OPTIMIZATION OF CULTURAL PARAMETERS FOR THE PRODUCTION OF ANTIMICROBIAL COMPOUND FROM ENTEROCOCCUS FAECIUM CST-1 (MCC-2729)


Chandra Mouli Lalam, T Srinivasan, Vssl Prasad Talluri, Naidu P Y

Abstract


Objective: To improve the productivity of antibacterial compounds of Eenterococcus feacium CST-1 by optimizing its nutrient and physical factors and screened for its antimicrobial activity by agar well diffusion method.

Methods: In order to improve its efficiency, the effects of medium components carbon and nitrogen sources, temperature, pH, agitation, incubation time, were optimized and its productivity was determined by agar well diffusion method against four bacterial strains obtained from MTCC, Chandigarh, India namely Bacillus subtilis, E. coli, Psudomonas aeruginosa, Staphylococcus aureus,.

Results: The bacterial inhibition rate was more in the optimized medium composition (g/100 ml), containing tryptone 1.5, dextrose 3.0 and incubation time for 76 hrs, temperature 35±2 °C and pH 6.5. Compared to basal medium the optimized medium shown about 1.2 fold increased in the zone of inhibition by Enterococcus feacium CST-1.

Conclusion: The results from this study confirmed that the antibacterial substances produced by Eenterococcus feacium CST-1 were found to be more effective after its optimization.

Keywords: Enterococcus feacium, Zone of inhibition, Optimization, Antimicrobial compounds.

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References


Centeno JA, Menendez S, Rodrigues ottero JL. “Main microbial flora present as natural starters in Cebreiro raw cow’s-milk cheese (Northwest Spain)”. Int J Food Microbiol 1996;33:307–13.

Fox PF, Wallace JM. “Formation of flavour compoundsin cheese”. Adv Appl Microbiol 1997;45:17–85.

Piard JC, Desmazeaud M. “Inhibiting factors produced by lactic acid bacteria: Bacteriocins and other antibacterial substances Lait; 1992. p. 525-41.

Stiles ME, Holzapfel WH. “Lactic acid bacteria of foods and their current taxonomy”. Int J Food Microbiol 1997;36:1–29.

Vilches Mendez C, Hardisson C, Salas JA. “Biosynthesis of oleandomycin by Streptomyces antibioticus: influence of nutritional conditions and development of resistance”. J Gen Microbiol 1990;136:1447–54.

Chandra Mouli L, Naidu PY, Srinivasan T. “Isolation and screening of lactobacillus bacteria for ability to produce antibiotics”. Int Lett Nat Sci 2015;5:68-76.

Vandana Bali, Manab Bera B, Parmjit Panesar S. “Utilization of agro-industrial by products for bacteriocin production using newly isolated enterococcus faecium BS13”. Int J Biol Veterinary Agric Food Eng 2014;8:315-28.

Murray PR, Baroon EJ, Pfaller MA, Tenover FC, Yolke RH. “Manual of clinical microbiology”. Am Soc Microbiol 1995;6:115-26.

Moshood AY, Tengku Haziyamin Abdul, Tengku Abdul Hamid. “Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3”. N J Pharm 2012;2:49-59.

Ouardy Khay, Mustapha Ouhsassi, Abdeltif El Harsal, Mohamed Idaomar, Jamal Abrini. “Optimization of bacteriocin-like production by enterococcus durans E204 isolated from camel milk of morocco”. Curr Res Microbiol Biotechnol 2013;1:155-9.

Wefky SH, Abou-Elela GM, El-Bestawy E. “Optimization of fermentation conditions for bioactive compounds production by marine bacterium Enterococcus faecium”. J Appl Sci Res 2009;5:1445-54.




About this article

Title

OPTIMIZATION OF CULTURAL PARAMETERS FOR THE PRODUCTION OF ANTIMICROBIAL COMPOUND FROM ENTEROCOCCUS FAECIUM CST-1 (MCC-2729)

Date

15-07-2015

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 7, Issue 3, 2015 Page: 78-80

Online ISSN

0975-7066

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105 Views | 21 Downloads

Authors & Affiliations

Chandra Mouli Lalam
Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam 530045, India, Analytical research and development, Hospira, Chennai, India

T Srinivasan
Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam 530045, India

Vssl Prasad Talluri
Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam 530045, India,

Naidu P Y
Analytical research and development, Hospira, Chennai, India


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