• NEHA GUPTA School of Studies in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India.
  • SAMEER SURESH BHAGYAWANT School of Studies in Biotechnology, Jiwaji University, Gwalior, Madhya Pradesh, India.




Kabuli chickpea, Inter simple sequence repeat-PCR, Plant breeding, MIPS gene, Chickpea apoptosis


Objective: Chickpea is an annual legume known for its high seed protein thus beneficial for one’s health and well-being. Of the two chickpea seed types, Kabuli seeds have reasonably high-protein and -carbohydrate contents, as a result, set up more commercial demand.

Methods: Inter simple sequence repeat (ISSR) markers are useful in the areas of genetic diversity. The present study attempted ISSR-PCR experimentation in testing DNA variation predominantly in Kabuli chickpea seed accessions employing 10 different set of ISSR primers.

Results: In vitro amplification by PCR revealed an average number of bands per primer per accession which accounted to 8.7. Maximum number of bands (25) scored by the primer UBC-836 while least number of bands (12) were displayed by UBC-811.

Conclusion: The accession JGK-1 and IPCK-12-288 presented divergent DNA polymorphic among all chickpea accessions tested and therefore found distinct. The genetic divergence at DNA level existed between these chickpea accessions can be used proficiently for future plant breeding program.


Maesen van der LJ. Origin, history and taxonomy of chickpea. In: Saxena MC, Singh KB, editors. The Chickpea. Wallingford, Oxfordshire: CAB International; 1987. p. 11-34.

Gupta N, Shrivastava N, Bhagyawant SS. Multivariate analysis based on nutritional value, antinutritional profile and antioxidant capacity of forty chickpea genotypes grown in India. J Nutr Food Sci 2017;7:3.

Gupta N, Bhagyawant SS. Bioactive peptide of Cicer arietinum L. induces apoptosis in human endometrial cancer via DNA fragmentation and cell cycle arrest. 3 Biotech 2021;11:63.

Bhagyawant SS, Narvekar DT, Gupta N, Bhadkaria A, Gautam AK, Srivastava N. Chickpea (Cicer arietinum L.) lectin exhibit inhibition of ACE-I, α-amylase and α-glucosidase activity. Protein Pept Lett 2019;26:494-501.

Bhagyawant SS, Gautum AK, Narvekar DT, Gupta N, Bhadkaria A, Srivastava N, et al. Biochemical diversity evaluation in chickpea accessions employing mini-core collection. Physiol Mol Biol Plants 2018;24:1165-83.

Bhagyawant SS, Srivastava N. Assessment of antinutritional factors and protein content in the seeds of chickpea cultivars and their divergence. J Cell Tissue Res 2008;8:1333-8.

Bhagyawant SS, Gupta N, Srivastava N. Biochemical analysis of chickpea accessions vis-a-vis; zinc, iron, total protein, proline and antioxidant activity. Am J Food Sci Technol 2015;3:158-62.

Bhagyawant SS. Antinutritional factors in pulses and their diversity. Innov J Food Sci 2013;1:27-9.

Bhagyawant SS, Shrivastava N. Recent Advances in Plant Molecular Biology. Vol. 11. Mumbai: Himalaya Publishing House; 2019.

Singh PK, Gautam AK, Akare UR, Bhagyawant SS. Studies on enzymatic changes and their impact on antinutritional factors of pulses during germination. Biosci Biotechnol Res Commun 2014;7:27-31.

Singh PK, Shrivastava N, Sharma B, Bhagyawant SS. Effect of domestic processes on chickpea seeds for antinutritional contents and their divergence. Am J Food Sci Technol 2015;3:111-7.

Bhagyawant SS, Gupta N, Shrivastava N. Effects of gamma irradiation on chickpea seeds vis-a-vis total seed storage proteins, antioxidant activity and protein profiling. Cell Mol Biol 2015;61:79-83.

