EXTRACTION, IDENTIFICATION AND ANTIOXIDANT POTENTIAL OF SECONDARY METABOLITES OF WHOLE FRUITS OF SANTALUM ALBUM LINNBY GC-MS
Objective: In order to study the antioxidant potential responsible secondary metabolites the fruit pulp and kernels of Santalum albumwere extracted in different solvents and tried to identify by GC-MS.
Methods: The extraction was carried out in various organic solvents by soxhlet by successive manner. The antioxidant capacity of all extracts was determined by FRAP. Identification of various secondary metabolites in the extracts was done by analysing them by GC-MS.
Results: Ethyl acetate and methanol extracts of fruit pulp and kernel have shown antioxidant activity. GC-MS identification has shown the presence of many secondary metabolites. Among many compounds cholest-4-en-3-one [11.86 %] obtained as the major constituent in fruit pulp di-chloromethane extract. While kernel methanol extract have showed presence of pyrazinamide [63.70%] and Acetamide-2-cyano [55.40%] in acetonitrile as major compounds.
Conclusion: The present study suggests that fruit pulp and kernels of Santalum album can be a new and rich source of medicinally useful secondary metabolites.
2. Navindra P Seeram. David heber impact of berry phytochemicals on human health: Effects beyond Antioxidation. ACS Symposium Series 2007;956:326â€“36.
3. PSC Sri Harsha, Mohammad Imtiyaj Khan, Puttachandra Prabhakar, Parvatam Giridhar. Cyanidin-3-glucoside, nutritionally important constituents and in vitro antioxidant activities of Santalum album L. Berries Food Res Int 2013;50:275â€“81.
4. Biswapriya B Misra, Satyahari Dey. Accumulation patterns of Phenylpropanoids and enzymes in East Indian sandalwood tree undergoing developmental progression in vitro. Astralian J Crop Sci 2013;7(5):681-90.
5. Kurt Aitzetmuller. Santalbic acid in the plant kingdom. Plant Syst Evol 2012;12:678-5.
6. Ashok K, Jayprakash P. Screening of active phytocompounds by GCâ€“MS study and antimicrobial activity in the stem of santalum album. Int J Curr Pharm Res 2012;4:3.
7. Adeolu A Asedapo, Florence O Jimoh, Anthony J Afolayan, Patrick j Masika. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanttera oppositifolia and Adenia gummifera. BMC Complementary Altern Med 2008;8:54.
8. Sutha S, Kalpana Devi V, Mohan VR. GCMS Determination of Bioactive Components of Alstonia venenata R. Br Res J Pharm Biol Chem Sci 2012;3(2):291.
9. Xiangying Yua, b Mingyue Zhaob, FeiLiub Shitong Zengb, Jun Hu. Identification of 2, 3-dihydro-3, 5-dihydroxy-6-methyl-4H-pyran-4-one as a strong antioxidant in Glucose Histidine Maillard reaction products. Food Res Int 2013;51(1):397-403.
10. Hans Lingnert, George R Waller. Stability of antioxidants formedfrom histidine and glucose by the millard reaction. J Agric Food Chem 1983;31:27-30.
11. Wei Qing, Wang Xiao-jie, Chen Liang, Qiu Bo-han, Peng Chong. GC-MS analysis of the volatile oil from oldham gypsophila by simultaneous distillation extraction. Med Plant 2012;3(3):34-6.
12. Jin Zhen-guo, Liu Ping, Wang Xiang-ting. Analysis on chemical components of volatile oil in wisteria pods with GC/MS. J Shangluo University 2012;4:3-5.
13. Lin AS1, Engel S, Smith BA, Fairchild CR, Aalbersberg W, Hay ME, et al. Structure and biological evaluation of novel cytotoxic sterol glycosides from the marine red alga Peyssonnelia sp. Bioorg Med Chem 2008;18(23):8264-9.
14. Rishikesh Mankidy, Steve Wiseman, Hong Ma John, P Giesy. Biological Impact of phthalates. Toxicol Lett 2013;217:50â€“8.
15. Takahashi C1, Kikuchi N, Katou N, Miki T, Yanagida F, Umeda M. Possible anti-tumour-promoting activity of components in Japanese soybean fermented food, Natto: Effect on gap junctional intercellular communication. Carcinogenesis 1995;16(3):471-6.
16. Kim SJ, Chung WS, Kim SS, Ko SG, Um JY. Antiinflammatory effect of Oldenlandia diffusa and its constituent, hentriacontane, through suppression of caspase-1 activation in mouse peritoneal macrophages. Phytother Res. 2011;25(10):1537-46.
17. Atolani Olubunmi, Olatunji A Gabriel. Epicuticular wax and volatiles of kigelia pinnata leaf extract. Ethnobotanical Leaflets 2010;14:797-806.
18. Ahmed Ali Fadda, Samir Bondock, Ramy R Abie, Hassan Ali Etman. Cyano-acetamide derivatives as synthons in heterocyclic synthesis. Turk J Chem 2008;32:259â€“86.
19. Bryn H Daisy, Gary A Strobel, Uvidelio Castillo, David Ezra, Joe Sears, David K Weaver, et al. Naphthalene an insect repellent, is produced by Muscodor vitigenus, a novel endopathic fungus. Microbil 2002;148:3737-41.
20. Y Zhang, D Mitchison. The curious characteristics of pyrazinamide: a review. Int J Tuberc Lung Dis 2003;7(1):6â€“21.