Prenatal and postnatal effect of lead acetate on the histology of frontal cortex and motor activity in Wistar rats

Authors

  • Saleh Nuhu Yusuf Maitama Sule University

Keywords:

Lead, neurodevelopment, prenatal, postnatal, motor activity

Abstract

Background/aim: The frontal lobe is the part of the brain that controls important cognitive skills in humans, such as emotional expression, problem solving, memory, language, judgment, and sexual behaviors. Exposure of Wistar rats to lead (Pb) has been shown to have effects on the CNS, kidneys, bone marrow and other body systems. Therefore, this study was conducted in order to find the effect of lead on prenatally and postnatally exposed Wistar rats.

Methods: In this study, twelve (12) Wistar Rats were used and grouped into four groups of 2 females and 1 male. Group I rats served as the control andallowedfeed and water freely.  The rats in Group II were administered 500ppm of Pb through drinking water from gestation day 8 (GD8) to parturition (GD21). While, Group III rats were given 500ppm of Pb in drinking water from postnatal day 1 (PND1) to PND21. The rats in thefourth group (Group IV) were given 500ppm of Pb from GD8 to PND21. Palmer grasp reflex was conducted to assess the motor activity of the rat pups. The animals were then humanely sacrificed and thefrontal cortices were isolated for routine histological processing.

Results: The histological study has shown normal neurons in control group while degenerating cells exhibiting karyolysis, pyknosis, karyorrhexis, vacuolation were seen in the lead-treated groups. Group II and Group IV showed considerate deficit in their motor activity Group III mild effect.

Conclusion: From this study, lead exposure of Wistar rats at both prenatal and postnatal period of development has effect on the histology of the frontal cortex as well as on their motor activity.

References

NaqiSZ. A Comparative Study Of The Histological Changes In Cerebral Cortex , Hippocampus , Cerebellum , Pons & Medulla Of The Albino Rat Due To Lead Toxicity 2015, 3(2), pp. 1173–1178.

Aprioku JS, Siminialayi IM, Harcourt P.Maternal lead exposure and pregnancy outcome in Wistar albino rats 2013; 5(10), pp. 185–193.

Adikwu E,DeoO, Geoffrey 0P, Enimeya DA.Lead Organ and Tissue Toxicity : Roles of Mitigating Agents ( Part 1) 2013; 4(6), pp. 232–240.

Thurtle N. Description of 3,180 Courses of Chelation with Dimercaptosuccinic Acid in Children with Severe Lead Poisoning in Zamfara , Northern Nigeria : A Retrospective Analysis of Programme Data 2014; 11(10).

Mo I. Histopathological Changes On The Hippocampus Of Adult Wistar Rats Exposed To Lead Acetate And Aqueous Extract Of Psidium Guajava Leaves 2015;, 31, pp. 26–31.

Bazrgar M, Abrari K,Salmani ME, Effect of Postnatal Chronic Lead Exposure on Spatial Learning and Memory in Male Rat2015; 17(9).

Ghareeb DA, Hussien HM, Khalil AA, Toxicological & Environmental Chemistry Toxic effects of lead exposure on the brain of rats : Involvement of oxidative stress , inflammation, acetylcholinesterase, and the beneficial role of flaxseed extract’, Toxicology & Enviromental Chemistry, 2010; pp. 37–41.

Ahmed MB, Meki A, AbdRaboh N,Neurotoxic effect of lead on rats: Relationship to Apoptosis2013; 7(2), pp. 192–199.

McClain RM, Becker BA, Teratogenicity, fetal toxicity, and placental transfer of lead nitrate in rats. Toxicol Appl Pharmacol 1975; 31: 72–82.

Azza AA, Hebatalla IA, Asmaa IAD, Protective effect of Zinc against Postnatal Lead-Induced alterations in the Neurobehavioural Development of Normally-Fed and Protein Malnourished Rats. Journal of Neurology and Neuroscience2016; 7(3):117.

Goma UE, Mahrous AA, Changes in Behavior and Learning Ability of Rats Intoxicated with Lead. International Journal of Animal and Veterinary Sciences2013; 7(12). 1096-1102

Basha MR, Murali M, Siddiqi HK, GhosalK, Siddiqi OK, Lashuel HA, Zawia NH, Lead (pb) exposure and its effect on APP proteolysis and A# aggregation. FASEB Journal2005; 19(14), 2083-2084.

Reddy GR, Devi BC, Chetty CS. Developmental lead neurotoxicity: Alterations in brain cholinergic system, NeuroToxicology2007; 28; 402–407.

Bunn TL, Parsons PJ, Kao E,Dietert RR. Exposure to lead during critical windows of embryonic development: differential immunotoxic outcome based on stage of exposure and gender. Toxicol. Sci.2001; 64, 57–66.

Barkur RR, Bairy LK. Evaluation of passive avoidance learning and spatial memory in rats exposed to low levels of lead during specific periods of early brain development. International Journal of Occupational Medicine and Environmental Health2015, 28(3):533 – 544.

Bancroft JD, Gamble M.Theory and Practice of Histological Techniques. 6th Edition, Churchill Livingstone, Elsevier, London. 2008.

Bremner AJ, Spence C. Advances on child development and behavior,’ science direct2017;viewed 15th april 2019 from https://www.sciencedirect.com/topics/.medicine-and-dentistry/palmar-grasp-reflex

Highab SM, Magaji RA, Muhammad, Effect of Lead Poisoning and Antidepressant Drug on the Cerebral Cortex of the Wistar Rats’: Acta Scientific Pharmaceutical Sciences2018; 2(5): 16-21.

Jwad MB,Alwan MJ. Toxicological Effects of Lead Acetate on the Brain of Male Mice’: Proceeding of the Eleventh Veterinary Scientific Conference2012; 1:340-346.

Nuhu S. Effect of Lead on the Histology of Medulla Oblongata, Body Weight and Haematological Profile in Albino Wistar Rats. M.Sc, Bayero University, Kano, Nigeria, 2017.

Anitha KS. Effect of water pollution on LDH Isoenzymes in Fish from Hussain Sagar Lake. Ph.D, Osmania University, Hyderabad, India, 1998.

Jagannath BMT. Effect of Heavy Metals on the Histopathology of Gills and Brain of Fresh Water Fish Catla catla. Bio. Pharm. J., 2013; 6 (1): 99-105.

Luthman J, Oskarsson A, Olson L, Hoffer B. Postnatal lead exposure affects motor skills and exploratory behavior in rats. Environmental Research1992; 58(1-2): 236–252.

Grant LD, Kimmel CA, West GL, Martinez-Vargas CM, Howard JL. Chronic low-level lead toxicity in the rat. Toxicology and Applied Pharmacology1980; 56(1): 42–58.

Dietrich KN, Berger OG, Succop PA, Hammond PB, Bornschein RL. The developmental consequences of low to moderate prenatal and postnatal Pb exposure: intellectual attainment in the Cincinnati Pb Study Cohort following school entry. Neurotoxicol. Teratol 1993; 15: 37–44.

Published

07-10-2021

How to Cite

Nuhu, S. (2021). Prenatal and postnatal effect of lead acetate on the histology of frontal cortex and motor activity in Wistar rats. Innovare Journal of Medical Sciences, 9(6). Retrieved from https://innovareacademics.in/journals/index.php/ijms/article/view/43098

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Original Article(s)