PROTECTIVE EFFECT OF ABUTILON INDICUM L. (MALVACEAE) AGAINST ACETAMINOPHEN INDUCED NEPHROTOXICITY IN RATS
Acetaminophen overdose can cause nephrotoxicity with oxidative stress as one of the possible mechanisms. The effects of ethanolic extract of Abutilon Indicum[200 mg per kg of body weight (mg/kg) and 400 mg/kg] on Acetaminophen induced nephrotoxicity were evaluated. Rats were divided into five groups containing 6 rats each. The control group received distilled water while other groups were treated with extract alone (400 mg/kg), Acetaminophen alone (750 mg/kg), 750 mg/kg Acetaminophen+200 mg/kg extract (Acetaminophen+ 200-extract), and 750 mg/kg Acetaminophen+400 mg/kg extract (Acetaminophen+400-extract), respectively, for seven consecutive days. TheÂ EEAI was given orally concurrent with oral administration of Acetaminophen Treatment withÂ EEAI at doses of 200 and 400 mg/kg prevented the Acetaminophen-induced nephrotoxicity and oxidative impairments of the kidney, as evidenced by a significantly reduced (P<0.05) level of Serum creatinine, BUN, serum alkaline phosphatase, Serum uric acid, serum total proteins and total cholesterol. The nephroprotective effects ofÂ EEAI were confirmed by a reduced intensity of renal cellular damage, as evidenced by histological findings. Moreover,Â EEAI administered at 400 mg/kg was found to show greater protective effects than that at 200 mg/kg. In conclusion, EEAI has a protective role against Acetaminophen-induced nephrotoxicity and the process is probably mediated through its antioxidant properties.
Key words: Antioxidants, Cisplatin, nephrotoxiciity, Abutilon indicum
RaviR.,RiteshJ.,NarendraM.,PrshantA.,KhadbadiS.S.AntinflammatoryAction ofAbutilonindicum(L.)SweetLeavesbyHRBCMembraneStabilization.Research J Pharm and Tech. 2009;2(2):415-416.
FinleyR.S, Fortner C.L, GroveW.R.Cisplatin nephrotoxicity: a summaryofpreventativeinterventions.DrugIntellClinPharm.1985; 19:362-367.
Adelstein,D.J,LavertuP,SaxtonJ.P,SecicM,WoodB.G,WanamakerJ.R,EliacharI, Strome M, LartoM.A.Mature results of a phase IIIrandomizedtrialcomparingconcurrentchemoradiotherapywithradiationtherapyaloneinpatientswithstageIIIandIVsquamouscellcarcinomaoftheheadandneck.Cancer 2000; 88:876-883.
Jiang M,Wei Q, Pabla, N, Dong G, Wang C.Y, Yang, T, Smith S.B, Dong Z. Effects of hydroxyl radical scavenging on cisplatin-induced p53 activation, tubular cell apoptosis and nephrotoxicity. BiochemPharmacol. 2007; 73: 1499â€“1510.
SammiaY,MuhammadA.K,MuhammadN.A,MushtaqA,AyeshaMohy-ud-Din. Antioxidant potential and radical scavenging effects of various extracts from Abutilon indicumand Abutilon muticum. Pharmaceutical Biology2010;48(3):282-289.
Ghaisas MM, Navghare VV, Takawale AR, Zope VS, Phanse MA. Antidiabetic and Nephroprotective effect of TectonagrandisLinn. In Alloxan induced Diabetes. Ars Pharm. 2010; 51(4):195-206.
Golwala D.K, Patel L.D, Vaidyal S.K, Bothara1 S.B, MuneshM, Patel P. AnticonvulsantactivityofAbutilon indicumleaf.InternationalJournalofPharmacyand Pharmaceutical Sciences 2010; 2(1):66-71.
Kakasaheb J. Kore, Dr. Rajkumar V. Shete, Apsari J. Patel. Effect of Abutilon indicumExtract in Gentamicin Induced Nephrotoxicity. International Journal of Pharmaceutical Research & Development 2011; 3(7): 73-79.
Yasmin S, Kashmiri MA, Asghar MN, Ahmad M, Mohy-ud-Din A., Antioxidant potential and radical scavenging effects of various extracts from Abutilon indicum and Abutilonmuticum. Pharm Biol. 2010;48(3):282-9.
Gaind KN, Chopra KS., Phytochemical investigation of Abutilon indicum; Planta Med. 1976; 30(2):174-88.
Chandrashekhar V.M, Nagappa A.N, ChanneshT.S. Antidiarrhoealactivity of Abutilon indicumLinnleafextract in rats.J Nat Remedies 2004;4(10):12-16.
Tripathi P, Chauhan NS, Patel JR., Anti-inflammatory activity of Abutilon indicum extract. Nat Prod Res. 2011 Oct 14.
Ahmed M, Amin S, Islam M, Takahashi M, Okuyama E, Hossain CF., Analgesic principle from Abutilon indicum. Pharmazie. 2000; 55(4):314-6.
Antunes L.M.G, Darin J.D.C, Bianchi, M.L.P, Effects of the antioxidants curcumin or selenium on cisplatin-induced nephrotoxicity and lipid peroxidation in rats. Pharmacol. Res. 2001; 43: 145â€“150.
Corcostegui R, Labeaga L, Arteche JK, Orjales A. Protective effect of hidrosmin against cisplatin induced acute nephrotoxicity in rats. J Pharm Pharmacol. 1998; 4: 465-467.
Murthy R.L.N, Nataraj H.N, RamachandraSetty S. Nephroprotective activity of CyanotisFasiculata var., Fasiculata against cisplatin induced Nephrotoxicity. IJRP. 2011; 2(9):137-142.
BartelinkH, SchellensJ.H.M, VerheijM.The combined use ofradiotherapy and chemotherapy in the treatmentof solidtumours.EurJCancer 2002; 38: 216-222.
MatÅ‚awska I, Sikorska M. Flavonoid compounds in the flowers of Abutilon indicum (L.) Sweet (Malvaceae). Acta Pol Pharm. 2002;59(3):227-9.
NishantaR,CoryS,Harris,G.H.N.Towers.AntimicrobialActivityofPlants CollectedfromSerpentineOutcropsinSriLanka.PharmaceuticalBiology2002;40(3): 235â€“244.
E.Porchezhian, Ansari S.H.Hepatoprotective activity of Abutilon indicum on experimental liver damage in rats.Phytomedicine: International Journal of Phytotherapy&Phytopharmacology 2005; 12: 62-64.
Yang J, Meyers K, Van der Heide J & Liu RH. Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions. J Agri and Food Chem. 2004; 52: 6787â€“6973.
Yao, X., Panichpisal, K., Kurtzman, N., Nugent, K., Cisplatin nephrotoxicity: a review. Am. J. Med. Sci. 2007; 334:115â€“124.
ChutwadeeKrisanapun,Seong-Ho Lee,PenchomPeungvicha,RungraviTemsiririrkkul,and SeungJoonBaek. Antidiabetic Activities of Abutilon indicum (L.) Sweet Are Mediated by Enhancement of Adipocyte Differentiation and Activation of the GLUT1 Promoter. Evid Based Complement Alternat Med. 2011; 167- 684.