@article{Sathe_Dighe_2016, title={HPLC METHOD DEVELOPMENT AND VALIDATION FOR QUANTITATION OF TRIGONELLINE FROM MIRABILIS JALAPA LINN. LEAVES AND ENHANCEMENT IN EXTRACTION YIELD FROM ULTRA FINE POWDER}, volume={9}, url={https://journals.innovareacademics.in/index.php/ijcpr/article/view/16611}, DOI={10.22159/ijcpr.2017v9i1.16611}, abstractNote={<p><strong>Objective: </strong>Development and validation of a simple and reliable HPLC method for determination of an alkaloid, trigonelline, in the methanolic extract of <em>Mirabilis jalapa</em> Linn. leaves and comparing the extraction yields of trigonelline from micro powder and ultrafine powder.<strong></strong></p><p><strong>Methods: </strong>The quantitation of trigonelline was carried out on a Phenomenex<strong> </strong>(Luna 5 U RP C8 (2) column, 25 cm x 4.6 mm, i.d. 5 µm), using mobile phase comprising of distilled water containing HCl (pH adjusted to 3.5) and methanol in the volume ratio of 70:30, which was delivered at the flow rate of 0.5 ml per min, at 35 °C column temperature. The detection and quantitation of trigonelline were carried out using PDA detector at the wavelength λ=264 nm.</p><p>Ultra-fine powder of M<em>irabilis jalapa</em> Linn. was prepared using simple stepwise powdering method. The dried leaves of <em>Mirabilis jalapa</em> Linn. were ground using ice jacketed domestic mixer. This powder was sieved through a BSS 85 mesh sieve and considered as a micro powder. Further fine grinding was done by jet milling, followed by ball milling. This powder was considered as an ultra-fine powder.<strong></strong></p><p><strong>Results: </strong>The proposed HPLC method for quantitation of trigonelline from dried leaf powder of <em>Mirabilis jalapa</em> Linn. is rapid, simple, accurate and precise.</p><p><strong>Conclusion: </strong>The amount of trigonelline obtained using methanolic extracts of <em>Mirabilis jalapa</em> Linn. Ultra-fine powder and the micro powder was found to be 1.1103 mg/g and 0.7258 mg/g respectively.</p>}, number={1}, journal={International Journal of Current Pharmaceutical Research}, author={Sathe, Padma S. and Dighe, Vidya V.}, year={2016}, month={Dec.}, pages={62–66} }