CYTOTOXIC EFFECTS OF FERMENTED AFRICAN LOCUST BEAN SEEDS ON A BREAST CANCER CELL

Authors

  • RACHAEL ADERONKE AYO-LAWAL National Centre for Technology Management, P.M.B. 012, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
  • OMOLAJA OSONIYI Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • OLUWATOSIN E ILEVBARE National Centre for Technology Management, P.M.B. 012, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
  • OKOBI EKPO Department of Medical Bioscience, University of the Western Cape, Life Sciences Building, Private Bag Box X17, Bellville, Cape Town 7535, South Africa.

Keywords:

Fermented African locust bean seeds, Breast cancer cell, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Cytotoxicity, iru

Abstract

Objective: There is a growing scientific evidence of the health-enhancing benefits of fermented food especially in cancer treatment and prevention. Fermented African locust beans (FALB) are a condiment with many medicinal activities and consume in many West African countries. Breast cancer is the most common cancer among women globally. This study investigated the cytotoxicity of FALB extracts on breast cancer (MCF-7) cells overtime and at different concentrations.

Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to quantify the extent of cytotoxicity of the extracts.

Results: The aqueous extract of FALB had an half maximal inhibitory concentration (IC50) of 1.51 and 0.98 mg/mL on exposure for 24 and 48 h, respectively, against MCF-7 cells. Comparatively, the IC50 obtained for the same extract against normal human fibroblasts was 1.90 and 1.37 mg/mL, respectively.

Conclusion: The results obtained here suggest some measure of selective cytotoxicity by the aqueous extract against transformed as compared with normal cells. These findings present an important lead to the usefulness of this condiment in cancer treatment. Further studies are recommended.

Author Biographies

OMOLAJA OSONIYI, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.

Department of Biochemistry and Molecular Biology

Obafemi Awolowo University

Ile-Ife, Osun State

OLUWATOSIN E ILEVBARE, National Centre for Technology Management, P.M.B. 012, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.

Science Policy and Innovation Studies Department,

National Centre for Technology Management

OKOBI EKPO, Department of Medical Bioscience, University of the Western Cape, Life Sciences Building, Private Bag Box X17, Bellville, Cape Town 7535, South Africa.

Department of Medical Bioscience, University of the Western Cape, Life Sciences Building, Private Bag Box X17, Bellville, Cape Town 7535, South Africa

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Published

01-05-2020

How to Cite

AYO-LAWAL, R. A., OSONIYI, O., E ILEVBARE, O., & EKPO, O. (2020). CYTOTOXIC EFFECTS OF FERMENTED AFRICAN LOCUST BEAN SEEDS ON A BREAST CANCER CELL. Innovare Journal Health Sciences, 8(3), 1–4. Retrieved from https://innovareacademics.in/journals/index.php/ijhs/article/view/36935

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