ISOLATION OF PHYTOCONSTITUENT, IN VITRO ANTICANCER STUDY IN HELA AND MCF-7 CELL LINES AND MOLECULAR DOCKING STUDIES OF POTHOS SCANDENS LINN
Keywords:Pothos scandens, Phytoconstituent, Anticancer, MTT assay, Molecular docking
Objective: This study aims to isolate an active phytoconstituent from ethanolic leaf extract of Pothos scandens Linn., to evaluate in vitro anticancer activity, and to carry out molecular docking studies of the isolated phytoconstituent.
Methods: The bioactive constituent 1,1’-(4,5-dihydroxy benzene-1,2-diyl) bisoct-7-en-1-one, a phenolic compound, was isolated by using chromatographic methods and the structure was elucidated by various spectroscopic techniques. In vitro anticancer activity was evaluated against HeLa and MCF 7 cell lines. The viability of cells was evaluated by direct observation of cells by an Inverted phase-contrast microscope and by the MTT assay method. IC50 was calculated using the linear regression model.
Results: The results of anticancer studies revealed that different concentrations of the ethanolic extract of leaves of Pothos scandens Linn. exhibited cytotoxic activity against HeLa and MCF 7 cell lines with IC50 of 22.9 and 18.32 μg/ml, respectively. The anticancer potential of the plant was revalidated by in silico molecular docking study with Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, PDB ID: 4AG8) using Discovery studio 2018. Results of the docking study showed that the ligand exhibited strong interaction with the VEGFR2 kinase with significant binding energy.
Conclusion: Pothos scandens linn. can be used for the isolation of potent anticancer agents.
RS Sathoskar, SD Bhandarkar, SS Ainapure. Pharmacology and pharmacotherapeutics. 17th ed. Mumbai: Popular Prakashan; 2001. p. 800-1.
Alfarouk KO, Stock CM, Taylor S, Walsh M, Muddathir AK, Verduzco D. Resistance to cancer chemotherapy-failure in drug response from ADME to P-gp. Cancer Cell Int 2015;15:71.
Javed Iqbal, Banzeer Ahsan Abbasi, Tariq Mahmood, Sobia Kanwal, Barkat Ali, Sayed Afzal Shah, Ali Talha Khalil. Plant-derived anticancer agents-a green anticancer approach. Asian Pac J Trop Biomed 2017;7:1129-50.
Abdul Wahab Siti Mariam, Jantan Ibrahim, Haque Md Areeful, Arshad Laiba. Exploring the leaves of annona muricata L. as a source of potential anti-inflammatory and anticancer agents. Front Pharmacol 2018;9:661.
Thakore P, Mani RK, Kavitha SJ. A brief review of plants having anti-cancer property. Int J Pharm Res Dev 2012;3:129-36.
Tariq A, Sadia S, Pan K, Ullah S, Mussarat F. A systematic review on ethnomedicines of anti-cancer plants. Phytother Res 2017;31:202-64.
Li-Rui Sun, Wei Zhou, Hong-Mei Zhang, Qiu-Shi Guo, Wei Yang, Bing-Jin Li, et al. Modulation of multiple signaling pathways of the plant-derived natural products in cancer. Front Oncol 2019;9:1153.
Mansor M, Boyce PC, Othman AS, Sulaiman B. The araceae of peninsular Malaysia, Penerbit USM; 2012. p. 1-146.
DeFilipps RA, Krupnick GA. The medicinal plants of Myanmar. Phytokeys 2018;102:1–341.
Das AK, Dutta BK, Sharma GD. Medicinal plants used by different tribes of Cachar district, Assam. IJTK 2008;7:446-54.
Niral H, Sojeetra, Rabinarayan Acharya A. Review on ethnomedicinal claims and spread of Pothos scandens L. Eur J Med Plants 2020;31:22–8.
Santhosh Kumar JU, Krishna Chaitanya MJ, Andrew J Semotiuk, Krishna V. Indigenous knowledge on medicinal plants used by ethnic communities of South India. Ethnobot Res Appl 2019;18:1-112.
Savinaya MS, Patil SS, Narayana J, Krishna V. Traditional medicine knowledge and diversity of medicinal plants in sharavathi valley region of central-western ghats. Int J Herb Med 2016;4:124-30.
Ayyanar M, Ignacimuthu S. Traditional knowledge of kani tribals in kouthalai of tirunelveli hills, Tamil Nadu, India. J Ethnopharmacol 2005;102:246-55.
