STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS

  • DEEPAK SHRESTHA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • JITENDRA PANDEY Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • CHIRANJIBI GYAWALI Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • MAHESH LAMSAL Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • SUNITA SHARMA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • RABINDRA KUMAR ROKAYA Department of Pharmacy, Karnali Academy of Health Science, Jumla, Nepal
  • PRAMOD ARYAL Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
  • RAM BAHADUR KHADKA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • RAVIN BHANDARI Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal

Abstract

Objective: The systematic study of effective alternative anti-diabetic drugs has great importance to manage diabetes as well as other oxidative stress-related diseases. According to previous research, root and bark of Mussaenda macrophylla plant has anti-microbial, anti-coagulant, anti-inflammatory, and hepatoprotective activity. Ethnomedicinal data shows that Mussaenda macrophylla is used to treat diabetes as well as oxidative stress. The objective of this research is to investigate in vitro anti-diabetic and anti-oxidant activity of root extract of Mussaenda macrophylla.


Methods: DPPH free radical scavenging assay was used to detect anti-oxidant potency of ethanol and methanol root extract of the plant and expressed as % of radicle inhibition. Anti-diabetic activity was determined by the glucose diffusion method using a glucose oxidase kit and results were expressed as mean±SD.


Results: The ethanol root extract at the concentration of 50 mg/ml and 100 mg/ml showed better glucose diffusion inhibition than that of methanol extract at the same concentration on increasing time interval. Ethanol extract at the concentration 100 µg/ml displayed better DPPH scavenging activity (89.83±0.19 %) than that of methanol extract (86.61±0.75%).


Conclusion: This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model.

Keywords: Mussaenda macrophylla, Anti-diabetic, Anti-oxidant, In vitro, DPPH

References

1. Antolovich M, Prenzler PD, Patsalides E, Suzanne McDonald, Robards K. Methods for testing antioxidant activity. J Royal Soc Chem 2002;127:183-3.
2. Subba B, Basnet P. Antimicrobial and antioxidant activity of some indigenous plants of Nepal. J Pharmacogn Phytochem 2014;3:62-7.
3. Rajamanikandan S, Sindhu T, Durgapriya D, Sophia D, Ragavendran P, Gopalakrishnan VK. Radical scavenging and antioxidant activity of ethanolic extract of Mollugo nudicaulis by in vitro assays. Indian J Pharm Educ 2011;45:310-6.
4. Siju EN, Rajalakshmi GR, Joseph A. In vitro antioxidant activity of Mussaenda frondosa. Int J Pharm Tech Res 2010;2:1236-40.
5. Wang J, Cao X, Jiang H, Qi Y, Chin KL, Yue Y. Antioxidant activity of leaf extracts from different Hibiscus sabdariffa accessions and simultaneous determination of five major antioxidant compounds by LC-Q-TOF-MS. Molecules 2014;19:21226-38.
6. Vidyalakshmi KS, Vasanthi HR, Rajamanickam GV. Ethnobotany, phytochemistry and pharmacology of Mussaenda species (Rubiaceae). Ethnobotanical Leaflets 2008;12:469-5.
7. Mihailova S, Tsvetkvoa A. Pharmacological trends in the treatment of diabetes type 2-new classes of antidiabetic drugs. Int Arch Integr Med 2015;2:223-8.
8. Chowdhury SR, Akter S, Sharmin T, Islam F, Quadery TM. Antimicrobial activity of five medicinal plants of Bangladesh. J Pharmacogn Phytochem 2013;2:164-70.
9. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969;6:24-7.
10. Manandar NP. Plants and people of nepal. Timber Press Portland, Oregon, U. S. A; 2002. p. 69, 74, 75,157,214, 232, 234,326, 327, 382.
11. Islam F, Quadery TM, Chowdhury SR, Kaisar MA, Uddin G, Rashid MA. Antioxidant and cytotoxic activities of Mussaenda macrophylla. Bangladesh Pharm J 2012;15:69-71.
12. Gunasekaran S, Sundaramoorthy S, Sathiavelu M, Arunachalam S. The genus Mussaenda: a phytopharmacological review. J Chem Pharm 2015;7:1037-42.
13. Dinda B, Majumder S, Arima S, Sato N, Harigaya Y. Iridoid glucoside and sterol galactoside from Mussaenda macrophylla. J Nat Med 2008;62:447–51.
14. Kim NC, Desjardins AE, Wu CD, Kinghorn AD. Activity of triterpenoid glycosides from the root bark of Mussaenda macrophyll aagainst two oral pathogens. J Nat Prod 1999;62:1379-84.
15. Bhandari R, Sharma G, Jammarkatel Pandit N. Anti-oxidant activity of selected medicinal plants of the himalayan region. Int J Pharm Sci 2015;6:473-7.
16. Priyadarshini SS, Vadivu R, Jayshreee N. Hypolipidemic and renoprotective study on the ethanolicand aqueous extracts of leaves of Ravenalamadagascariensis sonn. onalloxan induced diabetic rats. Int J Pharm Sci 2010;2:44-50.
17. Gallagher AM, Flatt PR, Duffy, Abdel Wahab YHA. The effects of traditional anti diabetic plants on in vitro glucose diffusion. Nutr Res 2003;23:413-24.
18. Edwards CA, Black burn NA, Craigne L, Daavidson P, Tomlin J, Sugden K, et al. Viscosity of food gums determined in vitro related to their hypoglycemic actions. Am J Clin Nutr 1987;46:72-7.
19. Subba B, Basnet P. Antimicrobial and antioxidant activity of some indigenous plants of Nepal. J Pharmacogn Phytochem 2014;3:62-7.
20. Singab AN, Youssef FS, Shour AL. Medicinal plants with potential antidiabetic activity and their assessment. Med Aromat Plants 2014;3:1-12.
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SHRESTHA, D., J. PANDEY, C. GYAWALI, M. LAMSAL, S. SHARMA, R. K. ROKAYA, P. ARYAL, R. B. KHADKA, and R. BHANDARI. “STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 4, July 2020, pp. 70-74, doi:10.22159/ijcpr.2020v12i4.39085.
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