Gautam AK, Shrivastava N, Sharma B, Bhagyawant SS. Current scenario of legume lectins and their practical applications. J Crop Sci Biotechnol 2018;21:217-27.

Peumans WJ, Van Damme EJ. Lectins as plant defense proteins. Plant Physiol 1995;109:347-52.

Gautam AK, Gupta N, Narvekar DT, Bhadkaria A, Kumari S, Srivastava N, et al. Chickpea lectin: Purification strategies, characteristics and potential applications. In: Hemagglutinins Structures, Functions and Mechanisms. New York: Nova Science Publishers, Inc.; 2019. p. 107-33.

Gupta N, Gautam AK, Bhagyawant SS. Biochemical characterization of lectin from wild chickpea (Cicer reticulatum L.) with potential inhibitory action against human cancer cells. J Food Biochem 2018;43:e12712.

Gautam AK, Gupta N, Narvekar DT, Bhadkariya R, Bhagyawant SS. Characterization of chickpea (Cicer arietinum L.) lectin for biological activity. Physiol Mol Biol Plants 2018;24:389-97.

Gautam AK, Srivastava N, Chauhan AKS, Bhagyawant SS. Analysis of wild chickpea seed proteins for lectin composition. Int J Curr Res Aca Rev 2017;5:8-14.

Gautam AK, Srivastava N, Nagar DP, Bhagyawant SS. Biochemical and functional properties of a lectin purified from the seeds of Cicer arietinum L. 3 Biotech 2018;8:272.

Katre UV, Gaikwad SM, Bhagyawant SS, Deshpande UD, Khan MI, Suresh CG. Crystallization and preliminary X‐ray characterization of a lectin from Cicer arietinum (chickpea). Acta Crystallo F 2005;61:141-3.

Bhagyawant SS, Bhadkaria A, Gupta N, Srivastava N. Impact of phytic acid on nutrient bioaccessibility and antioxidant properties of chickpea genotypes. J Food Biochem 2018;42:e12678.

Gupta N, Bisen PS, Bhagyawant SS. Chickpea lectin inhibits human breast cancer cell proliferation and and induces apoptosis through cell cycle arrest. Protein Pept Lett 2018;25:492-9.

Bhagyawant SS, Gupta N, Gautam A, Chaturvedi SK, Shrivastava N. Molecular diversity assessment in chickpea through RAPD and ISSR markers. World J Agric Res 2015;3:192-7.

Gautam AK, Gupta N, Bhadkariya R, Srivastava N, Bhagyawant SS. Genetic diversity analysis in chickpea employing ISSR markers. Agrotechnology 2016;5:1000152.

Singh PK, Sharma H, Srivastava N, Bhagyawant SS. Analysis of genetic diversity among wild and cultivated chickpea genotypes employing ISSR and RAPD markers. Am J Plant Sci 2014;5:676-82.

Yadav P, Koul KK, Shrivastava N, Mendaki MJ, Bhagyawant SS. DNA polymorphisms in chickpea accessions as revealed by PCR-based markers. Cell Mol Biol 2015;61:84-90.

Yadav P, Koul KK, Shrivastava N, Mendaki MJ, Bhagyawant SS. ITS-PCR deciphers molecular phylogeny in chickpea. Plant Biosystems 2016;151:429-435.

Bhadkaria A, Gupta N, Narvekar DT, Bhadkariya R, Saral A, Srivastava N, et al. ISSR-PCR approach as a means of studying genetic variation in moth bean (Vigna aconitifolia (Jacq.) Maréchal), Biocata Agric Biotechnol 2020;30:101827.

Bhagyawant SS. RAPD-SCAR markers: An interface tool for authentication of traits. J Biosci Med 2015;4:1-9.

Bhagyawant SS, Srivastava N. Genetic fingerprinting of chickpea (Cicer arietinum L.) Germplasm using ISSR markers and their relationships. Afr J Biotechnol 2008;7:4428-31.

