PREPARATION OF CHITOSAN STABILIZED OFLOXACIN- GOLD NANO CONJUGATE FOR THE IMPROVED ANTI BACTERIAL ACTIVITY AGAINST HUMAN PATHOGENIC BACTERIA
Objective .In the present study, biocom[patible polymer chitosan Â stabilized ofloxacin- gold nanoconjugate was Â synthesizedÂ for the improved anti bacterial activity against clinical isolate ofÂ E.coli and Staphylococcus aureus
Methods. Chitosan stabilized oflaxacin-gold nanoparticles conjugate was prepared with aqueous solution of ofloxacin ,gold nanoparticles suspensionÂ and chitosan under optimum condition. Synthesized nanoconjugate was .characterized byÂ scanning electron microscopy, FTIR and the synthesized nanoconjugate was tested against E.coli and Staph.aureus adopting well diffusion assay.
Result. Synthesis of chitosan stabilized ofloxacin- gold nanoconjugate was primarly confirmed by colour change of the reaction mixture, characteristic change in the FTIR pattern andÂ size and shape by SEM. Anti bacterial activity revealed increased spectrum of antibacterial activity was recorded in chitosan stabilized ofloxacin - gold nanoconjugate against both the tested strains.Â The highest increase in inhibitory zone for E.coli and Staph.aureus was observed.
Conclusion. The present study suggests possible utilization of antibiotics â€“gold nanoconjugate as an effective anti microbial agent against the pathogenic bacteria
Key words. chitosan,ofloxacin,nanoconjugate ,E.coli, Staphylococcus aureus
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