PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES
Keywords:Oxidative stress, Polyphenolics, Flavonoids, Inflammation, Medicinal plants, Herbal medicine
Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf.
Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated.
Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays.
Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.
2. Yamagishi S, Matsui T. Nitric oxide, a Janus-faced therapeutic target for diabetic microangiopathy-friend or foe? Pharmacol Res 2011;64:187-94.
3. Anokwuru CP, Esiaba I, Ajibaye O, Adesuyi AO. Polyphenolic content and antioxidant activity of Hibiscus sabdariffa calyx. Res J Med Plant 2011;5:557-66.
4. Mbaebe BO, Edeoga HO, Afolayan AJ. Phytochemical analysis and antioxidants activities of aqueous stem bark extract of Schotia latifolia Jacq. Asian Pac J Trop Biomed 2012;2:118-24.
5. Meenakshi S, Umayaparvathi S, Arumugam M, Balasubramanian T. In vitro antioxidant properties of FTIR analysis of two sea weeds of gulf of mannar. Asian Pac J Trop Biomed 2011;1:S66-S70.
6. Trease G, Evans SM. Pharmacognosy. 15th ed. London: Bailer Tindal; 2002. p. 23-67.
7. Harborne JB. Phytochemical methods-a guide to modern techniques of plant analysis. 2nd ed. London: Chapman and Hall; 1984. p. 14-6.
8. Tirzitis P, Bartosz G. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochimica Polonica 2010;57:139-42.
9. Sudha M, Indhushree R, Vadivukkarasi M. In vitro free radical scavenging activity of raw pepino fruit (solanum muricatum aiton). Int J Curr Pharm Res 2011;3:98-110.
10. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337–41.
11. Goyal AK, Mishra T, Bhattacharya M. Evaluation of phytochemical constituents and antioxidant activity of selected actinorhizal fruits growing in the forests of Northeast India. J Biosci 2013;38:797-803.
12. Torres AM, Mau Lastovicka T, Rezaaiyan R. Total phenolics and high performance liquid chromatography of phenolic acids of avocado. J Agric Food Chem 1987;35:921-5.
13. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavanoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
14. Vishnu R, Nisha R, Jamuna S, Paulsamy S. Quantification of total phenolics and flavonoids and evaluation of in vitro antioxidant properties of methanolic leaf extract of Tarenna asiatica-an endemic medicinal plant species of Maruthamali hills, Western Ghats, Tami Nadu. J Res Plant Sci 2013;2:196–204.
15. Benedec D, Vlase L, Oniga I, Mot AC, Damian G, Hanganu D. Polyphenolic composition, antioxidant and antibacterial activities for two romanian subspecies of Achillea distans waldst et kit ex wild. Molecules 2013;18:8725–39.
16. Charalampos P, Konstantina L, Olga KM, Panagiotis Z, Vassileia JS. Antioxidant capacity of selected plant extracts and their essential oils. Antioxidants 2013;2:11–22.
17. Paulsamy S, Jeeshna MV. Preliminary phytochemistry and antimicrobial studies of an endangered medicinal herb exacum bicolor roxb. Res J Pharm Biol Chem Sci 2011;2:447–57.
18. Geetha S, Sai Ram M, Mongia SS, Singh V, Ilavazhagan G. Evaluation of antioxidant activity of leaf extract of sea buckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J Ethnopharmacol 2003;87:247-51.