TY - JOUR AU - JACOB, JENSON PY - 2021/05/15 Y2 - 2024/03/29 TI - PHYTOCHEMICAL SCREENING, IN VITRO ANTIOXIDANT AND ANTI PROTEASE ACTIVITIES OF METHANOLIC EXTRACT OF NILGIRIANTHUS HEYNEANUS NEES STEM JF - International Journal of Current Pharmaceutical Research JA - Int J Curr Pharm Sci VL - 13 IS - 3 SE - Original Article(s) DO - 10.22159/ijcpr.2021v13i3.42090 UR - https://journals.innovareacademics.in/index.php/ijcpr/article/view/42090 SP - 24-27 AB - <p><strong>Objective: </strong>The aim of this study was focussed on phytochemical screening, <em>in vitro</em> antioxidant and antiprotease activities of methanolic extract of <em>Nilgirianthus heyneanus</em> stem.</p><p><strong>Methods: </strong>The stem of the plant was washed thoroughly, shade dried and coarsely powdered. The powdered material of Niligirianthus heyneanus stem was extracted with methanol using soxhlet apparatus. Preliminary phytochemical screening for carbohydrates, proteins, alkaloids, phytosteroids, flavonoids, glycosides, polyphenolics, saponins, tannins was done by following standard procedure. <em>In vitro</em> antioxidant activities of methanolic extract were assessed using DPPH, ABTS and total antioxidant capacity. <em>In vitro</em> anti-protease activity of the plant was analysed using trypsin as an enzyme and BAEE (N-benzoyl-L-arginine ethyl ester) as a substrate.</p><p><strong>Results:</strong> The results showed that phytochemicals such as carbohydrates, proteins and amino acids, flavonoids, glycosides, tannins and polyphenolics are present in the methanolic extract of <em>Niligirianthus heyneanus</em> stem. The <em>in vitro</em> antioxidant and antiprotease activities of <em>Niligirianthus heyneanus</em> stem clearly showed that the plant has antioxidant and antiprotease activity.</p><p><strong>Conclusion:</strong> From this work, it can be concluded that Niligirianthus heyneanus stem has the potential to be a strong antioxidant and protease inhibitor.</p> ER -