HIBISCUS ROSA SINENSIS LOADED SOLID LIPID NANOPARTICLES AND IN VIVO WOUND HEALING ACTIVITY IN WISTAR ALBINO RATS
Keywords:Solid Lipid Nanoparticles, Hibiscus rosa sinensis, Wound healing, Glyceryl monostearate, Beeswax
Objective: The objective of the present research was to investigate the wound-healing potency of solid lipid nano particles of Hibiscus rosa sinensis extract. Crude herbal extracts and rudimentary formulations containing herbal extracts are good for demonstrating the feasibility of the concept; however, such formulations suffer with poor oral bioavailability and variability within groups of subjects. Converting herbal extracts into novel drug delivery systems may prove effective in addressing some of these problems.
Methods: In the present study an attempt was made to develop Hibiscus rosa sinensis extract loaded solid lipid nanoparticles (HSLNs) using lipids glycerol monostearate (GMS) or beeswax. The prepared HSLNs were characterised for their size, surface charge and morphology. The optimized HSLNs were incorporated into Carbopol gel and tested for wound healing activity in male Wistar albino rats using excision wound model.
Results: HSLNs of ~175 nm in size carrying negative charge were obtained with the optimised procedure using beeswax. The shape of the HSLNs was nearly spherical. The HSLNs (10 mg/ml) treated wounds healed much faster compared to raw crude extract and healing was comparable to marketed preparation.
Conclusion: It is concluded that converting crude herbal extracts into SLNs can be an effective way to enhance the effectiveness of herbal extracts and their in vivo activity.
2. X Chen. Astragaloside IV-loaded nanoparticle-enriched hydrogel induces wound healing and anti-scar activity through topical delivery. Int J Pharm 2013;447:171–81.
3. S Bergin, P Wraight. Silver based wound dressings and topical agents for treating diabetic foot ulcers. Cochrane Database Syst Rev 2006; 25:CD005082.
4. BA Lipsky, C Hoey. Topical antimicrobial therapy for treating chronic wounds. Clin Infect Dis 2009;49:1541–9.
5. JM Dasam U, Natarajan J, Karri VVSR, Wadhwani AD, Antony J. Targeting efficacy of simvastatin for hormone-dependent carcinomas through solid lipid nanoparticles. J Nanomed Nanotechnol 2006;7:1–7.
6. X Yang, Y Liu, C Liu, N Zhang. Biodegradable solid lipid nanoparticle flocculates for pulmonary delivery of insulin. J Biomed Nanotechnol 2012;8:834–42.
7. W Mehnert. Solid lipid nanoparticles production, characterization and applications. Adv Drug Delivery Rev 2001;47:165–96.
8. P Ekambaram, AAH Sathali, K Priyanka. Solid lipid nanoparticles: a review. Int J App Pharm 2012;47:80–102.
9. S Küchler. 3D-wound healing model: influence of morphine and solid lipid nanoparticles. J Biotechnol 2010;148:24–30.
10. S Ghaffari. Nanotechnology in wound healing; semisolid dosage forms containing curcumin-ampicillin solid lipid nanoparticles, in vitro, ex-vivo and in vivo characteristics. Adv Pharm Bull 2018;8:395–400.
11. RV Punna Rao Ravi, N Aditya, Himanshu Kathuria, Srinivas Malekar. Lipid nanoparticles for oral delivery of raloxifene: Optimization, stability, in vivo evaluation and uptake mechanism. Eur J Pharm Biopharm 2014;87:114–24.
12. G Sandri. Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing. Eur J Pharm Biopharm 2013;84:84–90.
13. SH Khalid L, Rizwani GH, Sultana V, Zahid H, Khursheed R. Antidepressant activity of ethanolic extract of Hibiscus rosa sinenesis linn. Pak J Pharm Sci 2014;27:1327–31.
14. DX Hou, X Tong, N Terahara, D Luo, M Fujii. Delphinidin 3-sambubioside, a hibiscus anthocyanin, induces apoptosis in human leukemia cells through reactive oxygen species-mediated mitochondrial pathway. Arch Biochem Biophys 2005;440:101–9.
15. A Herrera Arellano, S Flores Romero, MA Chavez Soto, J Tortoriello. Effectiveness and tolerability of a standardized extract from hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial. Phytomedicine 2004;11:375–82.
16. M Ali, A SH. Hair care and herbal drugs. Indian J Nat Prod 1997;13:3-5.
17. K PN, R VN, JP, Handbook of medicinal plants. New Delhi: Oxford and IBH Publishing Co. Pvt. Ltd; 1979.
18. IE Kate, OO Lucky. The effects of aqueous extracts of the leaves of hibiscus rosa-sinensis linn. on renal function in hypertensive rats. Afr J Biochem Res 2010;4:43–6.
19. P Vijayanand, V Jyothi, N Aditya, A Mounika. Development and characterization of solid lipid nanoparticles containing herbal extract: in vivo antidepressant activity. J Drug Delivery 2018 https://doi.org/10.1155/2018/2908626
20. B Shivananda Nayak, S Sivachandra Raju, FA Orette, AV Chalapathi Rao. Effects of Hibiscus rosa sinensis L (Malvaceae) on wound healing activity: a preclinical study in a sprague dawley rat. Int J Low Extrem Wounds 2007;6:76–81.
