• JOHNY MARPAUNG Department of Obstetric and Gynaecology, Medical Faculty, Universitas Sumatera Utara, General Hospital H. Adam Malik Medan
  • M. F. G. SIREGAR Fertility Endocrinology Division, President of Persatuan Menopause Indonesia (PERMI), Department of Obstetric and Gynaecology, Medical Faculty, Universitas Sumatera Utara, General Hospital H. Adam Malik Medan
  • MAKMUR SITEPU Department of Obstetric and Gynaecology, Medical Faculty, Universitas Sumatera Utara, General Hospital H. Adam Malik Medan
  • ADANG BACHTIAR Public Health Department, Medical Faculty, Universitas Indonesia



Black cumin, Systolic, Diastolic, Mean arterial pressure, Proteinuria


Objective: This research aimed to show effect of black cumin (nigella sativa) on blood pressure, mean arterial pressure (MAP), proteinuria in preeclamptic model rats.

Methods: This is analytical research with true experimental design in laboratory pregnant female rats (Rattus norvegicus), which get black cumin seed extract (Nigella sativa) at a dose of 500 mg/kg/day and 2000 mg/kg/day. Treatment of all samples was performed simultaneously and during the treatment was observed using Postest Only Control Group Design. The research was conducted at Biology Laboratory in July 2019. To assess the comparison of parameters (systolic and diastolic blood pressure, mean arterial pressure and proteinuria) between groups the ANOVA test was used if the data were normally distributed and Kruskal Wallis test was used if the data were abnormally distributed.

Results: Systolic and diastolic blood pressure and MAP decreased in preeclampsia models rats by administering 500 mg (P1) and 2000 mg (cumin) black cumin extract (P2). However, a dose of 2000 mg black cumin extract had a more significant decrease in systolic blood pressure and MAP. The results of this research indicate that all treatment groups showed improvement after day 9 of the administration of nigella that no treatment group showed proteinuria.

Conclusion: Black cumin is proven to reduce systolic and diastolic blood pressure, Mean Arterial Pressure and proteinuria.


Download data is not yet available.


1. Kementerian Kesehatan Republik Indonesia. Survei Demografi dan Kesehatan Indonesia. Pusat Data dan Informasi Kementerian Kesehatan Republik Indonesia; 2018.
2. Achadi EL. Kematian maternal dan neonatal di Indonesia. FKM UI; 2019.
3. Amaral LM, Cunningham MW, Cornelius DC, LaMarca B. Preeclampsia: long-term consequences for vascular health, Vasc. Health Risk Manag 2015;11:403–15.
4. World Health Organization. WHO recommendations for prevention and treatment of pre-eclampsia and eclampsia; 2011.
5. Manik IN, Sari RDP, Wulan AJ. Hubungan status preeklampsia dengan kejadian perdarahan postpartum pada ibu bersalin di RSUD dr h abdul moeloek provinsi lampung periode 1 Jul 2014-30 Jun 2015. J Majority 2017;6:51-7.
6. Doshi SB, Agarwal A. The role of oxidative stress in menopause. J Midlife Health 2013;4:140-6.
7. MFG Siregar, Siburian PS, Kaban YB, Siregar HS, Effendi IH, Rivani R. The differences of catalase enzyme level between menopause andreproductive women. Giorn It Ost Gin 2015;XXXVII:6.
8. Bahareh A, Hossein H. Black cumin (Nigella sativa) and its ac-tive constituent, thymoquinone: an overview on the analgesic and anti-inflammatory effects. Planta Med 2016;82:8-16
9. Shahraki S, Khajavirad A, Shafei MN, Mahmoudi M, Tabasi NS. Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines. J Tradit Complement Med 2016;6:89-96.
10. Gong P, Liu M, Hong G, Li Y, Xue P, Zheng M, et al. Curcumin improves LPS-induced preeclampsia-like phenotype in rat by inhibiting the TLR4 signaling pathway. Placenta 2016;41:45-52.
11. Pennington KA, Schlitt JM, Jackson DL, Schulz LC, Schust DJ. Preeclampsia: multiple approachs for a multifactorial disease. Dis Model Mech 2012;5:9-18.
12. Leong XF, Rais Mustafa M, Jaarin K. Nigella sativa and its protective role in oxidative stress and hypertension. Evid Based Complementary Altern Med 2013.
13. Jaarin K, Foong WD, Yeoh MH, Kamarul ZYN, Qodriyah HMS. Mechanism of the anti-hypertensive effect of Nigella sativa oil in L-NAME induced hypertensive rats. Clinics 2015;70:751–7.
14. Alagawany M, Farag MR, Tiwari R, Karthik K, Dhama K. Nutritional, healthical and therapeutic efficacy of black cumin (Nigella sativa) in animals, poultry and humans. Int J Pharmacol 2016;12:232-48.
15. El-Sherbiny SS, El-Tamany ESH, El-Maksoud A. Protective effect of allium sativum and nigella sativa against no-mediated alterations in dimethylhydrazine-induced colon cancer rats. Egypt J Hosp Med 2004;15:1-14.
16. Adeneye A, Eddouks M. Phytotherapy in the management of diabetes and hypertension. In book: Phytotherapy of hypertension and diabetes mellitus. ed. 1st edition. Bentham Science Publishers; 2016. p. 131-63.
17. Hebi M, Zeggwagh N, Hajj L, El Bouhali B, Eddouks M. Cardiovascular effect of Nigella sativa L. aqueous extract in normal rats. Cardiovascular and Haematological Disorders Drug Targets 2016;16:47-55.
18. Peixoto Neves D, Silva Alves KS, Gomes MDM, Lahlou S, Magalhaes PJ. Vasorelaxant effects of the monoterpenic phenol isomers, carvacrol and thymol, on rat isolated aorta. Fundamental Clin Pharmacol 2010;24:341-50.
19. Sahebkar A, Soranna D, Liu X, Thomopoulos C, Simental Mendia LE, et al. A systematic review and meta-analysis of randomized controlled trials investigating the effects of supplementation with Nigella sativa (black seed) on blood pressure. J Hypertens 2016;34:2127-35.
20. Rahma H, Indrawan IWA, Nooryanto M. Effect of a black cumin (Nigella sativa) ethanol extract on placental angiotensin II type 1-receptor autoantibody (AT1-AA) serum levels and endothelin-1 (ET-1) expression in a preeclampsia mouse model. J Taibah Univ Med Sci 2017;12:528.
21. Yimer EM, Tuem KB, Karim A, Ur Rehman N, Anwar F. Nigella sativa L. (black cumin): a promising natural remedy for wide range of illnesses. Evid Based Complementary Altern Med 2019;54:671-8.
22. Zainiyah H, Noviani NW, Hipni R. The positive efect of black cumin ethanol extract (Nigella sativa) on decreasing serum TNF-alpha levels and interleukin-8 in mice of preeclampsia. Biomed Res 2018;29:3801-6.
23. Badary OA, Abdel Naim AB, Abdel Wahab MH, Hamada FM. The influence of thymoquinone on doxorubicin-induced hyperlipidemic nephropathy in rats. Toxicology 2000;143:219-26.



How to Cite

MARPAUNG, J., M. F. G. SIREGAR, M. SITEPU, and A. BACHTIAR. “BLACK CUMIN (NIGELLA SATIVA) EFFECT ON BLOOD PRESSURE, MEAN ARTERIAL PRESSURE, PROTEINURIA IN PREECLAMPTIC MODEL RATS”. International Journal of Current Pharmaceutical Research, vol. 12, no. 4, July 2020, pp. 127-33, doi:10.22159/ijcpr.2020v12i4.39099.



Original Article(s)