• Abhishek Sharma Bhupal Nobles' College of Pharmacy, Udaipur, Rajasthan.


Objective: To estimate antithrombotic effect of antiplatelet drugs, in collagen epinephrine treated swiss mice. Materials and Methods: Lung damage (pulmonary thromboembolism) in swiss mice treated with collagen (1.5 mg/kg) and epinephrine (0.5 mg/kg) by tail vein was studied by assessing parameters such as percentage protection. The effect of antiplatelet drugs (30µM oral) on above parameters was investigated .This biochemical observations were supplemented by histological examination of lung sections. The data from the dose response studies were subjected to one-way ANOVA followed by post hoc Bonferroni's Multiple Comparison test. Results: Administration of antiplatelet drugs (30µM oral) significantly increased percentage protection against collagen epinephrine induced pulmonary thromboembolism. Histological examination of lung sections revealed less thrombi generation, after administration of various antiplatelet drugs. Conclusions: The study suggests preventive action of antiplatelet drugs in collagen epinephrine induced pulmonary thromboembolism.

Author Biography

Abhishek Sharma, Bhupal Nobles' College of Pharmacy, Udaipur, Rajasthan.


1. Pozgajova M., Sachs UJH., Hein L., and Nieswandt B., Reduced Stability in mice lacking the α2A-adrenergic receptor. Blood 108, 2006: p.510–14.
2. Furie B., & Furie B.C., Mechanisms of thrombus formation. N Engl J Med 359, 2008: p.938-49.
3. Prakash, P. et al. Anti-platelet effects of Curcuma oil in experimental models of myocardial ischemia-reperfusion and thrombosis. Thromb Res 127, 111-8.
4. Raghavan S.A., Sharma P., & Dikshit M., Role of ascorbic acid in the modulation of inhibition of platelet aggregation by polymorphonuclear leukocytes. Thromb Res 110, 2003: p.117-26
5. DiMinno G., Silver M.J., Mouse antithrombotic assay: a simple method for the evaluation of antithrombotic agents in vivo. Potentiation of antithrombotic activity by ethyl alcohol. J Pharmacol Exp Ther 225, 1983: p.57-60.
6. Weiner N., Norepinephrine, Epinephrine and the sympathomimetic amines. In the pharmacological basis of therapeutics,6th ed., Gilman A.G., Goodman LS., and Gilman A., ,Macmillan publishing co., New York 138,1980
7. Inoue O., Suzuki-Inoue K., Dean W.L., Frampton J., & Watson S.P., Integrin alpha2 beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2. J Cell Biol 160, 2003: p.769-80.
8. Moroi M., & Jung S.M., Platelet glycoprotein VI: its structure and function. Thromb Res 114, 2004: p. 221-33.
9. Nieswandt B., Watson S.P., Platelet-collagen interaction: is GPVI the central receptor? Blood 102, 2003: p.449-61.
10. Andrews R.K., Gardiner E.E., Shen Y., Whisstock J.C., & Berndt M.C., Glycoprotein Ib-IX-V. Int J Biochem Cell Biol 35, 2003 : p.1170-4.
11. Fabre J.E., et al. Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice. Nat Med 5, 1999: p. 1199-202.
12. Bhatt D.L., & Topol E.J., Scientific and therapeutic advances in antiplatelet therapy. Nat Rev Drug Discov 2, 2003: p. 15-28.
13. Born G.V., Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194, 1962: p. 927-9.
14. Jackson S.P., & Schoenwaelder S.M., Antiplatelet therapy: in search of the 'magic bullet'. Nat Rev Drug Discov 2, 2003: p. 775-89.
15. O'Brien J.R., Platelet aggregation: Part I Some effects of the adenosine phosphates, thrombin, and cocaine upon platelet adhesiveness. J Clin Pathol 15, 1962: p. 446-52.
16. Born G., & Patrono C., Antiplatelet drugs. Br J Pharmacol 147 Suppl 1, 2006 S: p.241-51
17. Angelillo-Scherrer A., DE Frutos PG., Aparicio C., Melis E., Savi P., Lupu F., Arnout J., Dewerchin M., Hoylaerts M F., Herbert J-M., Collen D., Dahlbäck B., Carmeliet P., Deficiency or inhibition of Gas6 causes platelet dysfunction and protects mice against thrombosis. Nature Med 7 ,2001: p.215–221
18. Griffett EM., Kinnon SM., Kumar A., Lecker D., Smith GM., Tomich LEG Effects of 6-[p-(4-phenylacetylpiperazin-1-yl) phenyl]-4,5-dihydro-3(2H) pyridazinone CCI 17810) and cilostazol on platelet aggregation and adhesiveness. Br J Pharmacol 72,1981: p.697–705
19. Yang J., Wu J., Kowalska MA., Prevost N., O’Brien PJ., ManningD., Poncz M., Lucki I., Blendy JA., Brass LF., Loss of signaling through the G protein, Gz, results in abnormal platelet activation and altered responses to psychoactive drugs. Proc Natl Acad Sci USA 97,2000: p.9984–9989
20. Nishizawa E., Wynalda DJ., Suydam DE., Sawa TR., Schultz JR., Collagen-induced pulmonary thromboembolism in mice. Thromb Res.1,1972: p. 233–242
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How to Cite
Sharma, A. (2013). ESTIMATION EFFICACY OF ANTITHROMBOTIC ACTIVITY OF VARIOUS ANTIPLATELET DRUGS IN MURINE MODEL. Innovare Journal of Health Sciences, 1(1), 16-18. Retrieved from