STUDY OF CRUDE EXTRACTS FROM CASSIA SIEBERIANA ROOT BARK AND KHAYA GRANDIFOLIOLA TRUNK BARK: PHYTOCHEMICAL SCREENING, QUANTITATIVE ANALYSIS AND RADICAL SCAVENGING ACTIVITY


Lancine Traore, Yves-alain Bekro, Jean-luc Pirat, Janat Akhanovna Mamyrbeva-bekro

Abstract


Objective: Cassia sieberiana and Khaya grandifoliola are two plants commonly used in traditional medicine in Côte d’Ivoire. Photochemical screening of crud extract obtained from C. sieberiana root bark and K. grandifoliola trunk bark revealed the presence of alkaloids, sterols, terpenes, polyphenols, flavonoids, coumarins, tannins, reducing sugars, glycosides, carbohydrates, cardiac glycosides and saponins.

Methods: Quantitative analysis was screened in C. sieberiana root bark and K. grandifoliola trunk bark.

Results: The results respectively showed high concentrations of total phenols (225.57±7.57 and 186.75±12.76 μgGAE/mg), total flavonoids (64.70±5.25 and 117.88±8, 68 μgQE/mg) and total tannins (170.60±5.85 and 39.96±1, 58 μgTAE/mg). The antioxidant activity of the glycosides extracts CS1, KG1 and their corresponding aglycones CS2, KG2 of these plants has been studied by scavenging free radicals by DPPH and that, compared with L-ascorbic acid (vitamin C, IC50 = 0.07 µg/ml). IC50 values of CS1 (2.69 µg/ml), KG1 (3.16 µg/ml) and CS2 (1.30 µg/ml), KG2 (0.726 µg/ml) showed that the aglycones are clearly more effective than the glycosides.

Conclusion: Qualitative analysis of Cassia sieberiana root bark and Khaya grandifoliola trunk bark showed a presence of a variety of secondary metabolites in these plants, when the quantitative analysis concludes that they contain phenolic compounds, flavonoids and tannins to varied contents.

Keywords: Cassia sieberiana, Khaya grandifoliola, Glycoside, Aglycone, Phytochemical screening, Quantitative analysis, DPPH, IC50, Côte d’Ivoire.

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Title

STUDY OF CRUDE EXTRACTS FROM CASSIA SIEBERIANA ROOT BARK AND KHAYA GRANDIFOLIOLA TRUNK BARK: PHYTOCHEMICAL SCREENING, QUANTITATIVE ANALYSIS AND RADICAL SCAVENGING ACTIVITY

Date

15-07-2015

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Journal

International Journal of Current Pharmaceutical Research
Vol 7, Issue 3, 2015 Page: 22-26

Online ISSN

0975-7066

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Authors & Affiliations

Lancine Traore
Laboratoire de Chimie Bio Organique et de Substances Naturelles (LCBOSN), Unité de Formation et de Recherche des Sciences Fondamentales et Appliquées (UFR SFA), Université Nangui Abrogua, 02 BP 0801 Abidjan 02, Côte d’Ivoire, Laboratoire Architecture Moléculaire et Matériaux Nanostructurés, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), 8 Rue de L’Ecole Normale F-34296 Montpellier Cedex 5, France

Yves-alain Bekro
Laboratoire de Chimie Bio Organique et de Substances Naturelles (LCBOSN), Unité de Formation et de Recherche des Sciences Fondamentales et Appliquées (UFR SFA), Université Nangui Abrogua, 02 BP 0801 Abidjan 02, Côte d’Ivoire

Jean-luc Pirat
Laboratoire Architecture Moléculaire et Matériaux Nanostructurés, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), 8 Rue de L’Ecole Normale F-34296 Montpellier Cedex 5, France

Janat Akhanovna Mamyrbeva-bekro
Laboratoire de Chimie Bio Organique et de Substances Naturelles (LCBOSN), Unité de Formation et de Recherche des Sciences Fondamentales et Appliquées (UFR SFA), Université Nangui Abrogua, 02 BP 0801 Abidjan 02, Côte d’Ivoire


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