Int J Curr Pharm Res, Vol 7, Issue 3, 58-59Original Article



Department of Advanced Zoology and Biotechnology, Presidency College, Chennai 600005

Received: 02 May 2015, Revised and Accepted: 05 Jun 2015


Objective: In the present study the antioxidant potential and anti-inflammatory effect of qucertin compound was assessed.

Methods: The antioxidant potential of the drug was determined by ORAC assay and DPPH assay. Likewise for anti-inflammatory studies, RBC’s were collected from healthy volunteers and the hemo protective activity of the drug was carried out at various concentrations.

Results: The results showed that qucertin has a positive effect on both the parameters. The probable reasons governing the facts are discussed in the light of previous literature.

Conclusion: The results show that qucertin seems to be a good replacement for chemical therapeutic drugs as it has antioxidative and anti-inflammatory properties.

Keywords: Qucertin, Antioxidant, Anti-inflammatory, Hemoprotective.


Flavonoids, a large group of natural polyphenolic compounds, are powerful antioxidants found in various fruits, vegetables, tea, red wine, and medicinal herbs. Flavonoids can scavenge free radicals and other oxidizing intermediates because of their phenolic hydroxyl groups and thus contribute to the counteraction of body against a great variety of diseases [1].

Quercetin is a unique bioflavonoid that has been extensively studied by researchers over the past 30 years. Bioflavonoids were first discovered by Nobel Prize laureate Albert Szent Gyorgyi in the year 1930. Flavonoids belong to a group of natural substances with variable phenolic structure and are found in the fruits, vegetables, grains, bark roots, stem, flowers, tea and wine [2]. These natural products were known for their beneficial effects on health long before flavonoids were isolated as the effective compounds. More than 4000 varieties of flavonoids have been identified, many of which are responsible for their attractive colors of flowers, fruits and leaves [3].

In view of this, the present study has been taken to determine the antioxidant potential and anti-inflammatory effects of questin, to elucidate it as a potential drug for various ailments.


Qucertin compound was purchased from Sigma Aldrich, USA and used for the present study.

In vitro antioxidant activity

ORAC assay

The antioxidant potential of qucertin was determined by ORAC assay following the methods of Huang et al. (2002, 2005) [4, 5].

In vitro Anti-inflammatory activity

Membrane stabilization assay

Qucertin was subjected to human red blood cell (HRBC) membrane stabilization method to study the anti-inflammatory activity according to the method of Gandhidasan et al. (1991) [6].


In vitro antioxidant activity

Table-1 presents the data on antioxidant potential of qucertin when tested with ORAC assay. The data reveals that rutin has antioxidant potential, as the value of Net Relative Fluorescence Unit (NRFU) increased with an increase in the concentration of the drug. The NRFU values are Maximum at 100 µM (75.77+0.047) and decreases as the concentration is increased further.

Similarly when DPPH assay of qucertin was carried out, among all the concentrations of rutin, 12.5 µM showed the maximum % DPPH inhibition (Table-2).

The results indicate that qucertin has antioxidant potential and can be used as a drug for combating various ailments.

Table 1: ORAC Assay for Qucertin in different concentrations

S. No. Concentration of qucertin (µM) Net relative fluorescence unit
1 12.5 49.7+0.808
2 25 69.44+0.074
3 50 73.32+0.038
4 100 75.77+0.047
5 200 69.7+0.027

Values are mean+SE of six individual observations.

Table 2: DPPH Assay of Qucertin

S. No. Concentration of Qucertin (µM) % DPPH inhibition
1 12.5 0.294+0.003
2 25 0.148+0.047
3 50 0.079+0.041
4 100 0.077+0.160
5 200 0.070+0.050

Values are mean+SE of six individual observations.

Table 3: Haemoprotective activity of Qucertin

S. No. Concentration of Qucertin (µM) % Protection
1 62.5 97.348+0.370
2 125 62.219+0.907
3 250 97.028+0.140
4 500 96.896+0.252
5 1000 96.967+0.121

Values are mean+SE of six individual observations.

Haemoprotection of qucertin

When qucertin was tested for its hemoprotective activity, the per cent hemoprotection was directly proportional to concentration; the values being 97.348+0.370 at 62.5 µM and 96.967+0.121 at 1000 µM (table 3). The results thus reveal us that qucertin has hemoprotective effect.


According to Meena et al. (2008) [7], more than 2000 flavonoids have been reported among woody and non-woody plants [8]. Biosynthesis, isolation techniques and preparative chromatography [9], TLC, UV and IR spectral studies have provided new dimensions to the chemistry of flavonoids to such an extent that their presence have become important taxonomically [10]. Presence of flavonoids has been reported from many plant species like Lycium barbarum [11]; Passiflora plamer [12]; Cassia angustifolia [13]; Jatropa curcas L.[14].

