EASY AND EFFICIENT SPLIT-ROOT METHOD TO STUDY MORPHOLOGY AND ANATOMY OF RICE (Oryza sativa L.)


Raveendra G M, Rukaya Amin Chowdery, Shashidhar H E

Abstract


ABSTRACT:

Objective: This study was intended to investigate the efficacy of PVC pipe method for split-root experiment in rice (Oryza sativa L.) using soil medium at all the growth stages of rice.

Methods: Rice seeds were sown in small PVC pipes and allowed to grow for one month, which were then transferred to split-root setup by equally splitting the roots of these seedlings into two halves and were allowed to grow for different time periods of their growth stages to analyze their health and stability.

Results: We report an easy split-root study for rice grown in soil. Unlike the field grown plants, the efficient PVC tube method enables simple and systematic growth and harvesting for proper analysis of the plant samples without damaging the tissue. In our experiments, although the rice plants were transferred to the split-root setup by splitting their roots, they were healthy and stable after 7days, 15 days, 70 days and even at 120 days (maturity) of growth in split-root condition.

Conclusion: Morphology and anatomy of plants can be easily and efficiently studied at any growth stage using PVC tube method as opposed to field method where sample harvesting requires inconvenient process of uprooting the plant while losing and damaging the tissue.


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About this article

Title

EASY AND EFFICIENT SPLIT-ROOT METHOD TO STUDY MORPHOLOGY AND ANATOMY OF RICE (Oryza sativa L.)

Date

01-10-2016

Additional Links

Manuscript Submission

Journal

Innovare Journal of Life Sciences
Vol 4 Issue 5 2016 (Oct-Nov) Page: 10-12

Online ISSN

2321-550X

Statistics

63 Views | 179 Downloads

Authors & Affiliations

Raveendra G M
Bioseed Research India Pvt. Ltd. ICRISAT, Patancheru, Telangana, India.
India

Rukaya Amin Chowdery
National Centre for Biological Sciences, Bangalore, India. Manipal University, Manipal, India
India

Shashidhar H E
Department of Plant Biotechnology, University of Agricultural Sciences, Bangalore, India.
India


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