21. A Bhaskar, V Nithya. Evaluation of the wound-healing activity of hibiscus rosa sinensis L (Malvaceae) in wistar albino rats. Indian J Pharmacol 2012;44:694.
22. PR Ravi, N Aditya, H Kathuria, S Malekar, R Vats. Lipid nanoparticles for oral delivery of raloxifene: optimization, stability, in vivo evaluation and uptake mechanism. Eur J Pharm Biopharm 2014;87:114–24.
23. B Gonul, D Erdogan, C Ozogul, M Koz, A Babul, N Celebi. Effect of EGF dosage forms on alkali burned corneal wound healing of mice. Burns 1995;21:7–10.
24. K Kiran, M Asad. Wound healing activity of sesamum indicum L seed and oil in rats. Indian J Exp Biol 2008;46:777–82.
25. KP Mohammed Haneefa, A Abraham, R Saraswathi, GP Mohanta, C Nayar. Formulation and evaluation of herbal gel of basella alba for wound healing activity. J Pharm Sci Res 2012;4:1642–8.
26. KA Shah, AA Date, MD Joshi, VB Patravale. Solid lipid nanoparticles (SLN) of tretinoin: potential in topical delivery. Int J Pharm 2007;345:163–71.
27. P Vijayanand, J Patil, MV Reddy. Formulation, characterization and in vivo evaluation of novel edible dosage form containing nebivolol HCL. Brazilian J Pharm Sci 2016;52:179–89.
28. MC Gohel, RK Parikh, SA Nagori, SN Shah, MR Dabhi. Preparation and evaluation of soft gellan gum gel containing paracetamol. Indian J Pharm Sci 2009;71:120–4.
29. J Balasubramaniam, S Kant, JK Pandit. In vitro and in vivo evaluation of the gelrite gellan gum-based ocular delivery system for indomethacin. Acta Pharm 2003;53:251–61.
30. R Aiyalu, A Govindarjan, A Ramasamy. Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model. Brazilian J Pharm Sci 2016;52:493–507.
31. L Panigrahi, S Ghosal, S Pattnaik, L Maharana, B Barik. Effect of permeation enhancers on the release and permeation kinetics of Lincomycin hydrochloride gel formulations through mouse skin. Indian J Pharm Sci 2006;68:205–11.
32. MGB Dantas. Development and evaluation of stability of a gel formulation containing the monoterpene borneol. Sci World J 2016. Doi:10.1155/2016/7394685
33. D Vasudevan, R Rajan. Effect of permeation enhancers on the penetration mechanism of transfersomal gel of ketoconazole. J Adv Pharm Technol Res 2012;3:112.
34. PK Mukherjee, R Verpoorte, B Suresh. Evaluation of in vivo wound healing activity of hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 2000;70:315–21.
35. R Pawar, P Chaurasiya, H Rajak, P Singour, F Toppo, A Jain. Wound healing activity of sida cordifolia linn. in rats. Indian J Pharmacol 2013;45:474.
36. D Mukherjee. Development and characterization of chitosan-based hydrogels as wound dressing materials. J Drug Delivery Sci Technol 2018;46:498–510.
37. S Mukherjee, S Ray, RS Thakur. Solid lipid nanoparticles: a modern formulation approach in drug delivery system. Indian J Pharm Sci 2009;71:349–58.
38. V Jenning, A Lippacher, SH Gohla. Medium scale production of solid lipid nanoparticles (SLN) by high pressure homogenization. J Microencapsul 2002;19:1–10.
39. CWWPDHR Hepburn. The chemistry of beeswax. In: Honeybee Nests, Springer, Berlin, Heidelberg; 2014. p. 319–39.
40. Pharmaceutical formulations and the importance of zeta potential to pharmaceutical formulations with supplier data. Malvern Instruments Ltd; 2005. Available from: https://www.azonano.com/article.aspx?ArticleID=1234. [Last accessed on 15 Dec 2019]
41. AF Kheradmandnia S, Vasheghani Farahani E, Nosrati M. Preparation and characterization of ketoprofen-loaded solid lipid nanoparticles made from beeswax and carnauba wax. Nanomedicine 2010;6:753–9.
42. MG Anthony, A Attama, Christel C. Effect of beeswax modification on the lipid matrix and solid lipid nanoparticle crystallinity. Colloids Surfaces A Physicochem Eng Asp 2008;315:189–95.
43. F Hayati, SM Ghamsari, MM Dehghan, A Oryan. Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study. J Dermatolog Treat 2018;29:593–9.
44. BP Kermany. Carbopol hydrogels for topical administration: Treatement of Wounds; 2010.
45. M Abdulrhman, N Samir, El Barbary, D Ahmed Amin, R Saeid Ebrahim. Honey and a mixture of honey, beeswax, and olive oilpropolis extract in treatment of chemotherapy-induced oral mucositis: a randomized controlled pilot study. Pediatr Hematol Oncol 2012;29:285–92.