“MYCO SYNTHESIS OF SILVER NANOPARTICLES USING GLIOCLADIUM ROSEUM (CLONOSTACHYS ROSEA (LINK) SCHROERS, SAMUELS) AND ITS ANTIMICROBIAL EFFICACY AGAINST SELECTED PATHOGENS”

  • BHARATH NAIR Department of Forest Pathology, Kerala Forest Research Institute, Peechi 680653, Thrissur, Kerala
  • DELMY ABRAHAM Department of Forest Pathology, Kerala Forest Research Institute, Peechi 680653, Thrissur, Kerala
  • AMRITA DINESH Department of Forest Pathology, Kerala Forest Research Institute, Peechi 680653, Thrissur, Kerala
  • G. E. MALLIKARJUNA SWAMY Department of Forest Pathology, Kerala Forest Research Institute, Peechi 680653, Thrissur, Kerala

Abstract

Objective: Antimicrobial efficacy of silver nanoparticles from Gliocladium roseum, culture filtrate (C. F.) and mycelial mat extract (M. E.) against selected pathogens.


Methods: Culture filtrate (C. F.) and Mycelial mat extract (M. E.) of Gliocladium roseum were subjected to 10 Mm silver nitrate solution for the synthesis of silver nanoparticles. Formed silver nanoparticles were evaluated via UV-vis spectroscopy and the structural elucidation was done by FT-IR and TEM. Antimicrobial efficacy was tested against bacterial (Salmonella typhi and Klebsiella pneumonia) and fungal (Cladosporium cladosporioides and Alternaria alternata) pathogens. Different nanoparticle concentrations-50, 100, 150 and 200 µl were checked via disc diffusion method.


Results: Gliocladium roseum (C. F. and M. E.) on interaction with silver nitrate solution effectively reduced metallic silver exhibiting a colour change from yellow to dark brown within 24 h due to the formation of silver nanoparticles. The UV-vis spectrum of C. F. and M. E. showed maximum absorption peaks at 350-400 nm and 400-450 nm respectively and FT-IR and TEM showed strong N-H bonding and spherical shaped silver nanoparticles with the size of 11-19 nm (C. F.) and 25-38 nm (M. E.). Antimicrobial analysis resulted in efficient inhibitory activity against Salmonella typhi, Klebsiella pneumonia and also showed moderate inhibitory activity against Alternaria alternata and Cladosporium cladopsorioides.


Conclusion: The synthesis of silver nanoparticles from fungus Gliocladium roseum is simple, cheap, safe and eco-friendly thus emphasising on large scale scientific application.

Keywords: Gliocladium roseum, Silver nanoparticles, Culture filtrate (C. F.), Mycelial mat extract (M. E.), Antimicrobial activity

