FORMULATION AND EVALUATION OF ANTI-ACNE GEL CONTAINING MURRAYA KOEINIGII EXTRACT
Keywords:Acne vulgaris, Murraya koeinigii, Propionibacterium acne
Objective: Acne, one among the very fashionable socially distressing skin conditions created by Propionibacterium acne have generally been treated by antibiotics. Within the light of the growing threat of antibiotic resistance, natural plant products are applied as a safer alternative. Keeping the very fact in the background, during this research work, the formulation of gel from the extracts of Murraya koeinigii leaves are prepared and evaluated as an anti-acne drug.
Methods: The fresh leaf extracts were subjected to phytochemical and antimicrobial screening. Minimum Inhibitory Concentration (MIC) decided. Gel formulation of the extracts was developed and evaluated. The manufactured formulations were subjected to In vitro antibacterial activity against P. acnes, S. epidermidis and S. aureus. The marker compound, clindamycin, in herbal anti-acne preparation, was kept for the comparison with the zones of inhibition for antibacterial activity.
Results: Anti-acne property was explored with the help of a standard curve and by comparing diffusion profiles by taking clindamycin as a reference.
Conclusion: From the present study it can be concluded that addition of permeation enhancer in the test formulation will improve the diffusion profile and thus it was designed to add permeation enhancer.
2. Rocha MA, Bagatin E. Skin barrier and microbiome in acne. Arch Dermatol Res 2018;310:181–5.
3. Chilicka K, Maj J, Panaszek B. General quality of life of patients with acne vulgaris before and after performing selected cosmetological treatments. Patient Prefer Adherence 2017;11:1357–61.
4. Gollnick HPM, Dreno B. Pathophysiology and management of acne. J Eur Acad Dermatol Venereol 2015;29:1–2.
5. Adler BL, Kornmehl H, Armstrong AW. Antibiotic resistance in acne treatment. JAMA Dermato 2017;153:810–1.
6. Mandal SK. Indanyl analogs as potential antimicrobial agents. Asian J Pharm Clin Res 2018;11:278-80.
7. Nasri H, Bahmani M, Shahinfard N, Nafchi AM, Saberianpour S, Kopaei MR. Medicinal plants for the treatment of acne vulgaris: a review of recent evidences. Jundishapur J Microbiol 2015;8:11.
8. Shekar BC, Nagarajappa R, Jain R, Singh R, Suma S, Thakur R. Antimicrobial efficacy of acacia nilotica, murraya koenigii L. sprengel, eucalyptus hybrid, psidium guajava extracts and their combinations on Fusobacterium nucleatum and porphyromonas gingivalis. Indian J Dent Res 2018;29:641–5.
9. Yadav S, Vats V, Dhunnoo Y, Grover JK. Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats. J Ethnopharmacol 2002;82:111–6.
10. Samanta SK, Kandimalla R, Gogoi B, Dutta KN, Choudhury P, Deb PK, et al. Phytochemical portfolio and anticancer activity of murraya koenigii and its primary active component, mahanine. Pharmacol Res 2018;129:227–36.
11. Rajput M, Khan RA. Phytochemical screening, acute toxicity, anxiolytic and antidepressant activities of the Nelumbo nucifera fruit. Metab Brain Dis 2017;32:743–9.
12. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016;6:71–9.
13. Ng SF, Rouse J, Sanderson D, Eccleston GA. Comparative study of transmembrane diffusion and permeation of ibuprofen across synthetic membranes using franz diffusion cells. Pharmaceutics 2010;2:209–23.
14. Melnik BC, Schmitz G, Zouboulis CC. Anti-acne agents attenuate FGFR2 signal transduction in acne. J Invest Dermatol 2009;129:1868–77.
15. Rahman MM, Gray AI. A benzoisofuranone derivative and carbazole alkaloids from Murraya koenigii and their antimicrobial activity. Phytochemistry 2005;66:1601–6.