ANTIOXIDANT, ANTI-INFLAMMATORY AND DIABETES RELATED ENZYME INHIBITION PROPERTIES OF LEAVES EXTRACT FROM SELECTED VARIETIES OF PHOENYX DACTYLIFERA L.
Objective: to investigate the antioxidant, anti-inflammatory, and antidiabetic activities of ethanolic leaves extracts of three selected varieties of Phoenyx dactylifera L. namely: â€œGharsâ€, â€œDeglet Nourâ€ and â€œHamrayaâ€. Methods: The assessment of the antioxidant potential of crude leaves extracts, using superoxide anions inhibition, DPPH and total antioxidant activity essays, was carried out. Furthermore, the anti-inflammatory properties of the extracts were determined by measuring the inhibition of nitric oxide (NO) production. Moreover, the antidiabetic effect was evaluated by inhibition of Î±-amylase and Î±-glucosidase enzymes. The total phenolic content measured by Folin-ciocalteu method was as well conducted. Results: The raw leaves extracts of the selected varieties was found to contain a high content of total phenolic content (342.45 mg GAE/ gDW for GE) and therefore exhibited a higher antioxidant activity and inhibitory effect of radicals scavenging activity against DPPH and superoxide anion (IC50=7.44 Î¼g/mL and 39.11 Î¼g/mL respectively). The three varieties exhibited significant anti-inflammatory effects using in-vitro inhibition of NO (IC50=240.28 Î¼g/mL for GE). The extracts also displayed high inhibition actions against Î±-amylase. Conclusion: the results suggest that the leaves of the three selected varieties of Phoenyx dactylifera can be considered as a good source of natural antioxidant and anti-inflammation drugs as well as potent antidiabetic medicine.
 Dowson VHW. Date protection and production. FAO Plant protection and Production Paper No.35. Food and Agriculture Organisation of the United Nations. Rome (1982).
 Baliga MS, Vitttaldas Baliga BR, Kandathil SM, Bhat HP, Vayalil PK. A review of the chemistry and pharmacology of the date fruits (Phoenis d'actylifera L.). Food Res Int 2011; 44:1812-1822.
 Al-Shahib W, Marshall RJ. The fruit of the date palm: Its possible use as the best food for the futur. Int J Food Sci. Nutr 2003;54:247-259.
 Chandra M, Riley MG, Johnson DE. Spontaneous neoplasms in aged Sprague-Dawley rats. Arch Toxicol 1992;66:496-502.
 Ahmed AI, Ahmed AWK, Robinson RK. Chemical composition of date varieties as influence by the stage of repening. Food Chem 1995; 45:305-309.
 Thomas M, Emilie D, GaÃ«tan Le F, Marie EL, Claire E. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (HippophaÃ« rhamnoides L.) leaf, stem, root and seed. Food Chem 2012;131. 754â€“760.
 Rubem CH, Vera Maria FV. Antimutagenic activity of extracts of natural substances in the Salmonella/microsome assay. Mutag 2003;18:113-118.
 Hossain MA, Rahman SMM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res. Int 2011;44: 672-676.
 Pandhair V, Sekhon BS. Reactive oxygen species and antioxidants in plants: an overview. J. Plant Biochem. Biotechnol. 2006; 15:71-78
 Mayer R, Stecher G, Wuerzner R, Silva RC, Sultana T, Trojer L, et al. Proanthocyanidins: Target compounds as antibacterial agents. J.Agri Food Chem 2008;56:6959-6966.
 Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, et al. Antimicrobial natural products: An update on future antibiotic drug candidates. Nat Prod Rep 2012;27:238-254.
 Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000, 63;1035:1042.
 Rice-Evans CA, Miller NJ, Pacanga G. Antioxidant properties of phenolic compounds. Trens plant Sci 1997; 2:152-159.
 Edziri H, Ammar S, Souad L, Mahjoub MA, Mastouri M, Aouni M, et al. In vitro evaluation of antimicrobial and antioxidant activities of some Tunisian vegetables. S. Afr. J. Bot 2012;78.252-256.
 Maria A, Harris P, Dimitris K, Alexio-Leandros S, Serkos AH. Grape stem extracts: polyphenolic content ans essessment of their in vitro antioxidant properties. Food Sci.Technol Int 2012;48:316-322.
 Proestos C, Sereli D, Komaitis M. Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS. Food Chem 2006;95:44-52.
 Terao K, Ohmori S, Igarashi K, Ohtani I, Watanabe MF, Harada KI, et al. Electron microscopic studies on experimental poisoning in mice induced by cylindrospermopsin isolated from blueâ€“green alga Umezakianatans. Toxicon 1994;32:833-843.
 Sweetie RK, Arjun K, Arun S. Antioxidant and Antimicrobial activity of legume hulls. Food Res Int 2011;44:3182-3187.
 Sunday OO, Anthony JA. Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pac J Trop Med 2011;4: 952-958
 Neha B, Harinder SO, Dewinder SU, Ramabhau TP. Total phenolic content and antioxidant capacity of extracts obtained from six important fruit residues. Food Res Int 2011;44:391:396.
 Jonathan DA, Esther GS, Esperanza VS, David GG. In vitro estimation of the antibactarial activity and antioxidant capacity of aqueous extracts from grape-seed (Vitis vinifera L). Food Control 2012;24:136-141.
 Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdicâ€“phosphotungstic acid reagents. Am J Enol.Viticult 1965;16.144-158.
 Shaofang L, Jie S, Lina Y, Chushu Z, Jie B, Feng Z, et al. Antioxidant activity and phenolic compounds of Holotrichia parallela Motschulsky extracts. Food Chem 2012;134:1885-1891.
