• PAYEL SUTRADHAR Department of Microbiology, Institute of Genetic Engineering. Kolkata, West Bengal, India
  • SHALINI GHOSH Department of Microbiology, Institute of Genetic Engineering. Kolkata, West Bengal, India
  • BINITA KUMARI ROY Department of Microbiology, Institute of Genetic Engineering. Kolkata, West Bengal, India




Vitamin C, DCPIP, Emblica officinalis (amla), Antimicrobial efficacy, E coli, Zone of inhibition, Microwave, Boiling


Objective: The present work aimed to expand the awareness of restoring vitamin-C in its active form on different heat exposures. The effect of microwave-assisted processing and boiling of the aqueous crude extract of citrus fruit Emblica officinalis (amla) has been correlated with its antimicrobial efficacy against E. coli.

Methods: The aqueous crude extract of dried amla pulp exposed to microwave radiation(600W,5 min) and boiling (5 min) were titrimetrically estimated for vitamin-C content by DCPIP-(2,6, Dinitrophenol indophenol) method and compared the same with the untreated sample. These three samples were studied for their effect on the growth pattern of E. coli turbidimetrically. The antimicrobial susceptibility test by agar cup well diffusion method was further followed to measure the zone of inhibitions (ZOI) for these three test extracts against E. coli.

Results: The total estimated vitamin-C content was 26.76 mg/100g, 25.35 mg/100g and 21.12 mg/100g in the untreated extract (UTE), microwaved extract (MWE) and boiled extract (BE) respectively. At a higher concentration (0.8 mg/ml), the UTE showed a greater ZOI of 20 mm and a comparable ZOI of 18 mm for the MWE against E. coli. In addition, a reduced ZOI of 10 mm was recorded in case of the BE. At a lowest concentration (0.05 mg/ml), the UTE inhibited the growth with a least ZOI of 7 mm, whereas no inhibition zones were detected for MWE and BE at this concentration.

Conclusion: The present investigation demonstrated the effect of boiling and microwave-assisted processing on the content of bioactive vitamin-C and its antimicrobial activity. The DCPIP method calculated a more vitamin-C retention in the MWE than the BE. As the boiling method destroyed the vitamin more rapidly, a higher growth rate of E. coli was measured in the presence of BE than the UTE and MWE. In addition, the antimicrobial assay also showed a least inhibitory effect against E. coli in the presence of the BE. A moderate inhibitory effect for MWE was also detected. Thus the present investigation proved that the boiling process destroys vitamin-C present in a food sample to a higher extent than the microwave-assisted processing.


Download data is not yet available.


1. Verghese RJ, Mathew SK, David A. Antimicrobial activity of vitamin C demonstrated on uropathogenic Escherichia coli and Klebsiella pneumoniae. J Curr Res Sci Med 2017;3:88-93.
2. Sudha K, Baskaran D, Dhanalakshmi B, Pugazhenthi TR. Determination of the antimicrobial activity of fruits. Int J Med Pharm Sci 2017;7:1-8.
3. Delazar A, Nahar L, Hamedeyazdan S, Sarker Sd. Microwave-assisted extraction in natural products isolation. Methods Mol Biol 2012;864:89-115.
4. Hui Min Zhang, Noriko Wakisaka MS, Osamu Maeda MS, Tatsuo Yamamoto. Vitamin C inhibits the growth of a bacterial risk factor for gastric carcinoma: Helicobacter pylori. Am Cancer Soc 2000;80:1897-903.
5. Sadashivam S, Theymoli Balasubramanian. In: Practical Manual in Biochemistry Tamil Nadu Agricultural University, Coimbatore; 1987.
6. Cappuccino James G, Sherman Natalie. In: Microbiology: A laboratory manual: 10th edition. SUNY Rockland Community College; 2014.
7. Geller, Bruce, Deere, Jesse, Tilley. Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis. J Antimicrobial Chemother 2005;55:983-8.
8. Igwemmar NC, Kolawole SA, Imran IA. Effect of heating on vitamin c content of some selected vegetables. Int J Sci Technol Res 2013;2:209-12.
9. Caputo L, Quintieri L, Cavalluzzi MM, Lentini G, Habtemariam S. Antimicrobial and antibiofilm activities of citrus water-extracts obtained by microwave-assisted and conventional methods. Biomedicines 2018;6:70.
10. Proestos Charalampos, Komaitis Michael. Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. Food Sci Technol 2008;41:652-9.
11. Rumm Kreuter D, Demmel I. Comparison of vitamin losses in vegetables due to various cooking methods. J Nutr Sci Vitaminol 1990;36 Suppl 1:S7-14, S14-5.



How to Cite

SUTRADHAR, P., S. GHOSH, and B. K. ROY. “EFFECT OF BOILING AND MICROWAVE ASSISTED PROCESSING ON THE ANTIMICROBIAL EFFICACY OF VITAMIN–C IN EMBLICA OFFICINALIS”. International Journal of Current Pharmaceutical Research, vol. 12, no. 5, Sept. 2020, pp. 102-5, doi:10.22159/ijcpr.2020v12i5.39780.



Original Article(s)