SCREENING OF MULTI BIOACTIVE METABOLITES PRODUCING MICROORGANISMFROM GARDEN SOIL OF DR. M. G. R EDUCATIONAL AND RESEARCH INSTITUTE, CHENNAI

  • SENTHILRAJR Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77
  • GEJALAKSHMI S. Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77
  • THARUNKUMAR M. Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77
  • SANTHOSH A. Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77
  • LOKESH V. Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77
  • TANISHA B. A. Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Dr. M.G.R. Educational and Research Institute, Chennai-77

Abstract

Objective: Screening of industrial important bioactive metabolites of Antibiotics, hydrolytic enzymes producing microorganism from garden soil of Dr. M. G. R Educational and Research Institute, Chennai.


Methods: Desired ten soil samples taken and were serially diluted. Crowded plate method used for antibiotic-producing microorganism and Starch agar medium and gelatin medium tests were performed for hydrolytic enzymes (Amylase and Gelatinase).


Results: Among the tested soil samples, antibiotic producing microorganisms were not found, but has hydrolytic enzymes amylase and gelatinase. Thus screened soil samples biochemically identified as Bacillus species.


Conclusion: This study concludes that, the collected sample, produced Antibiotic negative result and it’s possess other industrial important hydrolytic enzymes. Thus Screening of more bioactive metabolites producing ability from a single isolate, will be more useful for effective screening.

Keywords: Antibiotic activity, Soil microorganisms, Antimicrobial activity and Hydrolytic enzyme

References

1. David Taylor. The pharmaceutical industry and the future of drug development. In. Pharmaceuticals in the Environment; 2015. p. 1-33.
2. Abbas S, Senthilkumar R, Arjunan S. Isolation and molecular characterization of the microorganism producing novel antibiotics from the soil sample. Eur J Exp Biol 2014;4:149-55.
3. Berdy J. Bioactive microbial metabolites. J Antibiot 2005;58:1–26.
4. JJ Sanglier, H Haag, TA Huck, T Fehr. Review of actinomycetes compounds: 1990–1995. Expert Opin Invest Drugs 1996;5:207–23.
5. Waksman SA. Classification, identification and description of genera and species: The Actinomycetes. Vol. 2. Baltimore: Williams and Wilkins; 1961. p. 1–363.
6. Nino A Gagelidze, Lia L Amiranashvili, Tinatin A Sadunishvili, Giorgi I Kvesitadze, Tengiz F Urushadze, Tamar O Kvrivishvili. Bacterial composition of different types of soils of Georgia. Annals Agrarian Sci 2018;16:17-21.
7. Arifuzzaman M, Khatun MR, Rahman H. Isolation and screening of actinomycetes from sundarbans soil for antibacterial activity. Afr J Biotechnol 2011;9:4615-9.
8. Kurtboke DI, Chen CF, Williams ST. Use of polyvalent phage for reduction of streptomycetes on soil dilution plates. J Appl Bacteriol 1992;72:103–11.
9. Vineeta Singh, Shafiul Haque, Harshita Singh, Jyoti Verma, Kumari Vibha, Rajbir Singh, et al. Tripathi, isolation, screening, and identification of novel isolates of actinomycetes from India for antimicrobial applications. Front Microbiol 2016;7:19-21.
10. Pandey A, Nigam P, Soccol CR, Soccol VT. Advances in microbial amylases. Biotechnol Appl Biochem 2000;31:135-52.
11. Casida LE. Industrial microbiology. 3rd edition. Wiley Easter Ltd; 1984. p. 3-437.
12. James G. Cappuccinoand natalie sherman microbiology a laboratory manual. 10th edition. Pearson Education; 2014. p. 352-7.
13. Aneja KR. Experiments in microbiology, plant pathology and biotechnology, new age international private limited, Publishers, New delhi; 2010.
14. Bergeys manual of determinative bacteriology. 9th edition. The williams and Wilkins 428 East Preston street Baltimore, Maryland 21202, USA; 1994.
15. Berdy J. Recent advances in and prospects of antibiotic research. Process Biochem 1980;15:28-36.
16. Kori, MA Halkai. Pharmaceutical biotechnology fundamentals and applications; 2000. p. 140-60.
17. Sivaramakrishnan S. ?-amylases from microbial sources–an overview on recent developments. Food Technol Biotechnol 2006;44:173–84.
18. S Wang, C Lin, Y Liu, Z Shen, J Jeyaseelan, W Qin. Characterization of a starch-hydrolyzing ?-amylase produced by aspergillus niger WLB42 mutated by ethyl methanesulfonate treatment. Int J Biochem Mol Biol 2016;7:1-10.
19. Demain AL. Pharmaceutically active secondary metabolites of microorganisms. Appl Microbiol Biotechnol 1999;52:455-63.
20. Kathryn ER Davis, Shayne J Joseph, Peter H Janssen. Effects of the growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl Environ Microbiol 2005;71:826–34.
Statistics
22 Views | 9 Downloads
Citatons
How to Cite
SENTHILRAJR, G. S., T. M., S. A., L. V., and T. B. A. “SCREENING OF MULTI BIOACTIVE METABOLITES PRODUCING MICROORGANISMFROM GARDEN SOIL OF DR. M. G. R EDUCATIONAL AND RESEARCH INSTITUTE, CHENNAI”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 3, May 2020, pp. 87-90, doi:10.22159/ijcpr.2020v12i3.38313.
Section
Original Article(s)