EVALUATION OF POMEGRANATE PEEL AS A SUBSTRATE FOR CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER

  • PAYEL SUTRADHAR Department of Microbiology, Institute of Genetic Engineering, Kolkata-700128, West Bengal, India
  • ISHIKA SAHA Department of Microbiology, Institute of Genetic Engineering, Kolkata-700128, West Bengal, India
  • AMIT CHAKRAVARTY Department of Biotechnology, Institute of Genetic Engineering, Kolkata 700128, West Bengal, India

Abstract

Objective: The present study aimed to evaluate dried pomegranate peels as a substrate for citric acid production by Aspergillus niger.


Methods: The morphological study of Aspergillus niger was carried out by wet mount with lactophenol cotton blue and slide culture method. A preliminary qualitative screening of citric acid-producing ability of this fungal strain was also performed by using the Czapek-Dox agar medium containing Bromocresol green. Dried and finely powdered Pomegranate peel was used as a principal substrate for the production of citric acid by submerged fermentation. Classical method of citric acid recovery involved a precipitation technique using calcium hydroxide followed by filtration and subsequent treatment with sulphuric acid. The citric acid produced was also chemically detected and titrimetrically estimated by 0.1 N NaOH.


Results: The present experiment demonstrated that pomegranate peels may serve as an inexpensive medium for the production of citric acid with a yield of 19.39 g/l by using Aspergillus niger.


Conclusion: This study provided an alternative basis to recycle the fruit peel waste of pomegranate in order to achieve industrially feasible and environmentally sustainable bio-production of pharmaceutically significant citric acid.

Keywords: Aspergillus niger, Citric acid, Pomegranate peel, Czapek-Dox agar, Bromocresol green, Fermentation, GRAS

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SUTRADHAR, P., I. SAHA, and A. CHAKRAVARTY. “EVALUATION OF POMEGRANATE PEEL AS A SUBSTRATE FOR CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 3, May 2020, pp. 61-65, doi:10.22159/ijcpr.2020v12i3.38307.
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