IN VITRO ANTIDIABETIC POTENTIALS OF SIDA ACUTA, ABUTILON INDICUM AND MALVASTRUM COROMANDELIANUM

  • NANTAPORN DINLAKANONT College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • CHANIDA PALANUVEJ College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • NIJSIRI RUANGRUNGSI College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand, College of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand

Abstract

Objective: Starch metabolizing enzyme inhibitors are able to retard postprandial glucose absorption. This study aimed to investigate the in vitro inhibitory activities of alpha-glucosidase and alpha-amylase of three Malvaceous weeds i.e. Sidaacuta Burm. f., Abutilon indicum (Linn.) Sweet and Malvastrumcoromandelianum (Linn.) Garcke.


Methods: The stems, roots and leaves of S. acuta, A. indicum and M. coromandelianum were sequentially extracted in dichloromethane and methanol, respectively. All fractions were tested for the inhibitory activities on yeast alpha-glucosidase, rat intestinal alpha-glucosidase and porcine alpha-amylase. p-Nitrophenyl-α-D-glucopyranoside and 2-chloro-4 nitrophenol-α-D- maltotrioside were used as the substrate for glucosidase and amylase respectively.


Results: The dichloromethane fraction of the roots and stems from A. indicum and dichloromethane as well as methanolic fractions of the stems of M. coromandelianum could inhibit yeast alpha-glucosidase compared to 1-deoxynojirimycin with the IC50 of 0.36, 0.45, 0.48, 0.48 and 0.58 mg/ml respectively. A. indicum root methanolic fraction had the highest inhibitory effect on rat alpha-glucosidase activity compared to 1-deoxynojirimycin with the IC50 of 0.08 and 0.11 mg/ml respectively. M. coromandelianum, the dichloromethane fraction of roots and the methanolic fraction of stems, showed the strongest effect on alpha-amylase inhibition compared to acarbose with the IC50 of 0.07, 0.07 and 2.7 mg/ml, respectively.


Conclusion: S. acuta, A. indicum and M. coromandelianum dichloromethane and methanolic fractions of the root, stem and leaf parts demonstrated an appreciable inhibitory activity on alpha-amylase from porcine, alpha-glucosidase from Saccharomyces cerevisiae and from rat intestine compared to 1-deoxynojirimycin and acarbose.

Keywords: Alpha-amylase, Alpha-glucosidase, Enzyme inhibition, Malvaceous weed

References

1. Gray GM. Starch digestion and absorption in nonruminants. J Ntur 1992;122:172-7.
2. Dinda B, Das N, Dinda S, Dinda M, Silsarma I. The genus Sida L.-A traditional medicine: its ethnopharmacological, phytochemical and pharmacological data for commercial exploitation in herbal drugs industry. J Ethnopharmacol 2015;176:135-76.
3. Arya A, Abdullah MA, Haerian BS, Mohd MA. Screening for hypoglycemic activity on the leaf extracts of nine medicinal plants: in vivo evaluation. J Chem 2012;9:1196-205.
4. Okwuosa CN, Azubike NC, Nebo II. Evaluation of the anti-hyperglycaemic activity of crude leaf extracts Sidaacuta in normal and diabetic rabbits. Indian J Novel Drug Delivery 2011;3:206–13.
5. Krisanapun C, Lee SH, Peungvicha P, Temsiririrkkul R, Baek SJ. Antidiabetic activities of Abutilon indicum (L.) sweet are mediated by enhancement of adipocyte differentiation and activation of the GLUT1 promoter. Evid Based Complement Alternat Med 2011. DOI:10.1093/ecam/neq004
6. Peungvicha P, Krisanapun C, Temsiririrkkul R, Wongkrajang Y. Aqueous extract of Abutilon indicum sweet inhibits glucose absorption and stimulates insulin secretion in rodents. Nutr Res 2009;29:579-87.
7. Deore AB, Chavan PN, Sapakal VD, Naikwade NS. Antidiabetic and antihyperlipidemic activities of Malvastrumcoromandelianum linn leaves in alloxan-induced diabetic rats. Int J Pharmtech Res 2012;4:351-7.
8. Arciniegas A, Perez Castorena AL, Nieto Camacho A, Kita Y, Vivar AR. Anti-hyperglycemic, antioxidant, and anti-inflammatory activities of extracts and metabolites from Sidaacuta and Sidarhombifolia. Quimica Nova 2017;40:176-81.
9. Aderogba MA, Madikizela B, Mcgaw LJ. Bioactive constituents from Malvastrumcoromandelianum (L.) garcke leaf extracts. S Afr J Bot 2019;126:371-6.
10. Mengting Ni, Xing Hu, Deming Gong, Guowen Zhang. Inhibitory mechanism of vitexin on ?-glucosidase and its synergy with acarbose. Food Hydrocoll 2020;105:105824.
Statistics
50 Views | 53 Downloads
Citatons
How to Cite
DINLAKANONT, N., C. PALANUVEJ, and N. RUANGRUNGSI. “IN VITRO ANTIDIABETIC POTENTIALS OF SIDA ACUTA, ABUTILON INDICUM AND MALVASTRUM COROMANDELIANUM”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 4, July 2020, pp. 87-89, doi:10.22159/ijcpr.2020v12i4.39088.
Section
Original Article(s)