Quercetin has been reported from many plant species like Cicer arietinum Linn. [15] and Acacia catechu [16]. As mentioned earlier, since quercetin has anti-inflammatory, antioxidant and anticancer properties,isolation and extraction of this compound in vivo (leaf, stem, fruit, root) and in vitro callus from Citrullus colocynthis and its enhancement by addition of elicitors in culture, can be exploited further for largescale production of this medicinally important compound [7].

The antioxidant activity of quercetin is well known as it possesses a suitable structure for free radical scavenging and ion chelation. However, the results of the TBARS assay indicate that unlike galangenin, both analogues do not show significant antioxidant activity. This may be due to the presence of an additional 5–OH group in galangenin generating a structure for effectively scavenging the free radical. Absence of 5–OH group in Q–Cl and Q–OCH3 leads to a drop in the antioxidant activity. Quercetin is also known to chelate iron, which is responsible for the production of free radicals. The chelation involves 3’, 4’-hydroxy groups thus highlighting the importance of the catechol-like moiety [17].


The results show that qucertin seems to be a good replacement for chemical therapeutic drugs as it has antioxidative and anti-inflammatory properties.


The authors are thankful to V Clin Bio Labs (P) Ltd, Sri Ramachandra University, Porur, Chennai for the technical support.


Declared None.


  1. Roeder E. Medicinal plants in Europe containing pyrrolizidine alkaloids. Pharmazie 1995;50:83–98.
  2. Middleton EJ. Effect of plant flavonoids on immune and inflammatory cell functions. Adv Exp Med Biol 1998;439:175-82.
  3. De Groot H, Rauen U. Tissue injury by reactive oxygen species and the protective effects of flavonoids. Fundam Clin Pharmacol 1998;12:249-55.
  4. Huang D, Ou B, Hampsch-Woodill M, Flanagan J, Prior R. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 2002;50:4437-44.
  5. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
  6. Gandhidasan R, Thamaraichelvan A, Baburaj S. Anti-inflammatory action of Lannae coromandelica by HRBC membrane stabilization. Fitoterapia 1999;62:81-3.
  7. Meena MC, Patini V. Isolation and identification of flavonoid “Qucertin” from Citrullus colocynthis (Linn.) Schrad. Asian J Exp Sci 2008;22(1):137-42.
  8. Harborne JB. Plant phenolics. In: secondary plant products (Eds) Bell EA, Charlewood BV, Springer Verlag Berlin; 1981. p. 320.
  9. Casteel HW, Wender SM. Identification of flavonoid compounds, Rf values and colour tests. Anal Chem 1953;25l:508.
  10. Smith EB. In: Prospective in phytochemistry (Eds) Harborne JB, Swain T. Academic Press; London: 1969.
  11. Harsh ML, Nag TN, Jain S. Arid zone plants of Rajasthan a source of antimicrobials. Comp Physiol Ecol 1983;8:129-31.
  12. Ulubelen A, Mabry JJ, Dellamonicas G, Chopin J. Flavonoids of Passiflora plamer. J Nat Prod 1984;47:384-5.
  13. Goswami A, Reddi A. Antimicrobial activity of flavonoids of medicinally important plant Cassia angustifolia in vivo and in vitro. J Phytol Res 2004;17:179-81.
  14. Saxena S, Sharma R, Rajore S, Batra A. Isolation and identification of flavonoid "Vitexin" from Jatropha curcas L. J Plant Sci Res 2005;21:116-7.
  15. Joshi RS. Biosynthesis of primary and secondary products from in vivo and in vitro tissue cultures of some medicinal plants. Ph. D. Thesis, University of Rajasthan, Jaipur, India; 1985.
  16. Jain R, Patni V, Arora DK. Isolation and identification of flavonoid "quercetin" from Acacia catechu (L. f.) Willd-A Katha yielding plant. J Phytol Res 2007;20:43-5.
  17. Ferrali C, Signorini B, Caciotti. Protection against oxidative damage of erythrocyte membrane by the flavonoid quercetin and its relation to iron chelating activity. FEBS Lett 1997;416:123–9.

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International Journal of Current Pharmaceutical Research
Vol 7, Issue 3, 2015 Page: 58-59

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Authors & Affiliations

Muthukala B
Department of Advanced Zoology and Biotechnology, Presidency College, Chennai 600005

Sivakumari K
Department of Advanced Zoology and Biotechnology, Presidency College, Chennai 600005

Ashok K
Department of Advanced Zoology and Biotechnology, Presidency College, Chennai 600005


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