Singh M, Malhotra N, Singh, K. Broadening the genetic base of cultivated chickpea following introgression of wild Cicer species-progress, constraints and prospects. Genet Resour Crop Evol 2021;68:1173.

Bhagyawant SS, Srivastava N, Koul KK. Diversity in chickpea and its wild relatives based on seed protein profiles. Crop Res 2008;37:168-73.

Singh PK, Kumar A, Srivastava N, Agarwal RM, Bhagyawant SS. Association of protein profiling and agronomic traits in chickpea as revealed using SDS-PAGE. J Cell Tissue Res 2012;12:3279.

Singh PK, Shrivastava N, Chaturvedi K, Bhagyawant SS. Characterization of seed storage proteins from chickpea using 2D electrophoresis coupled with mass spectrometry. Biochem Res Int 2016;2016:1049462.

Gupta N, Bhagyawant SS. Impact of hydrolysis on functional properties, antioxidant, ACE-I inhibitory and anti-proliferative activity of Cicer arietinum and Cicer reticulatum hydrolysates. Nutrire 2019;44:5.

Gupta N, Bhagyawant SS. Enzymatic treatment improves ACE-I inhibiton and antiproliferative potential of chickpea. Vegetos 2019;32:363-9.

Gupta N, Bhagyawant SS. Angiotensin-I converting enzyme (ACE-I) inhibitory and antiproliferative potential of chickpea seed protein hydrolysate. Ann Plant Sci 2018;7:2149-53.

Gautam AK, Gupta N, Srivastava N, Bhagyawant SS. Proteomic analysis of chickpea roots reveal differential expression of abscisic acid responsive proteins. J Food Biochem 2019;43:12838.

Bhagyawant SS, Narvekar DT, Gupta N, Bhadkaria A, Koul KK, Srivastava N. Variations in the antioxidant and free radical scavenging under induced heavy metal stress expressed as proline content in chickpea. Physiol Mol Biol Plants 2019;25:683-96.

Gupta N, Srivastava N, Singh PK, Bhagyawant SS. Phytochemical evaluation of moth bean (Vigna aconitifolia L.) seeds and their divergence. Biochem Res Int 2016;2016:3136043.

Gupta N, Srivastava N, Bhagyawant SS. Vicilin-a major storage protein of mungbean exhibits antioxidative potential, antiproliferative effects and ACE inhibitory activity. PLoS One 2018;13:e0191265.

Bhadkaria A, Srivastava N, Bhagyawant SS. A prospective of underutilized legume moth bean (Vigna aconitifolia (Jacq.) Marechàl): Phytochemical profiling, bioactive compounds and in vitro pharmacological studies. Food Biosci 2021;42:101088.

Talebi R, Fayaz F, Mardi M, Pirsyedi SM, Naji AM. Genetic Relationships among Chickpea (Cicer arietinum) elite lines based on RAPD and agronomic markers. Int J Agri Biol 2008;10:301-5.

Jaccard P. Nouvelles researches Sur ladistribution florale. Bull Soc Vaud Sci Nat 1908;44:223-70.

Tahir NA, Karim HF. Determination of genetic relationships among some varieties of chickpea (Cicer arietinum L.) in Sulaimani by RAPD and ISSR markers. Jordan J Biol Sci 2011;4:77-86.

Rao AS, Rani PU, Deshmukh PS, Kumar PA, Panguluri SK. RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum L. Genet Resour Crop Evol 2007;54:1235-44.

Iruela M, Rubio J, Cubero JI, Gil J, Milan T. Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theor Appl Genet 2002;104:643-51.



How to Cite

GUPTA, N., & BHAGYAWANT, S. S. (2021). TESTING DNA VARIATION IN KABULI CHICKPEA SEEDS THROUGH ISSR-PCR. Innovare Journal of Life Sciences, 9(4), 1–4. https://doi.org/10.22159/ijms.2021.v9i4.42229



Original Article(s)