BIODEGRADABLE PLASTIC (PHB) FROM BACTERIA COLLECTED FROM GOPALPUR BEACH, BHUBANESWAR
Objective: Environmental concerns have prompted research into the development of biodegradable polymer as an alternative to petroleum-based plastics. Polyhydroxybutyrates (PHBs) are good alternatives to produce biodegradable plastics. Some bacteria are found to be producing PHB. The aim of this work was to isolate potential PHB producing bacteria and screen them for their ability to produce PHB.
Methods: Contaminated water sample from Gopalpur beach, Bhubaneswar was the source of bacteria. Three colonies were isolated from the water sample. The samples were named as C1, C2 and C3. The colonies were first identified by colony morphology. The sudan black screening test was done to screen for the production of (PHB) polyhydroxy butyrate by bacterial isolates.
Results: The bacterial isolates C1 and C2 showed a positive result for the production of polyhydroxy butyrate (PHB). Presence of PHB granules in Cocobacillus and Rod shaped bacillus was confirmed.
Conclusion: Polyhydroxybutyrate (PHB), a kind of microbial polyester that accumulates as a carbon/energy reserve material in various microorganisms, was thus concluded to be a decent alternative for plastics. Because of their special characteristics and broad biological applications, biodegradable plastics are compounds with a promising future.
2. BrandI H, Richard A Gross, Robert W Lenz, R Clinton Fuller. Plastics from bacteria and for bacteria: poly(?-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters from. Adv Biochem Eng Biotechnol 1990;41:77-93.
3. Anon Thammasittirong, Sudarat Saechow, Sutticha Na-Ranong Thammasittirong. Efficient polyhydroxybutyrate production from Bacillus juice substrate thuringiensis using sugarcane. Turk J Biol 2017;41:992–1002.
4. Alyaa Hamieh, Zakia Olama, Hanafi Holail. Microbial production of polyhydroxybutyrate, biodegradable plastic using agro-industrial waste products. Global Adv Res J Microbiol 2013;2:54-64.
5. Neha Sharma. PHB production by bacteria and it’s application as biodegradable plastic in various industries. Academic J Polymer Sci 2019;2:2641-8282.
6. C Pena, T Castillo, A Garcia, M Millan, D Segura. Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microbial Biotechnol 2014;7:278–93.
7. KR Shah. Optimization and production of polyhydroxybutarate(PHB) by bacillus subtilis G1S1 from soil. Int J Curr Microbiol Appl Sci 2014;3:377-87.
8. Christina Thapa, Pallavi Shakya, Rabina Shrestha, Sushovita Pal, Prakash Manandhar. Isolation of polyhydroxybutyrate (PHB) producing bacteria, optimization of culture conditions for PHB production, extraction and characterization of PHB. Nepal J Biotechnol 2018;6:62-8.
9. Jacquel Nicolas, Chi Wei Lo, Ho Shing Wu, Yu Hong Wei, Shaw S. Wang solubility of polyhydroxyalkanoates by experiment and thermodynamic correlations. AIChE J 2007;53:2704-14.
10. Sabbir Ansari, Tasneem Fatma. Cyanobacterial polyhydroxybutyrate (PHB): screening, optimization and characterization. PLoS One 2016;11:e0158168.
11. Jikasmita Dalei, Debasish Sahoo, Aishna Sagar. Isolation, characterization of poly-hydroxy butyrate (PHB) producing bacteria from paddy soil and contaminated lake water and comparative study for productivity of PHB from different isolates. IJESR 2015;3:2347-6532.
12. Jose M Luengo, Belen Garcia, Angel Sandoval, German Naharro, Elias R Olivera. Bioplastics from microorganisms. Curr Opinion Microbiol 2003;6:251-60.
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