• NATARAJAN K. Department of Pharmaceutical Biotechnology, T John College of Pharmacy, Bengaluru
  • KUMARESH JAWAHAR N. Department of Biotechnology, SASTRA University, Tanjore


Objective: To study the efflux pump inhibition activity of Mucunacochinchinensis extracts

Methods: Sensitivity assay was performed by Kirby Bauer technique. Effluxing ability of microbe was done by accretion, accumulation and efflux pump assays.

Results: Resistant fungal strains were subjected to efflux ability studies. Maximum effluxing ability was determined from the experimental data and it was found to be 105 min and 75 min by Candida albicans for methanolic extract of M. cochinchinenesis (MMC) and ethanolic extract of M. cochinchinensis (EMC) respectively and 45 min and 105 min by Aspergillus niger for MMC and EMC respectively. Profound synergistic effect of inhibition was observed with combined MMC and standard Clotrimoxazole when compared to the standard alone; against C. albicansindicates the modulating efficacy of MMC.

Conclusion: M. cochinchinensis extracts showed efflux pump modulating activity.

Keywords: Mucunacochinchinensis, Efflux pump, Fungal strains


1. Melkamu Abebe, Senait Tadesse, Girum Meseret, Awoke Derbie. Type of bacterial isolates and antimicrobial resistance profile from different clinical samples at a referral hospital, Northwest Ethiopia: five years data analysis. BMC Res Notes 2019;12:1-6.
2. Denise E Morris, David W Cleary, Stuart C Clarke. Secondary bacterial infections associated with influenza pandemics. Front Microbiol 2017;8:1041.
3. Evans WC. Trease and evans pharmacognosy. 15th edition. WB Saunders Edinburgh, London; 2002. p. 523.
4. Khory RN, Katrat NN. Materia medica of India and their therapeutics. Komal Prakashan, Delhi; 1999. p. 218-9.
5. Rajeshwar Yerra, Malaya Gupta, Upal Kanti Mazumder. In vitro lipid peroxidation and antimicrobial activity of Mucunapruriens seeds. Iran J Pharmacol Ther 2005a;4:32-5.
6. Manjunatha BK, Patil HSR, Vidya SM, Kekuda TRP, Mukunda S, Divakar R. Studies on the antibacterial activity of Mucunamonosperma DC. Indian Drugs 2006;43:150-2.
7. Harborne JB. Phytochemical methods. 3rd edition. Springer (India) Pvt Ltd, New Delhi; 2005. p. 75-89.
8. Patrick J Sinko. Martins physical pharmacy and pharmaceutical sciences. 6th edition. Wolters Kluwer (India) Pvt Ltd, New Delhi; 2010. p. 80-4.
9. Lazaroaie MM. Mechanisms involved in organic solvent resistance in gram-negative bacteria world academy of sciences. Eng Technol 2009;54:648-58.
10. Edward A, Melchias G, Visvanathan D, Prashanth Kumar Soni, Ajithan C. Role of efflux pumps in affording organic solvent tolerance to bacterial strains from petroleum contaminated soil. Middle East J Sci Res 2012;12:80-7.
11. Zgurskaya HI, Nikaido H. Multidrug resistance mechanisms: drug efflux across two membranes. Mol Microbiol 2000;37:219-25.
12. Horobin RW, Kiernan JA. Conn's biological stains. 10th edn. Oxford, UK: BIOS Scientific Publishers; 2002.
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How to Cite
K., N., and K. J. N. “EFFECT OF MUCUNA COCHINCHINENSIS EXTRACTS ON EFFLUX PUMP IN FUNGAL STRAINS”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 4, July 2020, pp. 94-96, doi:10.22159/ijcpr.2020v12i4.39090.
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