Inta A, Shengji P, Balslev H, Wangpakapattanawong P, Trisonthi C. A comparative study on medicinal plants used in Akha's traditional medicine in China and Thailand, cultural coherence or ecological divergence. J Ethnopharmacol 2008;116;508-17.
Kamal HM, Bidur S, Kaium SMA, Mazumder K. Anti-diabetic and bronchodilator activities of Pothos scandens linn leaves. Int J Pharm Phytochem Res 2015;7:1202-7.
Vinayaka KS, Prashith Kekuda TR, Rajkumar N, Chandrasekhar MB. Studies on antibacterial, anthelmintic and larvicidal efficacy of Pothos scandens L. Res J Pharm Technol 2009;2:850-3.
Thankarajan Sajeesh, Karuppusamy Arunachalam, Tangaraj Parimelazhagan. Antioxidant and antipyretic studies on pothos scandens L. Asian Pac J Trop Med 2011;4:889-99.
Harborne JB. Phytochemical methods–a guide to modern techniques of plant analysis. 3rd ed. London: Chapman and Hall; 1984. p. 1-7.
Kumar R, Murugananthan G, Nandakumar K, Talwar S. Isolation of anxiolytic principle from ethanolic root extract of Cardiospermum halicacabum. Phytomedicine 2011;18:219–23.
Shanmugam Prakash, Nagaraj Elavarasan, Kasivisvanathan Subashini, Selvaraj Kanaga, Ramamurthy Dhandapani, Magudeeswaran Sivanandam, et al. Isolation of hesperetin-a flavonoid from Cordia sebestena flower extract through antioxidant assay guided method and its antibacterial, anticancer effect on cervical cancer via in vitro and in silico molecular docking studies. J Mol Struct 2020;1207:1-13.
Wan Mohd Zain WN, Rahmat A, Othman F, Yap TY. Antiproliferative properties of clausine-b against cancer cell lines. Malays J Med Sci 2009;16:29-34.
Mosmann T. Rapid colorimetric assay for cellular growth and survival-application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Ferrari M, Fornasiero MC, Isetta AM. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 1990;131:165-72.
Himali Somaweera, Gary C, Lai, Rachel Blackeye, Beverly Littlejohn, Justine Kirksey, et al. Ethanolic extracts of california mugwort (Artemisia douglasiana Besser) are cytotoxic against normal and cancerous human cells. J Herb Med 2013;3:47–51.
Asadollahi M, Firuzi O, Heidary Jamebozorgi F, Alizadeh M, Jassbi AR. Ethnopharmacological studies, chemical composition, antibacterial and cytotoxic activities of essential oils of eleven salvia in Iran. J Herb Med 2018;20:100-250.
Barile FA. Continuous cell lines as a model for drug toxicity assessment. In: Jose Castell, Maria Gmez-Lechn. (Eds.) In vitro methods in pharmaceutical research. E-Publishing Inc. London: Academic Press; 1997. p. 33–54.
AL-Zuaidy MH, Ismail A, Mohamed S, Razis AFA, Mumtaz MW, Hamid AA. Antioxidant effect, glucose uptake activity in cell lines and cytotoxic potential of Melicope Lunu-Ankenda leaf extract. J Herb Med 2018;14:55-60.
McTigue M, Murray BW, Chen JH, Deng YL, Solowiej J, Kania RS. Molecular conformations, interactions, and properties associated with drug efficiency and clinical performance among VEGFR TK inhibitors. PNAS 2012;109:18281–9.
Roy K, Kar S, Das RN. Understanding the basics of QSAR for applications in pharmaceutical sciences and risk assessment. Ist ed. London: Academic Press; 2015. p. 357–425.
Kaloni D, Chakraborty D, Tiwari A, Biswas S. In silico studies on the phytochemical components of Murraya koenigii targeting TNF-α in rheumatoid arthritis. J Herb Med 2020;24:100396.
Suffnes M, Pezzuto JM. Assays related to cancer drug discovery. In: Hostettmann K. (Ed.) Methods in Plant Biochemistry: Assays for Bioactivity. Vol. 6. London: Academic Press; 1990. p. 71–133.
Itharat A, Houghton PJ, Eno-Amooquaye E, Burke PJ, Sampson JH, Raman A. In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J Ethnopharmacol 2004;90:33-8.
How to Cite
Copyright (c) 2021 SEEMA S. NAIR, JOYAMMA VARKEY
This work is licensed under a Creative Commons Attribution 4.0 International License.