References

1. Jones SA, Bowler PG, Walker M, Parsons D. Controlling wound bioburden with a novel silver-containing Hydrofiber dressing. Wound Repair Regen 2004;12:288-94.
2. Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomed Nanotechnol 2007;3:168-71.
3. Silva Paula MMD, Franco CV, Cesar BM, Rodrigues L, Barichello T, Savi GD, et al. Synthesis, characterization and antibacterial activity studies of poly-{styrene-acrylic acid} with silver nanoparticles. Mater Sci Eng 2009;29:647-50.
4. Pinto RJB, Marques PAAP, Neto CP, Trindade, T, Daina S, Sadocco P. Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomate 2009;5:2279-89.
5. Koushik M, Ashtaputre S, Vogel W, Urban J, Kulkarni SK, Paknikar KM. Extracellular biosynthesis of nanoparticles by sliver tolerant yeast strain MKY3. Nanotechnology 2003;14:95-100.
6. Evanoff I, Salopek SB. Silver nanoparticles as antimicrobial agent. A case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci 2004;275:A177–182.
7. Kim JY, Sungeun K, Kim J, Jongchan L, Yoon J. The biocidal activity of nano-sized silver particles comparing with silver ion. Korean Soc. Environ Eng 2005;27:771-6.
8. Chen X, Schluesener HJ. Nanosilver: a nanoproduct in medical application. Toxicol Lett 2008;176:1-12.
9. Kim JSE, Kuk KN, Yu JH, Kim SJ, Park HJ. Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol 2007;3:95-101.
10. Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, et al. Fungus mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett 2001;1:515–9.
11. Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. Academic Press, London; 1980.
12. Mohan YM, Raju KM, Sambasivudu K, Singh S, Sreedhar B. Preparation of acacia-stabilized silver nanoparticles: a green approach. J Appl Polym Sci 2007;106:3375–81.
13. Moody AR, Gindrat D. Biological control of cucumber black root rot by Gliocladium roseum. Phytopathology 1977;67:1159-62.
14. Pratella GC, Mari M. Effectiveness of Trichoderma, Gliocladium, and Paecilomyces in postharvest fruit protection. Postharvest Biol Technol 1993;3:49-56.
15. Seifert KA, Breuil C, Rossignol L, Best M, Saddler JN. Screening of microorganisms with the potential for biological control of sapstain in unseasoned lumber. Mater Org (Berl) 1988;23:81-95.
16. Oelschlaeger TA, Tall BD. Invasion of cultured human epithelial cells by Klebsiella pneumoniae Isolated from the Urinary Tract. Infection Immunity Am Soc Microbiol 1997;65:2950–8.
17. Abdullah M, Al-Sadi, Al-Alawi ZA, Patzelt A. Association of Alternaria alternata and Cladosporium cladosporioides with leaf spot in Cissus quadrangularis and Ficus sycomorus. Plant Pathol J 2015;14:44-4.
18. Strobel GA, Tomsheck A, Geary B, Spakowicz D, Strobel SA, Mattner S, et al. Endophyte strain NRRL 50072 producing volatile organics is a species of ascocoryne. Mycology 2010;1:187–94.
19. Babu PR, Sarma VV. Fungi as promising biofuel resource. New and Future Developments in Microbial Biotechnology and Bioengineering; 2019.
20. Duran N, Marcato PD, Duran M, Yadav A, Gade A, Rai M. Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi and plants. Appl Microbiol Biotechnol 2011;90:1609–24.
21. Gade AK, Bonde P, Ingle AP, Marcato PD, Duran N, Rai MK. Exploitation of Aspergillus niger for synthesis of silvernanoparticles. Biobased Mater Bioenergy 2008;2:243–7.
22. Velusamy P, Kumar GV, Jeyanthi V, Das J, Pachaiappan R. Bio-inspired green nanoparticles: synthesis, mechanism, and antibacterial application. Toxicol Res 2016;32:95–102.
23. Vahabi K, Mansoori GA, Karimi S. Biosynthesis of silver nanoparticles by fungus Trichoderma reesei. Insci J 2011;1:65–79.
24. Alghuthaymi MA, Almoammar H, Rai M, Said Galiev E, Abd Elsalam KA. Myconanoparticles: synthesis and their role in phytopathogens management. Biotechnol Biotechnol Equip 2015;29:221–36.
25. Costa Silva LP, Oliveira JP, Keijok WJ, Silva AR, Aguiar AR, Guimarães MCC, et al. Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagus fungus duddingtonia flagans. Int J Nanomed 2017;12:6373–81.
26. Guilger M, Pasquoto Stigliani T, Bilesky Jose N, Grillo R, Abhilash PC, Fraceto LF, et al. Biogenic silver nanoparticles based on Trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity. Sci Rep 2017;7:44421.
27. Mekkawy AI, El-Mokhtar MA, Nafady NA, Yousef N, Hamad MA, El-Shanawany SM, et al. In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels. Int J Nanomed 2017;12:759–77.
28. Ottoni CA, Simões MF, Fernandes S, Santos JG, Silva ES, Souza RFB, et al. Screening of filamentous fungi for antimicrobial silver nanoparticles synthesis. AMB Express 2017;7:31.
29. Sanghi R, Verma P. Biomimetic synthesis and characterization of protein capped silver nanoparticles. Bioresour Technol 2009;100:501–4.
30. Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir 1996;12:788.
31. Henglein A. Physicochemical properties of small metal particles in solution: microelectrode reactions, chemisorption, composite metal particles, and the atom-to-metal transition. J Phys Chem 1993;97:5470-1 .
32. Ahmad A, Mukherjee P, Mandal D, Senapati S, Khan MI, Kumar R, et al. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B Biointerfaces 2003;28:313.
33. Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Curr Nanosci 2008;4:141–4.
34. Senapati S. Biosynthesis and Immobilization of nanoparticles and their applications. Ph. D. thesis, University of Pune; 2005.
35. Senapati S, Ahmad A, Khan MI, Sastry M, Kumar R. Extracellular biosynthesis of bimetallic au-ag alloy nanoparticles. Small 2005;1:517-20.
36. Valodkar M, Jadeja RN, Thounaojam MC, Devkar RV, Thakore S. Biocompatible synthesis of peptide capped copper nanoparticles and their biological effect on tumor cells. Materials Chem Physics 2011;128:83–9.
37. William DH, Fleming I. Spectroscopic methods in organic chemistry. 3rd ed. London: McGraw-Hill Book Co. (UK), Ltd; 1980. p. 1–73.
38. Guangquaum Li, Dan H, Yongqing Q, Wang LI. Fungus mediated green synthesis of silver nanoparticles using Aspergillus terreus. Int J Mol Sci 2012;13:466-76.
39. Khalil MM, Ismail EM, El-Baghdady KZ, Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem 2013;7:1131-9.
40. Li WR, Xie XB, Shi QS, Duan SS, Yang O, Chen YS. Antibacterial effect of silver nanoparticles on Staphylococcus aureus. Biometals 2011;24:135-41.
41. Keshavamurthy M, Srinath BS, Rai VR. Phytochemicals mediated green synthesis of gold nanoparticles using Pterocarpus santalinus L. (Red Sanders) bark extract and their antimicrobial properties. Part Sci Technol Int J 2017;36:7.
42. Ahluwalia V, Kumar J, Sisodia R, Shakil NA, Walia S. Green synthesis of silver nanoparticles by Trichoderma harzianum and their bioefficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia. Ind Crops Prod 2014;55:202–6.
43. Balakumaran MD, Ramachandran R, Kalaicheilvan PT. Exploitation of endophytic fungus, Guignardia mangiferae for extracellular synthesis of silver nanoparticles and their in vitro biological activities. Microbiol Res 2015;178:9–17.
44. Elgorban AM, Aref SM, Seham SM, Elhindi KM, Bahkali AH, Sayed SR, et al. Extracellular synthesis of silver nanoparticles using Aspergillus versicolor and evaluation of their activity on plant pathogenic fungi. Mycosphere 2016;7:844–52.
45. Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine: NBM 2009;5:382–6.
46. Gopinath V, Velusamy P. Extracellular biosynthesis of silver nanoparticles using Bacillus sp. GP-23 and evaluation of their antifungal activity towards Fusarium oxysporum. Spectrochim Acta Part A 2013;106:170–4.
47. Krishnaraj C, Ramachandran R, Mohan K, Kalaichelvan PT. Optimization for rapid synthesis of silver nanoparticles and its effect on phytopathogenic fungi. Spectrochim Acta Part A 2012;93:95–9.
48. Pinto RJB, Almeida A, Fernandes SCM, Freire CSR, Silvestre AJD, Neto CP. Antifungal activity of transparent nanocomposite thin films of pullulan and silver against Aspergillus niger. Colloids Surf B 2013;103:143–8.
49. Thenmozhi M, Kannabiran K, Kumar R, Khanna VG. Antifungal activity of Streptomyces sp. VITSTK7 and its synthesized Ag2O/Ag nanoparticles against medically important Aspergillus pathogens. J Mycologie Med 2013;23:97–103.
50. Prabhu S, Poulose EK. Silver Nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett 2012;2:32.
51. Rai M, Kon K, Ingle A, Duran N, Galdiero S, Galdiero M. Broad-spectrum bioactivities of silver nanoparticles: the emerging trends and future prospects. Appl Microbiol Biotechnol 2014;98:1951–61.
52. Gupta RK, Kumar V, Gundampati RK, Malviya M, Hasan SH, Jagannadham MV. et al. Biosynthesis of silver nanoparticles from the novel strain of Streptomyces Sp. BHUMBU-80 with highly efficient electroanalytical detection of hydrogen peroxide and antibacterial activity. J Environ Chem Eng 2017;5:5624–35.
53. Loo YY, Rukayadil Y, Nor-Khaizura MAR, Kuan CH, Chieng BW, Nishibuchi M, et al., In vitro antimicrobial activity of green synthesized silver nanoparticles against selected gram-negative foodborne pathogens. Front Microbiol 2018;9:1555.
Statistics
17 Views | 24 Downloads
Citatons
How to Cite
NAIR, B., D. ABRAHAM, A. DINESH, and G. E. M. SWAMY. “ ‘MYCO SYNTHESIS OF SILVER NANOPARTICLES USING GLIOCLADIUM ROSEUM (CLONOSTACHYS ROSEA (LINK) SCHROERS, SAMUELS) AND ITS ANTIMICROBIAL EFFICACY AGAINST SELECTED PATHOGENS’”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 6, Nov. 2020, pp. 77-84, doi:10.22159/ijcpr.2020v12i6.40293.
Section
Original Article(s)