapu College of Pharmacy Kasegaon, Walwa, Sangli Maharashtra India 415404
  • NAMDEO R. JADHAV Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India 416013
  • MANGESH A. BHUTKAR Rajarambapu College of Pharmacy Kasegaon, Walwa, Sangli Maharashtra India 415404
  • MRUNAL K. SHIRSAT Dean and Professor Pacific College of Pharmacy, PAHER University Udaipur 313003

Abstract

Objective: To prepare and evaluate different types of Kajal formulations and evaluation of its antimicrobial activity along with preliminary verification of the content responsible for the said effect.


Methods: We have prepared kajal formulations by use of different metal plates, marble tile, ghee and Aloe vera mucilage and tried to verify the antimicrobial effect attributed to the formulation by these substances.


Results: Carbon soot obtained from the use of copper plate showed more antimicrobial potential against Staphylococcus aureus, Pseudomonas aeruginosa and E. coli, with zones of inhibition 18±0.235 mm, 17±0.124 mm and 19±0.528 mm respectively. Also this formulation at different concentrations when compared with Ciprofloxacin exhibited promising results. Moreover, this formulation when used with Ciprofloxacin at a concentration of (50:50) revealed a synergistic effect against the clinically resistant strains of P. aeruginosa, with zone of inhibition 22±0.578 mm and 20±0.987 mm at a concentration of 10 and 5 µg ml-1 respectively, whereas, Ciprofloxacin exhibited zone of inhibition of 26±0.457 mm and 24±0.751 mm at the similar concentrations. To assess the effectiveness of Aloe vera we used marbles tiles for collection of carbon soot. The zones of inhibition observed for Kohl formulations prepared by using carbon soot collected from marble tiles impregnated with Aloe vera mucilage exhibited less antimicrobial activity than that of copper soot against the selected microbial strains.


Conclusion: All the prepared kajal formulations exhibited antimicrobial activity. Aloe vera and copper soot is responsible for significant antimicrobial activity and when combined with Ciprofloxacin it showed synergistic activity against the clinically resistant strains of P. aeruginosa.

Keywords: Kajal, Carbon soot, Aloe vera, Antimicrobial activity, Synergistic activity

References

1. Randive DS, Bhinge SD, Wadkar GH, Bhutkar MA. Comparative standardization of marketed formulations of fermented biomedicine–arjunaristha. Ind J Pharm 2016;27:220–5.
2. Randive DS, Sayyad SF, Bhinge SD, Bhutkar MA. Preparation of Arjun?ri??a using microbes isolated from woodfordia fruticosa flowers (dhayati). Anc Sci Life 2016;36:42-7.
3. Bhinge SD, Bhutkar MA, Randive DS, Wadkar GH, Kamble SY, Kalel PD, et al. Formulation and evaluation of polyherbal gel containing extracts of Azadirachta indica, Adhatoda vasica, Piper betle, Ocimum tenuiflorum and Pongamia pinnata. J Res Pharm 2019;23:44-54.
4. Gandhi BM, Shankar PDS. Current trends and challenges faced in ocular drug delivery systems. Int J Res Phar Chem 2012;2:801-5.
5. Hero F, Salah A. Effect of some plant extracts on isolated bacteria from eyelids of natural eye liner users and eye cosmetics users. J Appl Pharm Sci 2012;2:3-8.
6. Mohta A. Kajal (kohl)-a dangerous cosmetic. Omn J Opht 2010;3:100-1.
7. Ali A, Ali AR, Khalid T, Adnan El-Y. Kohl-the traditional eyeliner: use and analysis. Ann Sau Med 1993;13:26-30.
8. Maher MAE, Mohamed AK, Rabia AGH, Daw AM, Mahmud ASA. Chemical and physical investigations of arabian eye kohl brands as cosmetic in Libya. J Tur Chem Soc 2017;4:463-76.
9. Srikanth T, Hussen SS, Anand A, Vasantharaju SG, Sandeep. Der Pharm Lett 2011;3:334-41.
10. Gupta R, Hafsa A, Sehgal S, Dwivedi H. Formulation, preliminary evaluation and antimicrobial activity of a herb based kohl. Int J Phytol Nat In 2016;3:1-5.
11. Sampath Kumar KP, Bhowmik D, Harish G, Duraivel S, Pragathikuma B. Ocular inserts: a novel controlled drug delivery system. Pharm Inn J 2012;12:1-19.
12. Abeer Ali A, Elbanat HM, Solafa AH. Determination of lead concentration in cosmetic (kohl). Sud Uni Sci Tech; 2016. p. 1-28.
13. https://www.boldsky.com/beauty/make-up-tips/2017/different-ways-to-make-kajal-at-home/articletrending-pf1001-113125.html [Last accessed on 15 Nov 2018]
14. Sherwal BL, Verma AK. Epidemiology of ocular infection due to bacteria and fungus–a prospective study. J Med Ed Res 2008;10:127-31.
15. Mutimer MN, Riffikin C, Hill JA, Marry E, Glickman CNG. Syn-thesis of methylsilyl derivates of procaine and their diffusion. J Am Pharm Asso Sci 1956;45:212.
16. Bhinge SD, Bhutkar MA, Randive DS, Wadkar GH, Todkar SS. Development and evaluation of antimicrobial polyherbal gel. Annl Pharm Fr 2017;75:349-58.
17. Bhinge SD, Todkar SS, Kakade PM, Kadam PM. Formulation development and evaluation of antimicrobial polyherbal gel. Annl Pharm Fran 2017;75:349–58.
18. Efterpi V, Christaki, Panagiota C, Florou P. Aloe vera: a plant for many uses. J Food Agric Environ 2010;8:245-9.
19. Pankaj KS, Giri DD, Singh R, Pandey P, Gupta S, Shrivastava AK, et al. Therapeutic and medicinal uses of aloe vera: a review. Pharma Pharm 2013;4:599-610.
20. Rajeswari R, Umadevi M, Rahale SC, Pushpa R, Selvavenkadesh S, Sampath Kumar KP, et al. Aloe vera: the miracle plants its medicinal and traditional uses in India. J Pharm Phys 2012;1:118.
21. Sanghi SB. Aloe vera: a medicinal herb. Int J Res Granth 2015;3:32-4.
22. Mohammed L, Sserunjogia, Roger K, Abrahamsena JN. A review paper: current knowledge of ghee and related products. Int Dairy J 1998;8:677-88.
23. Mahakalkar A, Kashyap P, Bawankar RH. The versatility of cow ghee-an ayurveda perspective. Am J Drug Delvery Ther 2014;1:28-34.
Statistics
63 Views | 103 Downloads
Citatons
How to Cite
RANDIVE, D. S., S. D. BHINGE, N. R. JADHAV, M. A. BHUTKAR, and M. K. SHIRSAT. “ASSESSMENT OF ANTIMICROBIAL EFFICACY OF KOHL/KAJAL PREPARED BY DIFFERENT INDIAN METHODS AGAINST SELECTED MICROBIAL STRAINS”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 3, May 2020, pp. 37-44, doi:10.22159/ijcpr.2020v12i3.38302.
Section
Original Article(s)