OPTIMIZATION OF CULTURAL PARAMETERS FOR THE PRODUCTION OF ANTIMICROBIAL COMPOUND FROM ENTEROCOCCUS FAECIUM CST-1 (MCC-2729)
Objective: To improve the productivity of antibacterial compounds of Eenterococcus feacium CST-1 by optimizing its nutrient and physical factors and screened for its antimicrobial activity by agar well diffusion method.
Methods: In order to improve its efficiency, the effects of medium components carbon and nitrogen sources, temperature, pH, agitation, incubation time, were optimized and its productivity was determined by agar well diffusion method against four bacterial strains obtained from MTCC, Chandigarh, India namely Bacillus subtilis, E. coli, Psudomonas aeruginosa, Staphylococcus aureus,.
Results: The bacterial inhibition rate was more in the optimized medium composition (g/100 ml), containing tryptone 1.5, dextrose 3.0 and incubation time for 76 hrs, temperature 35Â±2 Â°C and pH 6.5. Compared to basal medium the optimized medium shown about 1.2 fold increased in the zone of inhibition by Enterococcus feacium CST-1.
Conclusion: The results from this study confirmed that the antibacterial substances produced by Eenterococcus feacium CST-1 were found to be more effective after its optimization.
2. Fox PF, Wallace JM. Formation of flavour compoundsin cheeseâ€. Adv Appl Microbiol 1997;45:17â€“85.
3. Piard JC, Desmazeaud M. Inhibiting factors produced by lactic acid bacteria: Bacteriocins and other antibacterial substances Lait; 1992. p. 525-41.
4. Stiles ME, Holzapfel WH. Lactic acid bacteria of foods and their current taxonomyâ€. Int J Food Microbiol 1997;36:1â€“29.
5. Vilches Mendez C, Hardisson C, Salas JA. Biosynthesis of oleandomycin by Streptomyces antibioticus: influence of nutritional conditions and development of resistanceâ€. J Gen Microbiol 1990;136:1447â€“54.
6. Chandra Mouli L, Naidu PY, Srinivasan T. Isolation and screening of lactobacillus bacteria for ability to produce antibioticsâ€. Int Lett Nat Sci 2015;5:68-76.
7. Vandana Bali, Manab Bera B, Parmjit Panesar S. Utilization of agro-industrial by products for bacteriocin production using newly isolated enterococcus faecium BS13â€. Int J Biol Veterinary Agric Food Eng 2014;8:315-28.
8. Murray PR, Baroon EJ, Pfaller MA, Tenover FC, Yolke RH. Manual of clinical microbiologyâ€. Am Soc Microbiol 1995;6:115-26.
9. Moshood AY, Tengku Haziyamin Abdul, Tengku Abdul Hamid. Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3â€. N J Pharm 2012;2:49-59.
10. Ouardy Khay, Mustapha Ouhsassi, Abdeltif El Harsal, Mohamed Idaomar, Jamal Abrini. Optimization of bacteriocin-like production by enterococcus durans E204 isolated from camel milk of moroccoâ€. Curr Res Microbiol Biotechnol 2013;1:155-9.
11. Wefky SH, Abou-Elela GM, El-Bestawy E. Optimization of fermentation conditions for bioactive compounds production by marine bacterium Enterococcus faeciumâ€. J Appl Sci Res 2009;5:1445-54.