 Hanato T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem. Pharma. Bull 1988;36: 2090-2097.
 Falleh H, Ksouri R, Oueslati S, Guyot S, MagnÃ© C, Abdelly C. Interspecific variability of antioxidant activities and phenolic composition in Mesembryanthemum genus. Food. Chem Toxicol 2009;47:2308-2313.
 Kilani-Jaziri S, Bhouri W, Skandrani I, Limem I, Chekir-Ghedira L, Ghedira K. Phytochemical, antimicrobial, antioxidant and antigenotoxic potentials of Cyperus rotundus extracts S Afr J. Bot 2011;77:767-776
 Praveen K Vayalil. Antioxidant and antimutagenic properties of aqueous extract of date fruits (Phoenix dactylifera L. Arecaceae). J. Agric. Food Chem 2002;50:610-617.
 Christudas S, Paul A, Chidambarm K, Savarimuthu I. In vitro antioxidant, antidiabetic and antilipidemic activities of Symplocos cochinchinensis (Lour) S. Moore bark. Food. Chem Toxicol 2012;50;1547-1553.
 Mbaebie BO, Edeoga HO, Afolayan AJ. Phytochemical analysis and antioxidants activities of aqueous stem bark extract of Schotia latifolia Jacq. Asian Pac J Trop Biomed 2012;2:118-124.
 Qing-Feng Z, Yu-Xian G, Xinchen S, Guodong Z, Wen-Jun W. Antioxidant and anti proliferative activity of Rhizoma Smilacis Chinae extracts and main constituents. Food Chem 2012;133: 140-145.
 Catherin NK, Jasper KI, Michael WO,Hans KB. Total phenolic content, antioxidant and antidiabetic of methanolic extract of raw and traditionally prossed Kenyan indigenous food ingredients. Food Sci. Technol 2012;45:269-276.
 Dhaouadi K, Raboudi F, Estevan K, Barrajon E, Vilanova E, Hamdaoui M, et al. Cell Viability Effects and Antioxidant and Antimicrobial Activities of Tunisian Date Syrup (Rub El Tamer) Polyphenolic Extracts. J Agric. Food Chem 2011;59:402-406.
 Manjeshwar SB, Bantwal Raghavendra VB, Shaun MK, Harshith PB, Praveen KV A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Int 2011;44:1812-1822
 Chaira N,Smaali MI,Martinez-Tome M,Mrabet A, Murcia MA,Ferchichi A. Simple phenolic composition, flavonoid contents and antioxidant capacities in waterâ€“methanol extracts of Tunisian common date cultivars (Phoenix dactylifera L.). Int J. Food Sci. Nutr 2009;60:316-329.
 Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). J Ethnopharmacol 2007;110:105-117.
 Doha MA, Al-Okbi SY. In vivo evaluation of antioxidant and anti-inflammatory activity of different extracts of date fruits in adjuvant arthritis. Pol. J. Food Nutr. Sci 2004;13:397:402.
 Loganayaki N, Siddhurajub P, Manian S. Antioxidant, anti-inflammatory and anti-nociceptive effects of Ammannia baccifera L. (Lythracceae), a folklore medicinal plant. J Ethnopharmacol 2012;140:230-233.
 Ruch RJ, Cheng SJ,Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechin isolated from Chinese green tea. Carcinogens 1989;10:1003-1008.
 Motar MLR, Thomas G, Barbose Fillo JM. Effect of Anacardium occidentale stem bark extract on in vivoinflammatory models. J Ethnopharm 1985;95:139-142.
 Olayinka A A, Anthony IO. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. 2010;10/21:1-8.
 Edenharder R, Grunhage D. Free radical scavenging abilities of flavonoids as mechanism of protection against mutagenicity induced by tert-butyl hydroperoxide or cumene hydroperoxide in Salmonella typhimurium TA102. Mutat. Res 2003;540:1-18.
 Park KY, Jung GO, Lee KT, Choi J, Choi MY, Kim GT, et al. Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J Ethnopharmacol 2004; 90:73-79.
 Indrajit K, Narayan D, Pathik Saha, Nilanjan S, Asis B, Pallab KH. Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Orient Pharm Exp Med 2011;11:221â€“228
 Kundu S, Bala A, Ghosh P, Mukhopadhya D, Mitra A, Sarkar A, et al. Attenuation of oxidative stress by allylpyrocatechol in synovial cellular infiltrate of patients with rheumatoid arthritis. Free Radic Res 2011;45:518â€“526.
 Duenas M, Hernandez T, Estrella I. Assessment of in vitro antioxidant capacity of the seed coat and the cotyledon of legumes in relation to their phenolic contents. Food Chem 2006;98, 95-103.
 Jiang F, Dusting GJ. Natural phenolic compounds as cardiovascular therapeutics: potential role of their anti-inflammatory effects. Curr. Vasc. Pharmacol 2003;1;135-156.
 Randhir R, Kwon YI, Shetty K. Effect of thermal processing on phenolics, antioxidant activity and health-relevant functionality of select grain sprouts and seedlings. Innovat Food .Sci Emerg Tech, 2008;9:355-364.
 Chethan S, Sreerama YN, Malleshi NG. Mode of inhibition of finger millet malt amylases by the millet phenolics. Food Chem 2008;111:187-191.
 Qi Y, Feng C, Xi W, Yueming J, Songyi L. Anti-diabetic activities of phenolic compounds in muscadine against alpha-glucosidase and pancreatic lipase. LWT - Food Sci. Technol 2012;46:164-168.