DETERMINATION OF S-METHYL L-CYSTEINE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD
Keywords:Amino acid determination, Analytical method validation, High performance liquid chromatography, S-Methyl L-Cysteine, 3-methylthioalanine
Objective: A simple, reproducible and sensitive high-performance liquid chromatography method has been developed for determination of S-Methyl L-Cysteine. S-Methyl L-Cysteine is widely observed and most common amino acid in plants, including many edible vegetables, which is responsible for reducing blood cholesterol level in the body.
Methods: S-Methyl L-Cysteine was chromatographed using Phosphate buffer of pH 6.5: Acetonitrile in the ratio of 97:3. The liquid chromatogram was equipped with a variable wavelength UV detector, an injector and a data processor. Inertsustain GL-Science Column C-18 (150 mm x 4.6 mm; 5μ) was used as a stationary phase.
Results: The retention time of S-Methyl L-Cysteine was observed as 2.261±0.0016 min. The linearity value for S-Methyl L-Cysteine was found to be 100-2000 µg/ml with Correlation of Determination (R2) value as 0.9992. LOD and LOQ values obtained are 29.51μg/ml and 89.74 μg/ml, respectively.
Conclusion: The method was developed and validated successfully as per ICH guidelines for analytical method validation.
Maw GA. Biochemistry of S-methyl-L-cysteine and its principal derivatives. Sulfür Rep. 1982;2(1):1-26. doi: 10.1080/01961778208082422.
Doleman JF, Grisar K, Van Liedekerke L, Saha S, Roe M, Tapp HS. The contribution of alliaceous and cruciferous vegetables to dietary sulphur intake. Food Chem. 2017;234:38-45. doi: 10.1016/j.foodchem.2017.04.098, PMID 28551250.
Ingenbleek Y, Kimura H. Nutritional essentiality of sulfur in health and disease. Nutr Rev. 2013;71(7):413-32. doi: 10.1111/nure.12050, PMID 23815141.
Nicastro HL, Ross SA, Milner JA. Garlic and onions: their cancer prevention properties. Cancer Prev Res (Phila). 2015;8(3):181-9. doi: 10.1158/1940-6207.CAPR-14-0172, PMID 25586902.
Herr I, Büchler MW. Dietary constituents of broccoli and other cruciferous vegetables: implications for prevention and therapy of cancer. Cancer Treat Rev. 2010;36(5):377-83. doi: 10.1016/j.ctrv.2010.01.002, PMID 20172656.
Sivapalan T, Melchini A, Saha S, Needs PW, Traka MH, Tapp H. Bioavailability of glucoraphanin and sulforaphane from high-glucoraphanin broccoli. Mol Nutr Food Res. 2018;62(18):e1700911. doi: 10.1002/mnfr.201700911, PMID 29266773.
Sivapalan T, Melchini A, Coode Bate J, Needs PW, Mithen RF, Saha S. An LC-MS/MS method to measure S-methyl-l-cysteine and S-methyl-l-cysteine sulfoxide in human specimens using isotope labelled internal standards. Molecules. 2019;24(13):2427-36. doi: 10.3390/molecules24132427, PMID 31269651.
Komatsu W, Miura Y, Yagasaki K. Suppression of hypercholesterolemia in hepatoma-bearing rats by cabbage extract and its component, S-methyl-l-cysteine sulfoxide. Lipids. 1998;33(5):499-503. doi: 10.1007/s11745-998-0233-7, PMID 9625597.
Kyung KH, Fleming HP. Antimicrobial activity of sulfur compounds derived from cabbage. J Food Prot. 1997;60(1):67-71. doi: 10.4315/0362-028x-60.1.67, PMID 10465044.
Yoshinari O, Shiojima Y, Igarashi K. Anti-obesity effects of onion extract in Zucker diabetic fatty rats. Nutrients. 2012;4(10):1518-26. doi: 10.3390/nu4101518, PMID 23201769.
Edmands WMB, Gooderham NJ, Holmes E, Mitchell SC. S-methyl-l-cysteine sulphoxide: the Cinderella phytochemical? Toxicol Res. 2013;2(1):11-22. doi: 10.1039/C2TX20030A.
Keusgen M, Schulz H, Glodek J, Krest I, Krüger H, Herchert N. Characterization of SomeAlliumHybrids by aroma precursors, aroma profiles, and alliinase activity. J Agric Food Chem. 2002;50(10):2884-90. doi: 10.1021/jf011331d, PMID 11982415.
Krest I, Glodek J, Keusgen M. Cysteine sulfoxides and alliinase activity of SomeAlliumSpecies. J Agric Food Chem. 2000;48(8):3753-60. doi: 10.1021/jf990521+, PMID 10956182.
Montano A, Beato VM, Mansilla F, Orgaz F. Effect of genetic characteristics and environmental factors on organosulfur compounds in garlic (Allium sativum L.) grown in Andalusia, Spain. J Agric Food Chem. 2011;59(4):1301-7. doi: 10.1021/jf104494j, PMID 21247176.
Davea HN, Makwanab AG, Suhagia BN. Validated reversed-phase high-performance liquid chromatographic method for determination of three novel steroids in bulk and pressurized metered–dose commercial preparations using a common mobile phase. Int J Appl Sci Eng. 2013;11(2):125-35.
Murnane D, Martin GP, Marriott C. Validation of a reverse-phase high-performance liquid chromatographic method for concurrent assay of a weak base (salmeterol xinafoate) and a pharmacologically active steroid (fluticasone propionate). J Pharm Biomed Anal. 2006;40(5):1149-54. doi: 10.1016/j.jpba.2005.09.028, PMID 16280225.
International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceutical for Human Use, Validation of Analytical Procedures: Text and Methodology Q2. Vol. R1; 2005. p. 1-13.
How to Cite
Copyright (c) 2022 BHAVESH PATEL, VIRAL SHAH, HARDIK SHAH, NEHAL PATEL, NAIMISHA PATEL
This work is licensed under a Creative Commons Attribution 4.0 International License.