TY - JOUR AU - NAIR, SEEMA S. AU - VARKEY, JOYAMMA PY - 2021/09/16 Y2 - 2024/03/29 TI - ISOLATION OF PHYTOCONSTITUENT, IN VITRO ANTICANCER STUDY IN HELA AND MCF-7 CELL LINES AND MOLECULAR DOCKING STUDIES OF POTHOS SCANDENS LINN JF - International Journal of Current Pharmaceutical Research JA - Int J Curr Pharm Sci VL - 13 IS - 5 SE - Original Article(s) DO - 10.22159/ijcpr.2021v13i5.1882 UR - https://journals.innovareacademics.in/index.php/ijcpr/article/view/43153 SP - 42-51 AB - <p><strong>Objective: </strong>This study aims to isolate<strong>&nbsp;an active phytoconstituent from ethanolic leaf extract of&nbsp;</strong><em>Pothos scandens</em><strong>&nbsp;Linn., to evaluate&nbsp;</strong><em>in vitro</em><strong>&nbsp;anticancer activity, and to carry out molecular docking studies of the isolated phytoconstituent. </strong></p><p><strong>Methods: The bioactive constituent&nbsp;</strong>1,1’-(4,5-dihydroxy benzene-1,2-diyl) bisoct-7-en-1-one, a phenolic compound, was isolated by using chromatographic methods and the structure was elucidated by various spectroscopic techniques.&nbsp;<em>In vitro</em>&nbsp;anticancer activity was evaluated against HeLa and MCF 7 cell lines. The viability of cells was evaluated by direct observation of cells by an Inverted phase-contrast microscope and by the MTT assay method. IC<sub>50</sub> was calculated using the linear regression model.</p><p><strong>Results: </strong>The results of anticancer studies revealed that different concentrations of the ethanolic extract of leaves of&nbsp;<em>Pothos scandens</em>&nbsp;Linn. exhibited cytotoxic activity against HeLa and MCF 7 cell lines with IC<sub>50</sub> of 22.9 and 18.32 μg/ml, respectively. The anticancer potential of the plant was revalidated by&nbsp;<em>in silico</em>&nbsp;molecular docking study with Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, PDB ID: 4AG8) using Discovery studio 2018. Results of the docking study showed that the ligand exhibited strong interaction with the VEGFR2 kinase with significant binding energy.</p><p><strong>Conclusion: </strong><em>Pothos scandens</em>&nbsp;linn. can be used for the isolation of potent anticancer agents.</p> ER -