• SURAJ KUMAR SHAH Department of Pharmaceutical Technology, School of Medical Science, Adamas University, Kolkata 700126, West Bengal, India
  • SANJAY DEY Department of Pharmaceutical Technology, School of Medical Science, Adamas University, Kolkata 700126, West Bengal, India
  • SOURAV DE Department of Pharmaceutical Technology, School of Medical Science, Adamas University, Kolkata 700126, West Bengal, India



Atorvastatin, Atenolol, RP-HPLC, Pharmacokinetic study, Rabbit, Bilayer tablet


Objective: The objective of the present study is to establish a simple, sensitive, and specific RP-HPLC technique to determine atorvastatin calcium (ATV) and atenolol (ATL) in rabbit plasma.

Methods: Both the drugs like atorvastatin calcium and atenolol have been extracted by simple liquid-liquid extraction with acetonitrile.

Results: The chromatographic separation has performed on a reversed-phase C18 column with a mobile phase of phosphate buffer (pH 4.0): acetonitrile: methanol (40:40:20, v/v). The techniques have been validated over the concentration range of 22.08-4778.37 ng ml-1 for ATV and 18.35-3969.20 ng ml-1 for ATL in rabbit plasma. The acceptability range was satisfied for all validation criteria. These methods have been profitably applied to the pharmacokinetic study of a bilayer tablet of ATV and ATL after an oral administration to a rabbit. A significant recovery of the drug from plasma resulted from acetonitrile as extracting solvent compared to other organic solvents.

Conclusion: The results obtained proved that the RP-HPLC method developed was accurate and reproducible, and the drug was stable in rabbit plasma.


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How to Cite

SHAH, S. K., S. DEY, and S. DE. “SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND ATENOLOL IN RABBIT PLASMA BY RP-HPLC METHOD AND ITS APPLICATION IN PHARMACOKINETIC STUDY”. International Journal of Current Pharmaceutical Research, vol. 14, no. 2, Mar. 2022, pp. 72-78, doi:10.22159/ijcpr.2022v14i2.1968.



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