DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TADALAFIL IN BULK AND FORMULATION

Authors

  • SHRISHAIL M. GHURGHURE D. S. T. S. Mandal’s College of Pharmacy Solapur 413004. Maharashtra, India
  • MANISHA S. DYAWARKONDA D. S. T. S. Mandal’s College of Pharmacy Solapur 413004. Maharashtra, India
  • SACHIN YANJANE D. S. T. S. Mandal’s College of Pharmacy Solapur 413004. Maharashtra, India

DOI:

https://doi.org/10.22159/ijcpr.2020v12i3.38310

Keywords:

Tadalafil, DMSO, UV-spectroscopic method

Abstract

Objective: A new simple, sensitive, precise and reproducible spectroscopic method was developed for the determination of Tadalafil in Pharmaceutical formulation with Dimethyl Sulfoxide.

Methods: The UV spectrum of Tadalafil in Dimethyl sulfoxide (DMSO) showed λ max at 285.6 nm. Beer’s law is valid in the concentration range of 10-60µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness.

Results: The method was demonstrated excellent linearity over the range of 10-60µg/ml with regression equation y= 0.0337x-0.1343 and regression correlation R2= 0.998. Moreover, the method was found to be highly sensitive with LOD (2.44µg/ml) and LOQ (7.40µg/ml).

Conclusion: Based on results, the proposed method can be successfully applied for assay of Tadalafil in various pharmaceutical dosage forms.

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Published

2020-05-15

How to Cite

GHURGHURE, S. M., M. S. DYAWARKONDA, and S. YANJANE. “DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TADALAFIL IN BULK AND FORMULATION”. International Journal of Current Pharmaceutical Research, vol. 12, no. 3, May 2020, pp. 74-77, doi:10.22159/ijcpr.2020v12i3.38310.

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