COMPARISON IMMUNOHISTOCHEMISTRY EXPRESSION OF DESIDUAL NATURAL KILLER (dNK) IN SEVERE PREECLAMPSIAAND NORMAL PREGNANCY
Keywords:Severe preeclampsia, Normal pregnancy, Expression of dNK cell
Objective: Preeclampsia characterized systematically by extensive vascular endothelial dysfunction and microangiopathy on mother, dNK is very important for the success of placentation. They are the key mediator of maternal immune system interactions with fetal cells. dNK cells are also involved in the modulation of EVT and the remodeling of spiral arteries.
Methods: Analytic research with cross-sectional study, with samples of pregnant women who suffer from severe PE and aterm pregnancy which came to H. Adam Malik Hospital and Networking Hospital, November 2015-April 2016. The samples are 46 women, who met the inclusion criteria.
Results: Immunohistochemistry examination dNK cell in the severe PE case group and control group, statistically found p<0,05. dNK placenta expression in the severe preeclampsia case group gives an overview of expression with a mean of 2.55±2.31, while the control group of normal pregnancy had higher mean is 8.66±3.16.
Conclusion: The examination of immunohistochemistry of dNK cells showed there is a significant difference in the expression of Immuno-histochemistry dNK cells between severe PE case group and non severe PE.
2. Sibai B, Dekker G, Kupferminc M. Pre-eclampsia. Lancet 2005;365:785–99.
3. Duley L. The global impact of pre-eclampsia and eclampsia. Semin Perinatol 2009;33:130-7.
4. Fisher SJ. The placental problem: linking abnormal cytotrophoblast differentiation to the maternal symptoms of preeklampsia. Reprod Biol Endocrinol 2004;2:53.
5. Kopcow HD, Karumanchi SA. Angiogenic factors and natural killer (NK) cells in the pathogenesis of preeclampsia. J Reprod Immunol 2007;76:23–9.
6. Eastabrook G. The role of decidual natural killer cells in normal placentation and in the pathogenesis of preeklampsia. Obstet Gynaecol Can 2008;30:467–76.
7. Kumar V, Medhi B. Emerging role of uterine natural killer cells in establishing pregnancy. Iran J Immunol 2008;5:71-81.
8. Kopcow HD, Karumanchi SA. Angiogenic and natural killer (NK) cells in the pathogenesis of preeclampsia. J Reprod Immunol 2007;76:23-9.
9. Milne F, Redman C, Walker J. The pre-eclampsia community guideline (PRECOG): how to screen for and detect onset of preeklampsia in the community. Br Med J 2005;330:576–80.
10. Steegers EAP. Pre-eclampsia. Lancet 2010;376:631–44.
11. Uzan Jennifer. Marie Carbonnel. Olivier Piconne. Pre-eclampsia: pathophysiology, diagnosis, and management. Vasc Health Risk Manage 2011;7:467–74.
12. Ng EH, Chan CC, Tang OS, Yeung WS, Ho PC. The role ofendometrial and subendometrial vaskular ity measured bythree-dimensional power doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles. Hum Reprod 2006;21:1612–7.
13. Burton GJ, Jauniaux E. Placental oxidative stress: from miscarriage to preeklampsia. J Soc Gynecol Investig 2004;11:342–52.
14. Jauniaux E, Watson AL, Hempstock J, Bao YP, Skepper JN, Burton GJ. Onset of maternal arterial blood flow and placental oxidative stress. A possible faktor in human early pregnancy failure. Am J Pathol 2000;157:2111–22.
15. Plasencia W, Maiz N, Bonino S, Kaihura C, Nicolaides KH. Uterine artery Doppler at 11+0 to 13+6 w in the prediction of pre-eclampsia. Ultrasound Obstet Gynecol 2007;30:742–9.
16. Burton GJ. Oxygen, the janus gas; its eff ects on human placental development and function. J Anat 2009;215:27–35.
17. Burton GJ, Yung HW, Cindrova Davies T, Charnock Jones DS. Placental endoplasmic reticulum stress and oxidative stress in the pathophysiology of unexplained intrauterine growth restriction and early onset preeklampsia. Placenta 2009;30(Suppl A):43–8.
18. Jauniaux E, Poston L, Burton GJ. Placental-related diseases of pregnancy: Involvement of oxidative stress and implications in human evolution. Hum Reprod Update 2006;12:747–55.
19. Brosens JJ, Parker MG, McIndoe A, Pijnenborg R, Brosens IA. A role for menstruation in preconditioning the uterus for successful pregnancy. Am J Obstet Gynecol 2009;200:615.
20. Huppertz B. Placental origins of preeklampsia: challenging the current hypothesis. Hypertension 2008;51:970–5.
21. Myers J, Mires G, Macleod M, Baker P. In preeklampsia, the circulating faktors capable of altering in vitro endothelial function precede clinical disease. Hypertension 2005;45:258–63.
22. Irani RA, Xia Y. The functional role of the renin-angiotensin system in pregnancy and preeklampsia. Placenta 2008;29:763–71.